Browsing by Subject "CULTURE"

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  • Reyes-Garcia, Victoria; Garcia-del-Amo, David; Benyei, Petra; Fernandez-Llamazares, Alvaro; Gravani, Konstantina; Junqueira, Andre B.; Labeyrie, Vanesse; Li, Xiaoyue; Matias, Denise M. S.; McAlvay, Alex; Mortyn, Peter Graham; Porcuna-Ferrer, Anna; Schlingmann, Anna; Soleymani-Fard, Ramin (2019)
    Bringing insights from Indigenous and local knowledge into climate change research requires addressing the transferability, integration, and scalability of this knowledge. Using a review of research on place-based observations of climate change impacts, we explore ways to address these challenges. Our search mostly captured scientist-led qualitative research, which - while facilitating place-based knowledge transferability to global research - did not include locally led efforts documenting climate change impacts. We classified and organized qualitative multi-site place-based information into a hierarchical system that fosters dialogue with global research, providing an enriched picture of climate change impacts on local social-ecological systems. A network coordinating the scalability of place-based research on climate change impacts is needed to bring Indigenous and local knowledge into global research and policy agendas.
  • Ryynänen, Toni; Heinonen, Visa (2021)
    Purpose Temporal consumption experiences have been conceptualised as universal, subjective or practice-based experiences. Little research, though, addresses such experiences in conjunction with the repeated and situational consumption events that bring them about. The purpose of this paper is to extend current knowledge by examining how the temporal and situational intertwine during consumption events. For this purpose, the concept of a consumption timecycle based on the research data is constructed. Design/methodology/approach The paper takes a longitudinal and researcher-led approach to study temporal consumption experiences. The data was collected through participant observations, video recordings and personal subjective introspections during three consecutive annual Nordic motorcycle consumer trade shows (2014-2016). The data was analysed using an interpretive approach. Findings The results demonstrate five temporalities that characterise a consumption timecycle as follows: emerging, core, intensifying, fading and idle-time temporalities. The features of these temporal experiences are presented in the conclusions section of the paper. Research limitations/implications Recalled temporal experiences are mediated experiences and they differ from lived experiences. The transferability or generalisability of the results might be limited, as the case is situated in the Nordic context. Originality/value The paper presents the novel concept of a consumption timecycle that extends current debates about consumer time. The consumption timecycle is contrasted with established temporal concepts in consumer and marketing research.
  • Barmaki, Samineh; Jokinen, Ville; Obermaier, Daniela; Blokhina, Daria; Korhonen, Matti; Ras, Robin H. A.; Vuola, Jyrki; Franssila, Sami; Kankuri, Esko (2018)
    Physiological oxygen levels within the tissue microenvironment are usually lower than 14%, in stem cell niches these levels can be as low as 0-1%. In cell cultures, such low oxygen levels are usually mimicked by altering the global culture environment either by O-2 removal (vacuum or oxygen absorption) or by N-2 supplementation for O-2 replacement. To generate a targeted cellular hypoxic microenvironment under ambient atmospheric conditions, we characterised the ability of the dissolved oxygen-depleting sodium sulfite to generate an in-liquid oxygen sink. We utilised a microfluidic design to place the cultured cells in the vertical oxygen gradient and to physically separate the cells from the liquid. We demonstrate generation of a chemical in-liquid oxygen sink that modifies the surrounding O-2 concentrations. O-2 level control in the sink-generated hypoxia gradient is achievable by varying the thickness of the polydimethylsiloxane membrane. We show that intracellular hypoxia and hypoxia response element-dependent signalling is instigated in cells exposed to the microfluidic in-liquid O-2 sink-generated hypoxia gradient. Moreover, we show that microfluidic flow controls site-specific microenvironmental kinetics of the chemical O-2 sink reaction, which enables generation of intermittent hypoxia/re-oxygenation cycles. The microfluidic O-2 sink chip targets hypoxia to the cell culture microenvironment exposed to the microfluidic channel architecture solely by depleting O-2 while other sites in the same culture well remain unaffected. Thus, responses of both hypoxic and bystander cells can be characterised. Moreover, control of microfluidic flow enables generation of intermittent hypoxia or hypoxia/re-oxygenation cycles. (C) 2018 Published by Elsevier Ltd on behalf of Acta Materialia Inc.
