Browsing by Subject "Husdjursvetenskap"

Sort by: Order: Results:

Now showing items 1-20 of 166
  • Lahtinen, Sonja (Helsingin yliopisto, 2020)
    The purpose of the study was to determine the possibilities of utilizing the by-product streams of fish processing industry in the production of fish oil and silage. In this thesis the effect of different antioxidants on the preservation of salmon scraps was studied and the utilisation of the dregs from which the oil was extracted as feed was considered. The scarps preserved in this study were from farmed salmon and consisted of internal organs and roe. The study had two experiments. The first one lasted for 65 days and in it formic acid was added to the scraps for a final acid content of 3 %. The scraps with only the formic acid acted as the control and the four different antioxidants studied were butylated hydroxyanisole (BHA), tert-butylhydroquinone (TBHQ), ethoxyquin mixture (Helmlox) and butylated hydroxytoluene (Ionol). The amount of Ionol was 100 ppm and for BHA, TBHQ and Helmlox there were two different amounts, 50 and 100 ppm. The second experiment lasted for 29 days and the formic acid content was 1 %. BHA and Helmlox antioxidants were used with an amount of 10 ppm. The oil and sediment parts from the experiments were analyzed separately. Based on the analyses the antioxidants or their amounts did not have an effect on the values extracted from the sediment. In the first experiment the analyses from the oil gave mostly highly significant (p<0,001) differences between the control and the antioxidants. There were also clear differences between the antioxidants but the different amounts did not have an effect on the AV-, PV- and TOTOX-values. In the second experiment the analyses from the oil gave only minor differences between the control and the antioxidants, the differences between different antioxidants being larger. The peroxide value of BHA was clearly larger than that of the control and Helmlox and therefore also the TOTOX-value was larger, which contradicts the results of the first experiment. In the second experiment also the measured amino acid contents diminished when the antioxidants were added which contradicts previous research. TBHQ worked best as an antioxidant, then BHA, Ionol and Helmlox in order. The usage of the dregs from the scraps as feed for pigs and chicken is limited by its high fish oil content. However for fur animals salmon scraps are suitable as feed products if the amino acid deficiencies are compensated with other feed products.
  • Siipola, Anne (Helsingin yliopisto, 2019)
    Conservation of the genetic diversity enables population adaption to climate and production system changes and prevents harmful consequences of inbreeding. The aim of this study was to investigate the state of genetic diversity in Finnish Ayrshire and Finncattle breeds (Western, Eastern and Northern Finncattle) based on pedigree information. The data were received from FABA co-op. Average inbreeding coefficient and relationships were estimated for animals born between 1970 and 2017. Effective population sizes were estimated separately for each birth-year group. In addition, genetic contribution of the most important ancestors of the population were investigated. The Software packages RelaX2 and R-program were used to estimate the population parameters. Average inbreeding coefficient had either stayed at the same level or decreased for all breeds except Western Finncattle. Average inbreeding coefficient of Western Finncattle has increased 2.2 %-units during the last 20 years. In addition, average relationships between breeding animals has remained stable in other breeds except Western Finncattle. Estimated effective population sizes were 163 (AY), 78 (PSK) 74 (LSK) and 57 (ISK) for animals born between 2015 and 2017. The most important ancestors to animals born between 2015 and 2017 of each breed were A Lier (AY), Opari (LSK), Asa (ISK) and Fager (PSK). Effective population sizes of different Finncattle breeds were larger than the minimum recommendation of 50 and average relationship coefficients between breeding animals have stabilized during the past few years. Despite this, the diversity of all Finncattle breeds needs to be strictly monitored. The genetic diversity of Finnish Ayrshire is at the sustainable level that is partly due to cooperation of the Nordic breeding organizations. In the future, genomic information will hopefully provide more effective tools to estimate and manage population genetic diversity.
  • Sampolahti, Sani (Helsingfors universitet, 2014)
    In this study the economic values for the breeding goal traits for Finnish Ayrshire cattle were determined by the bioeconomical model. Additionally, the economic value was determined for a new trait, feed efficiency, which was modelled as residual feed intake (RFI). Feed efficiency was added to breeding goals because of intrest in reduction of harmful environmental effects of dairy cattle production. The effect of increasing feed costs on the economic weights was also studied. Economic values were determined by the program ECOWEIGHT. The bioeconomical model included 21 traits, which can be divided a few categories: milk production traits, growth and carcass traits and functional traits (calving difficulty, stillbirth, calf mortality in the rearing period, fertility traits, productive lifetime of cows, incidence of clinical mastitis, somatic cell score (SCS) and residual feed intake (RFI)). Economic values and standardized economic weights were defined for the traits. Profitability of the milk production was negative (?13,3%), when the subsidies were not included in the calculations. Adding RFI didn’t have any effect on the economic values of the other traits and it didn’t change the order of the traits in standardized economical weight. The marginal economic values for RFI of cows and heifers were ?55,8 and ?24,9 €/kg/d, respectively. The highest relative economic weights was found for 305-d milk (36%), protein (14%), fat (9%), mature weight (7%) and calving interval (5%). Relative economic weights for RFI of cows and heifers were 4,6% and 1,5%, respectively. An increase in the feeding costs (10, 30 and 50%) changed the order of the traits in standardized economical weights. The weights of milk production traits were decreased and the weights of growth traits, RFI and calving interval were increased. RFI of cows was the fifth important trait when feeding costs increased 30%. According to the results of this study would be beneficial, especially if the relative im-portance of feed efficiency will increase in the future due to increasing feed costs or in-creasing requirements to mitigate the environmental impact of milk production. However, more research will be needed before adding RFI to the breeding goals.