  • Narva, Elisa; Stubb, Aki; Guzman, Camilo; Blomqvist, Matias; Balboa, Diego; Lerche, Martina; Saari, Markku; Otonkoski, Timo; Ivaska, Johanna (2017)
    Cell-type-specific functions and identity are tightly regulated by interactions between the cell cytoskeleton and the extracellular matrix (ECM). Human pluripotent stem cells (hPSCs) have ultimate differentiation capacity and exceptionally low-strength ECM contact, yet the organization and function of adhesion sites and associated actin cytoskeleton remain poorly defined. We imaged hPSCs at the cell-ECM interface with total internal reflection fluorescence microscopy and discovered that adhesions at the colony edge were exceptionally large and connected by thick ventral stress fibers. The actin fence encircling the colony was found to exert extensive Rho-ROCK-myosin-dependent mechanical stress to enforce colony morphology, compaction, and pluripotency and to define mitotic spindle orientation. Remarkably, differentiation altered adhesion organization and signaling characterized by a switch from ventral to dorsal stress fibers, reduced mechanical stress, and increased integrin activity and cell-ECM adhesion strength. Thus, pluripotency appears to be linked to unique colony organization and adhesion structure.
  • Kouhia, Anna (2012)
    The meaning and value of crafts as bearers of the cultural heritage are widely acknowledged, and deserved attention has also been paid to the pedagogical applications of craft in practice. Still, research relating to subjectively interpreted meanings of crafts has remained rather scarce. The main interest of the study is to examine subjectively construed meanings of craft associated both to the self-reflective act of making and to the tangible craft object, and categorize the discovered meanings into representative categories. In presenting a multi-perspectival framework for the categories that portray the common kinds of meanings that crafts have, a substance that surpasses cultural boundaries is also captured due to the multicultural research setting. The primary empirical data of the study are composed of interviews of six women from different cultural backgrounds; the supporting data feature a participant observation of a multicultural craft activity group and photographic notes. The wide range of different meanings captured from the data is, during the conducted qualitative content analysis, critically classified into representational categories that typify the personally experienced meanings. The analysis revealed eight interrelated meaning categories regarding the meanings of craft that go beyond cultural boundaries: Meanings proceed from the subjective process of interpreting craft in a socially constructed, personalized setting, but still the meanings given to craft have been given similar characterizations in the several cultural contexts examined. This study adds to an understanding of how personalized meanings contribute to building intercultural reciprocity.
  • Kögler, Martin; Paul, Andrea; Anane, Emmanuel; Birkholz, Mario; Bunker, Alex; Viitala, Tapani; Maiwald, Michael; Junne, Stefan; Neubauer, Peter (2018)
    The application of Raman spectroscopy as a monitoring technique for bioprocesses is severely limited by a large background signal originating from fluorescing compounds in the culture media. Here, we compare time-gated Raman (TG-Raman)-, continuous wave NIR-process Raman (NIR-Raman), and continuous wave micro-Raman (micro-Raman) approaches in combination with surface enhanced Raman spectroscopy (SERS) for their potential to overcome this limit. For that purpose, we monitored metabolite concentrations of Escherichia coli bioreactor cultivations in cell-free supernatant samples. We investigated concentration transients of glucose, acetate, AMP, and cAMP at alternating substrate availability, from deficiency to excess. Raman and SERS signals were compared to off-line metabolite analysis of carbohydrates, carboxylic acids, and nucleotides. Results demonstrate that SERS, in almost all cases, led to a higher number of identifiable signals and better resolved spectra. Spectra derived from the TG-Raman were comparable to those of micro-Raman resulting in well-discernable Raman peaks, which allowed for the identification of a higher number of compounds. In contrast, NIR-Raman provided a superior performance for the quantitative evaluation of analytes, both with and without SERS nanoparticles when using multivariate data analysis. (c) 2018 American Institute of Chemical Engineers
  • Auvinen, Vili-Veli; Merivaara, Arto; Kiiskinen, Jasmi; Paukkonen, Heli; Laurén, Patrick; Hakkarainen, Tiina; Koivuniemi, Raili; Sarkanen, Riina; Ylikomi, Timo; Laaksonen, Timo; Yliperttula, Marjo (2019)
    The aim of this study was to evaluate the effects of two nanofibrillated cellulose (NFC) hydrogels on two human derivatives during freeze-drying. Native NFC hydrogel is a suitable platform to culture 3D cell spheroids and a hydrogel processed further, called anionic NFC (ANFC) hydrogel, is an excellent platform for controlled release of proteins. Moreover, it has been shown to be compatible with freeze-drying when correct lyoprotectants are implemented. Freeze-drying is a method, where substance is first frozen, and then vacuum dried trough sublimation of water in order to achieve dry matter without the loss of the original three-dimensional structures. The first chosen human derivative was adipose tissue extract (ATE) which is a cell-free growth factor-rich preparation capable of promoting growth of regenerative cells. The release of growth factors from the freeze-dried mixture of ATE and ANFC was compared to that of non-freeze-dried control mixtures. The release profiles remained at the same level after freeze-drying. The second derivative was hepatocellular carcinoma (HepG2) cell spheroids which were evaluated before and after freeze-drying. The 3D structure of the HepG2 cell spheroids was preserved and the spheroids retained 18% of their metabolic activity after rehydration. However, the freeze-dried and rehydrated HepG2 cell spheroids did not proliferate and the cell membrane was damaged by fusion and formation of crystals.