  • Nyqvist, Krista (Helsingin yliopisto, 2018)
    Tiivistelmä/Referat – Abstract The long period of indoor feeding in Finland emphasizes the importance of good quality silage in dairy cow nutrition. Silage additives are used to ensure successful ensiling. Silage inoculants enhance silage lactic acid fermentation when lactic acid bacteria and sometimes enzymes and other fermentative bacteria are added to silage. Lactic acid content has been higher and pH lower in silages treated with inoculant than in silages without any additive. Aerobic stability of silage and total mixed ration (TMR) has been better with silage treated using inoculant compared to silage without additives. In some studies, the digestibility of dry matter and organic matter has been better with inoculant-treated silage. Silage dry matter intake has been improved with silage treated with inoculants in studies performed with dairy cows, growing beef cattle and sheep. Milk yield has increased in some studies with biological additive when compared to silage without additives. The aim of this study was to examine whether ensiling grass silage with biological additive influences feed intake or milk production of dairy cows in comparison to silage without inoculant. The study was conducted at the research farm of University of Helsinki. First cut timothy – meadow fescue sward was cut and wilted for 46 hours. The grass was harvested in round bales wrapped with 8 film layers either using silage inoculant (treatment B, with lactic acid bacteria and enzymes) or without additive (treatment A). A series of five bales of each silage were prepared one after another. The grass had dry matter of 183 g/kg before wilting and 328 g/kg after wilting. Water soluble carbohydrate concentration was 121 g/kg dry matter (DM). The silages were stored for 174 days before the beginning of the feeding trial. There were no differences in silage fermentation quality. Both silages had high concentration of lactic acid (100 g/kg DM). The dry matter contents were 296 and 294 g/kg and pH 4.25 and 4.31 for the inoculated silage and the silage without additive, respectively. Eight Finnish Ayrshire cows (95 days in milk, standard deviation 25.9) of second or later parity were used. The cows were kept in tie stalls during the trial. A double-reversal design was used with two treatments sequences (ABBA or BAAB) of 21 days each. The cows were fed with total mixed ration consisting of 65% (dry matter basis) of silage (inoculated or without additive), 20% of concentrate mixture, 13% rapeseed meal and 1.5% mineral supplement. Dry matter intake was 1,1 kg/d higher with the inoculated silage (p=0.01) than with untreated silage. Digestibility of dietary dry matter (p=0.02) and organic matter (p=0.02) was lower with inoculated than untreated silage. There was no difference in milk production between treatments (p=0.65). This study indicates that good fermentation quality of wilted (DM 300 g kg) grass silage can be achieved without additives. The increased dry matter intake or the lower digestibility of the diet containing inoculated silage cannot be explained by the silage composition or fermentation profile.
  • Ivanov, Dea (Helsingfors universitet, 2017)
    Tämän maisterintutkielman tavoitteena oli selvittää eroja viiden kaupallisen broilerihybridin kävelykyvyssä ja aktiivisuudessa. Hypoteesina oli, että broilerihybridien väliset erot näkyvät kävelytestin ja muunnellun latency to lie- eli LTL-testin tuloksissa, videomateriaalissa ja tuotantotuloksissa. Kukkoja oli viittä kaupallista, yleisesti käytettyä broilerihybridiä, joista yksi oli hitaampikasvuinen, luomu- ja free range -tuotantoon käytetty hybridi. Sukupuolilajitellut 350 kukkoa (65-75 lintua/hybridi) jaettiin satunnaisesti 25 numeroituun karsinaan hybrideittäin (5 karsinaa/hybridi). Lintujen rehunkulutusta ja kasvua seurattiin koko kokeen ajan viikottain. Lintujen kävelykyky arvioitiin teuraspäivinä kävelytestillä ja muunnellulla LTL-testillä (koepäivät 15, 19, 22, 32, 36, 43 ja 49). Kaikille linnuille tehtiin ylimääräinen kävelytesti myös päivinä 27 ja 40. Lintujen käyttäytymistä videoitiin koepäivinä 12, 17, 25, 34 ja 41. Tarkkailtaviksi valittiin satunnaisesti kuusi värjättyä lintua per hybridi (yhteensä 30 lintua). Videoista analysoitiin jokaiselta päivältä kaksi 10 minuutin pituista jaksoa, joista kirjattiin ylös muutokset lintujen aktiivisuudessa (lintujen ryömintä- ja kävelysummat, seisoma- ja makuufrekvenssit ja kokonaismakuu- ja seisoma-aika). Lintujen tuotantotulosten ja kävelykyvyn väliset yhteydet testattiin Kendallin korrelaatiolla. Hybridillä oli erittäin merkitsevä vaikutus kävelykykyyn mitattuna kävelytestillä (p= 0,001). Hybridillä ei ollut merkitsevää vaikutusta lintujen muunnellun LTL-testin tuloksiin (p=0,394). Erittäin merkitsevä negatiivinen korrelaatio LTL-testitulokseen oli elopainolla (p=0,000) ja kävelytestin arvosanalla (p=0,000). Hybridillä oli erittäin merkitsevä vaikutus lintujen elopainoon (p=0,000). Hybridillä ei ollut merkitsevää vaikutusta lintujen videoista mitattuun aktiivisuuteen. Lintujen aktiivisuus korreloi negatiivisesti tarkkailupäivän ja kävelytestin arvosanan kanssa. Lintujen suurempi rintalihasten paino (p=0,001), elopaino (p=0,008) ja rintalihassaanto (p=0,008) huononsivat lintujen kävelytestin arvosanaa.