  • Kornilov, Roman; Puhka, Maija; Mannerström, Bettina; Hiidenmaa, Hanna; Peltoniemi, Hilkka; Siljander, Pia Riitta-Maria; Seppänen-Kaijansinkko, Riitta; Kaur, Sippy (2018)
    Fetal bovine serum (FBS) is the most commonly used supplement in studies involving cell-culture experiments. However, FBS contains large numbers of bovine extracellular vesicles (EVs), which hamper the analyses of secreted EVs from the cell type of preference and, thus, also the downstream analyses. Therefore, a prior elimination of EVs from FBS is crucial. However, the current methods of EV depletion by ultracentrifugation are cumbersome and the commercial alternatives expensive. In this study, our aim was to develop a protocol to completely deplete EVs from FBS, which may have wide applicability in cell-culture applications. We investigated different EVdepleted FBS prepared by our novel ultrafiltration-based protocol, by conventionally used overnight ultracentrifugation, or commercially available depleted FBS, and compared them with regular FBS. All sera were characterized by nanoparticle tracking analysis, electron microscopy, Western blotting and RNA quantification. Next, adipose-tissue mesenchymal stem cells (AT-MSCs) and cancer cells were grown in the media supplemented with the three different EV-depleted FBS and compared with cells grown in regular FBS media to assess the effects on cell proliferation, stress, differentiation and EV production. The novel ultrafiltration-based protocol depleted EVs from FBS clearly more efficiently than ultracentrifugation and commercial methods. Cell proliferation, stress, differentiation and EV production of AT-MSCs and cancer cell lines were similarly maintained in all three EV-depleted FBS media up to 96 h. In summary, our ultrafiltration protocol efficiently depletes EVs, is easy to use and maintains cell growth and metabolism. Since the method is also cost-effective and easy to standardize, it could be used in a wide range of cell-culture applications helping to increase comparability of EV research results between laboratories.
  • Bjorninen, Miina; Gilmore, Kerry; Pelto, Jani; Seppänen-Kaijansinkko, Riitta; Kellomaki, Minna; Miettinen, Susanna; Wallace, Gordon; Grijpma, Dirk; Haimi, Suvi (2017)
    We investigated the use of polypyrrole (PPy)-coated polymer scaffolds and electrical stimulation (ES) to differentiate adipose stem cells (ASCs) towards smooth muscle cells (SMCs). Since tissue engineering lacks robust and reusable 3D ES devices we developed a device that can deliver ES in a reliable, repeatable, and cost-efficient way in a 3D environment. Long pulse (1 ms) or short pulse (0.25 ms) biphasic electric current at a frequency of 10 Hz was applied to ASCs to study the effects of ES on ASC viability and differentiation towards SMCs on the PPy-coated scaffolds. PPy-coated scaffolds promoted proliferation and induced stronger calponin, myosin heavy chain (MHC) and smooth muscle actin (SMA) expression in ASCs compared to uncoated scaffolds. ES with 1 ms pulse width increased the number of viable cells by day 7 compared to controls and remained at similar levels to controls by day 14, whereas shorter pulses significantly decreased viability compared to the other groups. Both ES protocols supported smooth muscle expression markers. Our results indicate that electrical stimulation on PPy-coated scaffolds applied through the novel 3D ES device is a valid approach for vascular smooth muscle tissue engineering.