  • Varis, Maiju (Helsingin yliopisto, 2022)
    Suomessa on tarvetta uusille kotimaisille valkuaislähteille. Öljyntuotannosta ylijäävä camelinapuriste vastaa aminohappokoostumukseltaan melko hyvin lypsykarjan ruokinnassa yleisesti käytettyä ryp-siä, joten se voisi sopia hyvin valkuaisrehuksi. Camelina ei tarvitse paljon ravinteita ja sitä pystytään kasvattamaan monenlaisissa ilmasto-oloissa, koska se kestää hyvin vaikeita ympäristöoloja, kuten kuivuutta, sekä tauteja ja tuholaisia. Tutkimuksen tavoitteena oli tutkia camelinan soveltuvuutta valkuaisrehuksi lypsylehmillä. Tutkimus koostui kahdesta osakokeesta, joissa mitattiin ohutsuoleen virtaavan käyttökelpoisen valkuaisen määrää in vitro. Osakokeessa 1 verrattiin kahta eri camelinalajiketta rypsiin ja osakokeen 2 koe-dieetti sisälsi camelinaa nousevina tasoina siten, että camelinavalkuainen korvasi rypsivalkuaista 0, 1/3, 2/3 ja 3/3. Hypoteesi oli, että camelina voi korvata rypsin täysin ilman ohutsuoleen virtaavan käyttökelpoisen valkuaisen määrän vähenemistä. Tutkimuksessa koerehuista analysoitiin perusanalyysien lisäksi aminohappokoostumus ja Cornellin typpifraktiot. Typpifraktioiden perusteella camelinalla ja rypsillä on samankaltainen hajoavuuspoten-tiaali pötsissä. Ohutsuoleen virtaavan käyttökelpoisen valkuaisen määrää mitattiin laboratoriossa Bioprocess control’in Gas Endeavor in vitro –laitteella inkuboimalla rehuja naudan pötsinesteessä. Osakokeiden tulokset olivat keskenään ristiriitaisia. Osakoe 1:n mukaan ohutsuoleen virtaavan käyt-tökelpoisen valkuaisen määrässä ei ollut merkittäviä eroja valkuaisrehujen välillä. Osakoe 2:ssa ohutsuoleen virtaavan käyttökelpoisen valkuaisen määrä yllättäen väheni camelinan annostasoa nostettaessa. Tässä tutkimuksessa hypoteesi ei toteutunut kaikkien tulosten osalta, mutta sekä osakoe 1:n että typpifraktioiden mukaan camelinan pitäisi soveltua rypsin korvaajaksi valkuaisrehu-na. On epäselvää, miksi osakoe 2:ssa tämä ei toteutunut. Tulevaisuudessa tarvitaan lisää tutkimuk-sia camelinan soveltuvuudesta valkuaisrehuksi, ja in vitro -menetelmä on hyvä tutkimuskeino ennen in vivo -kokeita.
  • Salomaa, Sini (Helsingin yliopisto, 2014)
    The aim of this study was to investigate the effects of graded amounts of camelina oil on milk fatty acid composition in lactating cows fed diets based on a mixture of grass and red clover silages. The experiment was carried out at the University of Helsinki research farm in Viikki 30th January 2009 – 23th April 2009. Eight multiparous Finnish Ayrshire cows participated in this experiment and four of them were rumen fistulated. Experimental design used was 4 x 4 Latin square. Treatments consisted of concentrate supplements containing various levels of camelina oil (0%, 2%, 4% and 6% on air-dry basis). All concentrates contained camelina expeller (20% on air-dry basis). The cows were offered daily 12 kg of experimental concentrate and silage ad libitum. The experimental periods lasted for 21 days. The first 14 days were adaptation period and the last 7 days formed sampling period. Increase of camelina oil level in the diet linearly decreased forage and whole diet dry matter intake (P?0,002). Camelina oil level did not affect organic matter, NDF and nitrogen whole-tract digestibility (P>0,10). Milk yield and milk protein- and lactose content linearly decreased when camelina oil level increased (P<0,001). Camelina oil level did not affect milk fat yield (P>0,100). Milk fat content (P=0,014) linearly increased and protein content (P=0,032) and urea content (P<0,001) linearly decreased when camelina oil level increased. Camelina oil level did not affect milk lactose content (P>0,100). Increase of camelina oil level linearly worsened milk taste panel scores (P=0,018). Camelina oil level did not affect plasma metabolite concentrations except that of total free fatty acids that linearly increased with camelina oil supplementation (P<0,001). Effects of camelina oil supplementation on rumen pH and rumen fermentation pattern were numerically negligible. Increase of camelina oil level linearly decreased the concentration of saturated fatty acids in milk fat (P<0,001) and linearly increased those of monounsaturated (P<0,001) and polyunsaturated (P<0,002) fatty acids. Increase of camelina oil level linearly decreased the content of mammary de novo synthesised short- and medium-chain 6-14-carbon fatty acid in milk fat (P?0,028). Camelina oil level had no effect on alphalinolenic acid content in milk fat (P>0.100). Increase of camelina oil level linearly increased trans fatty acids and CLA content in milk fat (P?0,008). Camelina oil supplementation did not affect neither the milk fat content of the final product of ruminal biohydrogenation of 18-carbon unsaturates stearic acid nor that of oleic acid (P>0,10). This is possibly due to biohydrogenation not proceeding to the end, ceasing to the last step before stearic acid. Milk fat trans-11 18:1 and cis-9, trans-11 CLA contents linearly increased at remarkably high levels when camelina oil level in the diet increased (P?0,008). This is possibly due to incomplete ruminal biohydrogenation of 18-carbon unsaturated fatty acids. Camelina oil supplement improved milk fat composition by decreasing saturated and increasing the trans-11 18:1 and cis-9,trans-11 CLA content in milk. However, giving camelina oil at high levels decreased silage and whole diet dry matter intake and affected milk production negatively.