  • Ghahramani, Abolfazl (2016)
    Many organizations are adapting to the requirements of occupational health and safety management systems worldwide. Despite the considerable acceptance of the systems for managing occupational health and safety in organizations, there is still no clear consensus on their effectiveness. The present study aimed to identify potential areas for improvements based on the experience and perception of the managers who worked in companies that are adopting the occupational health and safety assessment series 18001 standard in Iran. Sixteen semi-structured interviews were conducted with the managers. A qualitative study design using a grounded theory approach was used to analyze the gathered data. Eleven categories emerged to explain the influencing factors that hinder or facilitate the effectiveness of the standard in the companies: (1) management commitment; (2) safety communication; (3) employee involvement; (4) integration; (5) training; (6) safety culture; (7) internal incentives; (8) enforcement; (9) occupational health and safety authority' support, (10) auditing, and (11) external incentives. Moreover, a conceptual model was developed based on the categories. The commitment of the senior managers to safety and their support of the system in practice can facilitate the improvement of the adaptation to the standard in the companies. Conducting efforts to train employees about occupational health and safety and to involve them in the practices required by the standard can help the companies in creating a positive safety culture. The development of a new inspection program with more enforcement on occupational health and safety legislation by the authorities would help the companies to adopt the requirements of the legislation. The application of an enforced policy by accreditation bodies to check the third-party auditing process could also increase the quality of auditing and help to improve the effectiveness of their systems to achieve a better occupational health and safety performance. (C) 2016 Elsevier Ltd. All rights reserved.
  • Franks, Victoria R.; Ewen, John G.; McCready, Mhairi; Thorogood, Rose (2020)
    Early independence from parents is a critical period where social information acquired vertically may become outdated, or conflict with new information. However, across natural populations, it is unclear if newly independent young persist in using information from parents, or if group-level effects of conformity override previous behaviours. Here, we test if wild juvenile hihi (Notiomystis cincta, a New Zealand passerine) retain a foraging behaviour from parents, or if they change in response to the behaviour of peers. We provided feeding stations to parents during chick-rearing to seed alternative access routes, and then tracked their offspring's behaviour. Once independent, juveniles formed mixed-treatment social groups, where they did not retain preferences from their time with parents. Instead, juvenile groups converged over time to use one access route­ per group, and juveniles that moved between groups switched to copy the locally favoured option. Juvenile hihi did not copy specific individuals, even if they were more familiar with the preceding bird. Our study shows that early social experiences with parents affect initial foraging decisions, but social environments encountered later on can update transmission of arbitrary behaviours. This suggests that conformity may be widespread in animal groups, with potential cultural, ecological and evolutionary consequences.
  • Sandström, Niclas; Nevgi, Anne (2019)
    This paper took a pedagogical campus developer’s look into a campus retrofitting process. The paper presents a case study of a major Finnish research-intensive university. The data consist of semi-structured interviews of information-rich key stakeholders identified using snowball sampling method. The findings suggest that co-design should be followed through the whole retrofitting process with sufficient communications between stakeholders. The study introduces the concept of learning landscape reliability, putting digital age basic needs in the centre of learning landscape usability.
  • Jallinoja, Piia Tuuli; Niva, Mari Helena; Latvala, Terhi Tuulikki (2016)
    A transition towards more sustainable food consumption requires changes in everyday eating patterns, particularly a substitution of animal protein with plant-based protein sources. However, in many European countries plant protein consumption is low compared to meat consumption. The article explores plant protein consumption frequencies, future intentions to increase bean consumption, and the associations of frequent bean eating with socioeconomic factors and beanrelated meanings, material issues and competence. A population web-based survey was conducted in 2013 among 15-64-year-old Finns (n=1048). The results showed that beans and soy-based plant proteins were infrequently consumed. A fifth of the respondents intended to increase their bean consumption in the future, intention being the greatest among those who already included beans in their diets. Frequent bean consumption was most likely among persons aged 25-34, living around the capital district, with education higher than comprehensive or vocational school, and who were vegetarian. Perceiving beans as culturally acceptable and good-tasting, and having competence in preparing bean meals were positively associated with the frequent eating of beans. The results suggest that for plant proteins to replace meat, new meanings and competences related to preparing and eating pulse-based dishes are needed. Based on our results, we build alternative future scenarios for plant protein consumption and the related requirements for changes. Several actor groups, such as NGOs, politicians, celebrity chefs and teachers of home economics have a central role in the developments.