  • Närkki, Sonja (Helsingin yliopisto, 2017)
    Leg weakness is one of the most common production diseases in pigs. Osteochondrosis is a joint disorder in which the endochondral ossification is disturbed. Leg weakness is mainly caused by osteochondrosis in pigs. Many factors like heredity and vitamin D status can affect the prevalence of osteochondrosis. This experiment was part of the international ProHealth project. The aim of this study was to investigate how two different vitamin D3 sources and feeding levels affect the prevalence of leg weakness in boars. Experiment design was 2 x 2 factorial. Commonly used vitamin D3 product (cholecalciferol) and Hy•D® product (25-hydroxycholecalciferol) were compared as vitamin D3 sources. Vitamin D3 feeding level 1 was in accordance of Finnish vitamin D3 recommendations and feeding level 2 was threefold with respect to the recommendations. Practical trial was carried out at Figen Oy’s research center in Längelmäki in 24.3. – 22.8.2016. Trial was conducted with 120 finishing boars. Average live weight of the boars at the end of the trial was 113 kg. Feed intake, daily weight gain, slaughter weight and bone strength were improved by greater feeding level of vitamin D3. Greater feeding level increased feed intake 6,13 kg of dry matter, slaughter weight 2,32 kg and bone strength 124,4 N during the trial. Daily weight gain increased 90,83 g/ d during the finishing period and 30,25 g/ d during the overall trial. Over 80 % of the pigs had excellent or good leg structure with only minor faults and their locomotion was fluent. There were no significant differences in leg structure or locomotion results between treatments. Pigs fed Hy•D® product with greater feeding level got double plasma 25-hydroxycholecalsiferol concentration when compared to pigs fed commonly used vitamin D3 product with greater feeding level. There were no significant treatment differences for 25-hydroxycholecalsiferol concentration in feeding level in accordance of the nutritional recommendations. There were no significant differences in plasma Ca, Pi or ALP concentrations between treatments either. Serious faults were observed more in distal humerus (elbow joint) compared to proximal humerus (shoulder joint) but there were no significant differences between treatments. In total of 11 pigs were removed from the treatments because of medical or tail biting reasons and in total of 50 pigs were medicated during the trial. Number of removed and medicated pigs divided evenly between treatments. According to this study the greater feeding level of vitamin D3 improved production. Feeding level of the vitamin D3 did not affect prevalence of the leg weakness or osteochondrosis. Source of the vitamin D3 did not affect production or prevalence of the leg weakness or osteochondrosis. Nutritional recommendations cover mainly the minimum need for nutrients and take no stand on optimizing production or health records. There is also some variation between research results. More research about the effects of vitamin D3 to prevalence of the leg weakness and the possibility to optimize production and health records with supplemental vitamin D3 is needed.
  • Mäntysaari, Nora (Helsingfors universitet, 2016)
    Tutkimuksen tavoitteena oli arvioida kuinka suuri osuus dobermannien välillä esiintyvästä eri käyttäytymisominaisuuksiin liittyvästä vaihtelusta on perinnöllistä sekä onko eri käyttäytymisominaisuuksien välillä perinnöllisiä yhteyksiä. Tutkimuksessa arvioitiin myös kyselytutkimuksen soveltuvuutta koirien käyttäytymisominaisuuksien periytymisen tutkimukseen. Tutkimuksessa käytetty aineisto kerättiin dobermannin omistajille suunnatun kyselytutkimuksen avulla. Aineisto sisälsi tiedot 153 dobermannista. Periytymisen analysointiin tarvittavat sukulaisuustiedot saatiin Suomen Kennelliitto ry:ltä rekisteröintidatana, joka sisälsi 14 404 dobermannin viralliset rekisterinumerot, isän ja emän rekisterinumerot, syntymäajan sekä tiedon koiran sukupuolesta. Fenotyyppiset käyttäytymismuuttujat analysoitiin useita kysymyksiä sisältävinä summamuuttujina. Periytymisasteet ja eri ominaisuuksien väliset geneettiset ja fenotyyppiset korrelaatiot arvioitiin AI-REML -menetelmällä käyttäen DMU-ohjelmaa. Tutkimus rajattiin kahdeksaan käyttäytymisominaisuuteen, joissa oli riittävän korkeat variaatiokertoimet periytymisen analysointia varten. Periytymisasteet olivat alhaisia ja keskivirheet olivat suuria. Tämän vuoksi periytymisasteista ei voida tehdä luotettavia johtopäätöksiä. Geneettisiä korrelaatioita yritettiin analysoida, mutta aineiston pienen koon vuoksi niiden estimointi ei ollut mahdollista. Fenotyyppisesti dobermannien aggressiivisuutta selittävät koiran sukupuoli sekä ympäristötekijöistä se, onko taloudessa yksi vai useampi koira. Dobermanneissa naaraat ilmentävät enemmän aggressiivisuutta kuin urokset. Useamman koiran taloudessa elävät ovat ainoita koiria aggressiivisempia, mitä voidaan selittää laumakäyttäytymisellä. Useissa roduissa urokset ovat naaraita aggressiivisempia. Tarvitaan lisätutkimusta, jotta voidaan selvittää, miksi dobermanneissa naaraat ovat uroksia aggressiivisempia. Tämä voisi liittyä emän suurempaan vaikutukseen käyttäytymismallien antajana koirien pentuaikana.
  • Saha, Sani (Helsingin yliopisto, 2021)
    Immunodeficiency in neonatal piglet is one of the major causes of pre-weaning mortality. Colostrum supply cytokines to newborn piglets, which play an important role in formation of their immune mechanism. This study aims to identify the relationship between sow’s blood and colostrum cytokines, and effect of resin acid-enriched composition (RAC) in sow’s blood and colostrum cytokines concentration. RAC is a plant extract of coniferous tree, and it is composed of resin acids and fatty acids which exert immunomodulatory function. Two experiments were conducted in two different farms where RAC was supplemented in the diet of late gestation sows, starting from one week before of expected farrowing date till farrowing. Multiplex immunoassay was used to determine the interferon alpha (IFN-α), interferon gamma (IFN-ɣ), interleukin 1 (IL-1), interleukin 10 (IL-10), interleukin 4 (IL-4), interleukin 6 (IL-6), interleukin 8 (IL-8), tumor necrosis factor alpha (TNF-α) and interleukin 12p40 (IL-12p40) cytokines concentration in blood and colostrum of sow collected within 2 hours of the birth of first piglets. There were significant (p < 0.05) positive correlations between sow’s blood and colostrum cytokines concentration. RAC-fed sows had significantly (p < 0.05) higher level of IL-1 and showed positive tendency (p = 0.1) to increase TNF-α cytokine level in blood. Colostrum level of IFN- ɣ, IL-1 and IL-12p40 had also positive tendency (p = 0.1) to rise in RAC supplemented sow. On this basis, it is proposed that application of RAC in late gestation sow diet enhance the cytokine production in sow’s blood, and blood cytokines levels are positively correlated with colostrum cytokines.
  • Fant, Petra (Helsingin yliopisto, 2017)
    The aim of this master’s thesis was to investigate the potential of reducing enteric methane production from dairy cows by replacing barley grain with oat grain on a grass silage-based diet. The effects of grain species on in vitro methane production, digestibility, pH and volatile fatty acid (VFA) production pattern were investigated and a regression analysis was performed to entangle possible methane mitigating mechanisms of oats. The study was conducted in the laboratory at the Department of Agricultural Research for Northern Sweden in the autumn of 2016. An in vitro gas production system was applied, consisting of 16 diets with two replicates, four blanks and three runs, each with a three-day incubation time. The feed material consisted of eight varieties of barley, eight varieties of oats and timothy silage incubated at a grain/silage ratio of 1:1 on a dry matter basis. Rumen fluid was collected from two cannulated Nordic Red dairy cows after morning feeding. Gas sampling was performed at 2, 4, 8, 12, 24, 32 and 48 hours of incubation, meanwhile VFA-sampling, pH-measurements and sampling of incubation residues were performed at 48 hours. Methane production was estimated as predicted in vivo methane production and stoichiometrically predicted methane production. The in vitro digestibility was determined as true dry matter digestibility (TDMD). Content of indigestible neutral detergent fiber (iNDF) was determined by applying a 12-day in situ incubation in two Ayrshire dairy cows. Oats had a higher content of crude protein, neutral detergent fiber (NDF), iNDF and fat compared to barley, whereas barley had a higher content of starch. Replacing barley with oats decreased predicted in vivo methane production by 9% and stoichiometrically predicted methane production by 11%. Variety within grain was not found to have any significant effect on methane production. True DM digestibility and total VFA production were lower in oat-based diets compared to barley-based diets. No significant differences were observed between the diets considering VFA molar proportions. The pH was lower in barley-based diets compared to oat-based diets. True DM digestibility was the best predictor of methane production. Among grain composition parameters, iNDF content was the best predictor of methane production, followed by NDF. Crude fat content also predicted methane production relatively well. Based on the results of this experiment, it can be concluded that replacing barley grain with oat grain in the diet of dairy cows has a potential to lower methane production predicted in vitro. Furthermore, the methane mitigating effect observed in this experiment is at least partly due to the higher fat content and lower digestibility of oats compared to barley.
  • Gao, Jianguo (Helsingfors universitet, 2017)
    Obesity and insulin resistance (IR) are key factors lead to equine metabolic syndrome and laminitis. Diet may play an important role in eliciting obesity by affecting insulin dynamics. Insulin-pathway signaling and mTORC1 genes may contribute to incred IR. The first objective of this study was to find and validate internal control genes for quantitative PCR method for adipose tissues in Finnhorse mares. The second aim was to quantitate the expression of mTORC1 and insulin-pathway associated genes after pasture season in two different treatment groups of Finnhorse mares and compare gene expression differences between treatment groups. In addition, gene expression differences were compared between two different adipose tissues. Twenty-two mares were equally divided into eleven equal pairs, the two mares of each group were randomly grazed either on cultivated high-yielding pasture (CG) or on semi-natural grassland (NG) from the end of May to the beginning of September. Eight pairs of Finnhorse mares were selected for gene expression profiling. Subcutaneous adipose tissue (SAT) samples were collected from two groups of Finnhorse mares after pasture season. Gene expression of neck and tailhead SAT were determined with quantitative Real-Time PCR method (qPCR). The selected internal control genes were actin beta (ACTB), glucuronidase beta (GUSB) and mitochondrial ribosomal protein L39 (MRPL39). Candidate genes were mechanistic target of rapamycin (MTOR), sterol regulatory element binding transcription factor 1 (SREBF1), sterol regulatory element binding transcription factor 2 (SREBF2), TBC1 domain family member 7 (TBC1D7), leptin (LEP), glucose transporter type 4 (GLUT4), monocyte chemoattractant protein-1 (MCP-1), retinol binding protein 4 (RBP4), tuberous sclerosis 1 (TSC1), tuberous sclerosis 2 (TSC2). There were no distinct gene expression differences between NG and CG groups in both neck and tailhead SAT. However, RBP4 had significantly (P=0.035) higher and GLUT4 had a trend (P=0.064) to higher mRNA expression in CG group in neck SAT. TSC1 had a trend (P=0.071) of higher expression in CG group in tailhead SAT. Gene expression differences were observed between tailhead and neck SAT. SREBF1 and GLUT4 had significantly (P=0.007 and P=0.026, respectively) higher expression levels in tailhead SAT compared to neck SAT. RBP4 had a trend (P=0.066) to higher expression in neck SAT compared to tailhead SAT. Minor differences in gene expression between NG and CG groups indicate that pasture-associated fat depositionmaynotconsiderably affect expressionof insulin-pathway and mTORC1 genes associated to obesity and IR in studied subcutaneous adipose tissues. These results also provide additional evidence to our hypothesis that fattening resulting on unrestricted grazing on cultivated high-yielding pasture does not increase the risk of metabolic diseases in Finnhorse mares when they have normal body condition at the beginning of the grazing season.