  • Özdemir, Meltem; Caglayan, Feriha; Bigger, Floris; Pussinen, Pirkko; Könönen, Eija; Yamalik, Nermin; Gursoy, Mervi; Fteita, Dareen; Nazmi, Kamran; Güncü, Güliz N.; Pietiäinen, Milla; Tolvanen, Mimmi; Gürsoy, Ulvi Kahraman (2020)
    Aim To profile gingival tissue levels of human beta-defensin (hBD)-2 and hBD-3 in relation to gingival inflammation, Th17-related cytokine concentrations, Porphyromonas gingivalis counts, and gingipain and total protease activities. Materials and Methods Gingival tissue and subgingival plaque samples were collected from 21 periodontitis patients including 48 periodontal pocket sites with marginal, mild, or moderate to severe inflammation. hBD levels were determined by immunodetection, P. gingivalis counts with real-time polymerase chain reaction, protease activities with fluorogenic substrates, and cytokine concentrations with Luminex technique. Data were statistically analysed using Kruskal-Wallis and Mann-Whitney U tests and Spearman correlation coefficients. Results Subgingival plaque counts of P. gingivalis (p = .001) and gingipain activity (p <.001), as well as interleukin (IL)-1 beta (p = .012), IL-10 (p = .024), IL-17A (p = .002), IL-17F (p = .006), and IL-23 (p = .036) concentrations were elevated in severely inflamed sites, whereas no change was observed in hBD-2 and hBD-3 levels. Negative correlations were found between protease activity and hBD-2 (p = .033) and hBD-3(p = .003) levels. Conclusions Shift in gingival inflammation from marginal to mild stage is related to elevations in subgingival plaque P. gingivalis counts and gingipain activity, but not to tissue hBD levels. Negative correlations between hBDs and total protease activity suggest the degradation of these antimicrobial peptides in progressed inflammation.
  • Kangas, Emilia; Lämsä, Anna-Maija; Jyrkinen, Marjut (2019)
    It has been claimed that in the context of organizations and management, fathers are invisible. One source of tension for fathers who work and who want to participate in family life is that even though involved fatherhood is emerging in many western societies, a family-oriented male identity is likely to be problematic for men in organizations. This article aims to contribute to a better understanding of a professional and managerial men's work-family relationship using discourse analysis on data from three different media sources in Finland, published during 1990-2015. We identified two competing discourses: one of stasis, the other of change. The stasis discourse is constructed around traditionally masculine management and fatherhood roles, while the changing discourse embodies more diverse masculinities and fatherhood. We conclude that although the discourse on fatherhood in the organizational context is moving towards gender equality, at the same time a strong discourse is putting a brake on such development, especially regarding management.
  • Mogollon, Isabel; Ahtiainen, Laura (2020)
    Embryonic development of ectodermal organs involves a very dynamic range of cellular events and, therefore, requires advanced techniques to visualize them. Ectodermal organogenesis proceeds in well-defined sequential stages mediated by tissue interactions. Different ectodermal organs feature shared morphological characteristics, which are regulated by conserved and reiterative signaling pathways. A wealth of genetic information on the expression patterns and interactions of specific signaling pathways has accumulated over the years. However, the conventional developmental biology methods have mainly relied on two-dimensional tissue histological analyses at fixed time points limiting the possibilities to follow the processes in real time on a single cell resolution. This has complicated the interpretation of cause and effect relationships and mechanisms of the successive events. Whole-mount tissue live imaging approaches are now revealing how reshaping of the epithelial sheet for the initial placodal thickening, budding morphogenesis and beyond, involve coordinated four dimensional changes in cell shapes, well-orchestrated cell movements and specific cell proliferation and apoptosis patterns. It is becoming evident that the interpretation of the reiterative morphogenic signals takes place dynamically at the cellular level. Depending on the context, location, and timing they drive different cell fate choices and cellular interactions regulating a pattern of behaviors that ultimately defines organ shapes and sizes. Here we review how new tissue models, advances in 3D and live tissue imaging techniques have brought new understanding on the cell level behaviors that contribute to the highly dynamic stages of morphogenesis in teeth, hair and related ectodermal organs during development, and in dysplasia contexts.