  • Norrback, Josefin (Helsingin yliopisto, 2021)
    Gastrointestinal parasites (GIP) and resistance against anthelmintics are and will be one of the biggest challenges for the sheep husbandry. Research within this area is very important today and it is important to figure out new methods to identify individuals with high burden of parasites and to treat just these individuals. The aim of the study was to investigate the effects of GIP on activity patterns and weight gain in first season grazing lambs. A sub-study accompanied with the aim was to see if the manually made behavioural observations supported data from the sensors. The study took place at the Swedish University of Agricultural Sciences (SLU) during the summer of 2019. The study was divided into two experimental periods. In the first experimental period 30 ewes and their 60 twins were used. Of those, 58 lambs continued to the second experimental period. Two groups were dewormed with ivermectin (high group) and two groups were untreated (low group). All lambs were fitted with sensors, and the data was downloaded once a week, at the same time with the weighting of animals. Individual faecal samples were collected four times. The pasture where the lambs grazed was a first-year pasture. Sward height was measured, and herbage samples were collected three times during the experiment. Herbage samples were analysed for nutritional and mineral content. The manual behavioural observations were made two days a week during five weeks. The high group had higher weight (28.9 kg) in the beginning of the experiment, compared with the low group (28.1 kg). On the contrary, the high group had lower weight (42.7 kg), compared with the low group (44.8 kg) at the end of the experiment. According to data from the sensors there were differences in lying time and motion index (MI) during the 7-10 first days after weaning. The low group laid down more, compared with the high group. The low group had higher MI compared with the high group. During the whole experiment there were no differences in lying time, lying bouts or MI. The manual behavioural observations showed that “standing still” had significantly more registrations for the high group compared to the low group. It is possible to see differences in behaviour with sensors at an early stage of parasitic infection, even with low levels of parasites. This means, that there is a potential to use automatic behaviour observations as a diagnostics tool during infection of GIP.
  • von Konow, Helena (Helsingin yliopisto, 2020)
    The feeding behavior of beef cattle is complex and varies depending on different factors such as the nutritive value of the feed, the individual and the environment. By optimizing and improving the feed efficiency, the economics of the production increases. Knowledge of feed intake and feeding behavior can be used to optimize the production and the health and well-being of the animals. The aim of this study was to determine how the cut of grass silage affects the feeding behaviour of bulls and thereby how to optimize the use of silage for animal growth. Simmental bulls got three different silage cuts. The first cut was taken on 25th June (ES1), the second cut on 11th August (ES2) and the third cut on 3rd October (ES3). The feeding was given as total mixed ration (TMR) ad libitum. Each TMR group had 15 bulls (TES1, TES2, TES3). Each TMR group had a diet based on grass silage (550 g/kg DM), the only difference being if the silage was of the first, second or third cut. That way the nutritive value differed among the TMR. Individual information about duration, when, and how much the bulls ate was recorded by the Growsafesystem. The average for the bulls in the feeding groups was calculated based on the individual data. This Master´s thesis researched feed intake, feeding frequency, time for head down, time for feed intake and feed intake rate during one month when the body weight of the bulls was on average 546 kg in the beginning and 607 kg at the end of the study. The dry matter for the three cuts was 201 g/kg for ES1, 298 g/kg for ES2 and 354 g/kg for ES3. D-value was 719, 685 and 739 g/kg DM, respectively. The bulls that ate ES3 had higher dry matter intake (12,1 kg DM/d) and eating rate (94,9 g DM/min) than the two other groups. The bulls that ate ES1 and ES2 had the same feed utilization, which was better than the feed utuilization of the bulls that were given ES3. Feeding frequency was about 15 times per day for all groups. The second cut had the lowest digestibility and the bulls eating the second cut had the longest feed intake time. The bulls that ate the third cut had the highest dry matter intake, but the lowest feed utilization. The study indicates that the D-value of the grass silage used in TMR has a clear impact on dry matter intake of the TMR and eating time and eating rate of the animal.
  • Mikkola, Aino (Helsingin yliopisto, 2019)
    Before parturition the wild boar uses plant material to build a nest, which provides the piglets shelter and keeps them warm. Despite domestication, this behaviour has remained as an important part of maternal behaviour in the domestic pig. Nest building behaviour has a big impact on modern pig production, because the possibilities to practise this behaviour affect the sow and the piglets in various ways. In this study, we investigated nest building behaviour in a group farrowing system. We also studied how nest building behaviour is associated to the sow’s physiology and performance. A total of 31 farrowings of 23 group-housed sows were investigated. The nest building behavior and the location of the sows were monitored continuosly starting 24 hours prior to farrowing. When the sow farrowed, blood and colostrum samples were collected to assess the concentrations of progesterone and immunoglobulins. Also the duration of farrowing, the yield of colostrum and the piglets’ growth, colostrum intake and mortality were observed. The sows had excellent conditions for nest building, because they could move freely in their own group farrowing department and had access to large amounts of straw to use as a nest building material. The total duration of nest building behaviour and the way it was distributed varied greatly between sows. Nest building behaviour started on average 23 h 7 min before farrowing and ceased approximately 18 min before farrowing. The mean total duration of nest building behaviour during 24 hours before farrowing was 4 h 29 min. Most of the nest building behaviour, 3 h 32 min, occurred 12–0 h before farrowing and the peak was seen 6–4 hours prior to farrowing. Younger sows started nest building behaviour earlier and spent less time in the pens than older sows. Starting nest building behaviour earlier correlated with a shorter duration of farrowing and, to a lesser extent, with a smaller number of stillborn piglets. Abundant nest building during 24–12 hours before farrowing tended to correlate with lower piglet mortality in the age of 1–3 days. Opposite to our assumptions, abundant nest building during 12–0 hours before farrowing correlated with poorer piglet growth. Nest building behaviour wasn’t related to colostrum yield and intake or the concentrations of progesterone and immunoglobulins. In conclusion, the total duration of nest building behaviour was greater in group farrowing system than in previously studied systems (farrowing crate, loose farrowing pen). Especially the early start of nest building had a positive effect on sows’ performance.
  • Jämsä, Titta (Helsingin yliopisto, 2020)
    Tämän tutkielman tavoitteena oli selvittää suomalaisen emolehmätuotannon tuottavuutta kuvaavia tunnuslukuja. Emolehmätuotantoa tarvitaan, koska lypsylehmien määrä pienenee ja näin ollen myös lihantuotantoon käytettävissä olevien maitorotuisten vasikoiden määrä. Emolehmien määrä on noussut Suomessa lähes 20 % kymmenen viime vuoden aikana. Emolehmän tärkein tuotos on elävä vasikka, joka saadaan vieroitettua menestyksekkäästi ja riittävän isossa vieroituspainossa suhteessa emon painoon. Naudanlihantuotannon hiilijalanjälkeen on viime vuosina kiinnitetty yhä enemmän huomiota ja emolehmiin perustuvan lihantuotannon tuottavuutta parantamalla voidaan pienentää myös kasvihuonekaasupäästöjä. Emolehmätuotannon tuottavuutta kuvaavia tunnuslukuja ovat hiehojen poikimaikä, poikimaväli, poikimaprosentti, vasikkakuolleisuus, elinikäistuotos ja poistoikä. Nämä analysoitiin Ruokaviraston nautarekisterin tietojärjestelmistä saatavasta datasta vuosilta 1995-2014. Aineiston tilastollinen analysointi toteutettiin SAS-ohjelmalla. Keskimääräisten tunnuslukujen lisäksi analysoitiin rotukohtaiset erot sekä emolehmätilojen parhaimman ja heikoimman neljänneksen väliset erot. Emolehmähiehot poikivat keskimäärin 27,5 kuukauden iässä. Poikimaväli oli keskimäärin 378 päivää. Angus-rodun hiehojen poikimaikä oli matalin ja lehmien poikimaväli lyhyin lähes koko analysointijakson ajan. Limousinen poikimaikä oli korkein ja poikimaväli pisin. Parhaan neljänneksen tiloilla hiehot poikivat keskimäärin 22 kuukauden iässä ja heikoimman neljänneksen tiloilla yli 42 kuukauden iässä. Poikimavälin keskiarvo parhaan neljänneksen tiloilla oli 364 päivää ja heikoimman neljänneksen tiloilla 400 päivää. Poikimaprosentti oli keskimäärin lähes 86 prosenttia. Anguksen poikimaprosentti oli korkein koko analysointijakson ajan. Parhaan neljänneksen karjoissa poikimaprosentti oli keskimäärin 96 % ja heikoimman neljänneksen karjoissa 73 %. Vasikkakuolleisuus oli keskimäärin 6,2 %. Sonnivasikoiden kuolleisuus oli lähes 2 %-yksikköä suurempi kuin lehmävasikoiden. Ensikoilla vasikkakuolleisuus oli suurempi kuin vanhemmilla lehmillä. Vasikkakuolleisuus oli suurin charolais-rodulla. Emolehmän elinikäistuotos oli keskimäärin 4,4 tuotettua vasikkaa. Elinikäistuotos oli paras anguksella ja limousinella ja heikoin simmentalilla. Poistoiän keskiarvo oli 6,8 vuotta. Korkein poistoikä oli limousinella ja matalin simmentalilla. Tilakohtaiset vaihtelut tuotantotuloksissa olivat suuria. Tuotannon tulisi olla riittävän tehokasta, jotta se olisi ympäristöllisesti kestävää ja emolehmätuotannon hiilijalanjälkeä saataisiin entisestään pienennettyä.
  • Pellinen, Paula (Helsingin yliopisto, 2022)
    Harvesting pure red clover as silage gives new possibilities to optimize forage feeding of cattle for different animal groups and compromises between plants during cultivating, harvesting, wilting or ensiling are not needed as with mixed swards. This pilot scale preservation study was made as part of Opti-Palko project and the aim was to resolve best practices to ensile pure red clover. Si-lages was made from second harvest pure stands of Selma red-clover and Nuutti timothy and 50% / 50% mixture of those. The crops were ensiled immediately after harvesting and after wilting in dryer. There were five additive treatments comprising of three different acid-based products (formic acid based AIV 2 Plus Na (AIV2), AIV Via (VIA) which includes high proportion of propionic acid, un-corrosive acid mixture buffered with sodium formate (AIVB)) and heterofermentative lactic acid bacteria inoculant Josilac Combi (LAB) and control (KON). Control silages were untreated. Silages were ensilaged in vacuum bags which were opened after a storage period of 12 weeks. After opening chemical composition, microbial quality and aerobic stability were analyzed. The effect of preservatives, dry matter content (DM) (low DM: 114-135 g / kg, high DM ka: 252-305 g / kg) and the proportion of red clover (100-50-0 %) on silage quality and aerobic stability was studied. Sta-tistically significant results between treatments were observed. Wilting increased the quality of silages and well-fermented pure red clover silages were achieved when acid-based additives were used. For example, the concentration of volatile fatty acids was significantly lower than in KON and LAB treatments. In low DM KON and LAB silages very high in acetic acid which didn’t improve aerobic stability in this study but resulted in aerobic unstable silages after around 60 hours. With acid-based additive treated red clover silages, the ratio of ammonia N to total N increased with increasing DM content, which was opposite when compared to other plant materials. This might be due to polyphenoloxidase enzyme (PPO) in red clover, which inhibits the degradation of pro-tein. This study resulted that using silage preservatives is necessary in low DM silages. pH and the content of acetic acid increased with portion of red clover, but lactic acid, propionic acid and etha-nol decreased statistically significantly. AIVB treated silages resulted with same level of quality than other acid-based additive treaded red clover silages, even it was highly buffered to be non-corrosive. The results of this study are useful when choosing preservatives for red clover and gives a new perspective for harvesting silage from pure stands.