  • Mohlin, Sofie; Kunttas, Ezgi; Persson, Camilla U.; Abdel-Haq, Reem; Castillo, Aldo; Murko, Christina; Bronner, Marianne E.; Kerosuo, Laura (2019)
    Neural crest cells have broad migratory and differentiative ability that differs according to their axial level of origin. However, their transient nature has limited understanding of their stem cell and self-renewal properties. While an in vitro culture method has made it possible to maintain cranial neural crest cells as self-renewing multipotent crestospheres (Kerosuo et al., 2015), these same conditions failed to preserve trunk neural crest in a stem-like state. Here we optimize culture conditions for maintenance of avian trunk crestospheres, comprised of both neural crest stem and progenitor cells. Our trunk-derived crestospheres are multipotent and display self-renewal capacity over several weeks. Trunk crestospheres display elevated expression of neural crest cell markers as compared to those characteristic of ventrolateral neural tube or mesodermal fates. Moreover, trunk crestospheres express increased levels of trunk neural crest-enriched markers as compared to cranial crestospheres. Finally, we use lentiviral transduction as a tool to manipulate gene expression in trunk crestospheres. Taken together, this method enables long-term in vitro maintenance and manipulation of multipotent trunk neural crest cells in a premigratory stem or early progenitor state. Trunk crestospheres are a valuable resource for probing mechanisms underlying neural crest sternness and lineage decisions as well as accompanying diseases.
  • Sarkia, Matti; Kaidesoja, Tuukka; Hyyryläinen, Mikko (2020)
    Discussions of the relations between the social sciences and the cognitive sciences have proliferated in recent years. Our article contributes to the philosophical and methodological foundations of the cognitive social sciences by proposing a framework based on contemporary mechanistic approaches to the philosophy of science to analyze the epistemological, ontological and methodological aspects of research programs at the intersection of the social sciences and the cognitive sciences. We apply this framework to three case studies which address the phenomena of social coordination, transactive memory, and ethnicity. We also assess how successful these research programs have been in providing mechanistic explanations for these phenomena, and where more work remains to be done.
  • Rommel, Carl Anders Truls (2018)
    This article explores men at a state-owned youth center in Cairo, struggling to cope with uncertainties and change in the aftermath of Egypt's January 2011 Revolution. Conceptually, the article critically engages anthropologist Laura Bear's suggestion that an ethics of productivity saturate neoliberal masculinity. As my ethnographic stories about football coaches and state bureaucrats illustrate, being a good man recurrently surfaced as a problem of how to work productively in and on time: as ambiguities between discordant futures that left material needs, familiar care, and development of football talents difficult to reconcile. Often, my interlocutors linked this conundrum to a wide-ranging opacity, conjured as corruption (fisad). My analysis of this male predicament allows me to spotlight one of the Egyptian revolution's most luring promises: a transparent and meritocratic system, where a man's work would finally be allowed to work on all futures deemed morally and materially significant.
  • Härmä, Ville; Haavikko, Raisa; Virtanen, Johannes; Ahonen, Ilmari; Schukov, Hannu-Pekka; Alakurtti, Sami; Purev, Enkhee; Rischer, Heiko; Yli-Kauhaluoma, Jari; Moreira, Vânia M.; Nees, Matthias; Oksman-Caldentey, Kirsi-Marja (2015)
    The anti-invasive and anti-proliferative effects of betulins and abietane derivatives was systematically tested using an organotypic model system of advanced, castration-resistant prostate cancers. A preliminary screen of the initial set of 93 compounds was performed in two-dimensional (2D) growth conditions using non-transformed prostate epithelial cells (EP156T), an androgen-sensitive prostate cancer cell line (LNCaP), and the castration-resistant, highly invasive cell line PC-3. The 25 most promising compounds were all betulin derivatives. These were selected for a focused secondary screen in three-dimensional (3D) growth conditions, with the goal to identify the most effective and specific anti-invasive compounds. Additional sensitivity and cytotoxicity tests were then performed using an extended cell line panel. The effects of these compounds on cell cycle progression, mitosis, proliferation and unspecific cytotoxicity, versus their ability to specifically interfere with cell motility and tumor cell invasion was addressed. To identify potential mechanisms of action and likely compound targets, multiplex profiling of compound effects on a panel of 43 human protein kinases was performed. These target de-convolution studies, combined with the phenotypic analyses of multicellular organoids in 3D models, revealed specific inhibition of AKT signaling linked to effects on the organization of the actin cytoskeleton as the most likely driver of altered cell morphology and motility.