  • Kuusisto, Katja (Helsingin yliopisto, 2015)
    The aim of this study was to investigate the ensilability of field pea and faba bean bi-crop with spring wheat when ensiled as whole-crop with different additives. In this study we attempted to clarify use of pea – wheat and faba bean – wheat bi-crop in organic farming. Forages was sown at 4th to 7th of June in Luonnonvarakeskus (Luke) Ruukki and forages were ensiled in laboratory silos at 26th of August 2013. Silages were ensiled without additive (PR), with formic acid (MH) and with two different heterofermentative inoculants (B1 and B2) as additive. Each treatment were made three replicants. Silos were opened after 106 ensiling days at 9th of December in Luke Jokioinen. Chemical and microbiological compositions were determined from samples of herbage. From silage samples were analysed fermentation quality, microbiological composition and aerobic stability. All samples were wet. Faba bean – wheat bi-crop was a little wetter than field pea – wheat bi-crop. Field pea – wheat forage contained more crude protein, water-soluble carbohydrates, starch and had stronger buffering capacity than faba bean – wheat. Faba bean – wheat bi-crop contained more neutral-detergent fiber (NDF) and had also lower digestibility. Both of crops were easy to ensile and growth stage were different between crops. All samples included lot of micro-organism especially yeast, moulds and epiphytic lactic acid bacteria. Field pea – wheat silage contained more fermentation products than faba bean – wheat silage. MH- treated silages had better fermentation quality than other silages although there were higher pH values in MH silages. MH silages contained less lactic and acetic acids and ammonium nitro-gen. Inoculant treated silages mostly did not differ from PR treatment in fermentation parameters. That is probably due to high epiphytic lactic acid bacteria account in herbage. Silages contained only a little butyric acid. Microbiological quality of silages predisposed them to aerobic deteriora-tion because there were considerable high amounts of yeasts and moulds. Field pea –wheat silages were more aerobically stable than faba bean - wheat. This is probably due to higher concentration of fermentation acids in field pea-wheat silages. MH treatment was the most aerobically stable of field pea – wheat silages. B2 treatment was the most stable of field pea – wheat silages. Mixed ration of these silages were more prone to aerobically deterioration than silages.
  • Salakka, Anna (Helsingin yliopisto, 2019)
    The objective of the experiment was to study how different additives effect on fermentation quality and aerobic stability of maize silage. Three additives and two maturity stages were used in the labo-ratory scale experiment. Maize silage preserved with different additives was compared to control with no additives. The maize (Pioneer, P7326) used in the study was cultivated at the research farm of the Univer-sity of Helsinki in Viikki on growing season 2018. First silages were made on 21st of August 2018 and after 50 days the second silages were made on 10th of October 2018 92 and 142 days after sowing. The treatments were 1) control 2) formic acid 3) mixture of formic acid, sodium formate, propionic acid and sorbic acid 4) mixture of sodium benzoate and potassium sorbate. Chemical com-positon and buffering capacity of the pre-ensiling maize were determined. Fermentation quality of the silages was also determined. In addition aerobic stability of silages was determined by monitoring the temperature change of silages for 12 days with data loggers. Composition of the maize varied by the maturity stage. Dry matter content in the first maturity stage was 211 g/kg and in the second it was 327 g/kg. Content of water soluble carbohydrates (su-gars) was higher in the first maturity stage. Starch content increased in the second maturity stage considerably. Feed preservation succeeded mainly well and pH of silages in both maturity stages were below four. Sugar content of silages treated with formic acid and mixture of acids was very high in the first maturity stage. Lactic acid content of the control and Na-benzoate and K-sorbate silages was higher than other treatments. Na-benzoate and K-sorbate silage in late maturity stage had noticeably higher acetic acid content. In the silages, there were mainly low content of volatile fatty acids (VFA) and butyric acid was not observed. The silages contained lactic acid as well as acetic acid and only low amount of propionic acid. Silages were aerobically stable except silages of two formic acid silos in the first maturity stage and control silos in the second maturity stage. Temperature started to rise in formic acid silages after 10 days and in control silages after five days from exposure to air. Other silages were aerobically stable during the testing period. The maturity stage affected maize composition and thus ensiling characteristics. Composition differences of maize in different maturity stages cause different challenges in terms of preservation. Different preservation treatments affected the intensity of lactic acid fermentation and silage sugar content and the differences were higher in the first maturity stage. Intensity differences of fermenta-tion and differences in acetic acid content may affect aerobic stability. The use of additives tended to improve aerobic stability of the silages at the late stage of maturity.
  • Sarviaho, Katri (Helsingin yliopisto, 2019)
    The aim of this study was to estimate the genetic parameters of elk hunting traits in Jämthunds. There were nineteen traits under consideration. Heritabilities, repeatabilities, and genetic and phenotypic correlations were estimated for the traits. Also, coefficient of inbreeding and genetic trends were estimated. The data consisted of results from official elk hunting trials collected by Suomen Harmaahirvikoirajärjestö ry in 2012-2016. There were 46 221 results, from which 23 335 of Jämthunds. The pedigree data was provided by The Finnish Kennel Club and it included 31 544 Jämthunds. Significance of the fixed effects was estimated using F-test in analysis of variance with RStudio 1.0.136. The pedigree was pruned with RelaX2 1.54-pedigree analysis programme. Variance components were estimated with DMU-package using AI-REML-approach. The estimated heritabilities were low and varied between 0.00 and 0.047. The highest heritability was obtained for search and the lowest for obedience during work. Genetic correlations varied from -0.25 to 0.98, and the strongest were estimated for most of the bark related traits. The genetic trend has been positive in all traits, except for obedience traits. The coefficient of inbreeding for dogs born in 2016 was approximately 7.03 %, and the coefficient of inbreeding has decreased 0.26 % in the last decade. There are multiple non-genetic factors that affect the traits, and the data is based on subjectively evaluated variables. It is possible to improve genetic evaluation by collecting more information on trial conditions, by using the whole scale of points during the evaluation, and by making more objective evaluations of the traits. The estimated breeding values of the important traits can be used in selection of the parent of the next generation.