Browsing by Subject "IN-SITU HYBRIDIZATION"

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  • Wolf, Maija; Korja, Miikka; Karhu, Ritva; Edgren, Henrik; Kilpinen, Sami; Ojala, Kalle; Mousses, Spyro; Kallioniemi, Anne; Haapasalo, Hannu (2010)
    Neuroblastoma has successfully served as a model system for the identification of neuroectoderm-derived oncogenes. However, in spite of various efforts, only a few clinically useful prognostic markers have been found. Here, we present a framework, which integrates DNA, RNA and tissue data to identify and prioritize genetic events that represent clinically relevant new therapeutic targets and prognostic biomarkers for neuroblastoma.
  • Giaretta, Paula R.; Suchodolski, Jan S.; Jergens, Albert E.; Steiner, Jorg M.; Lidbury, Jonathan A.; Cook, Audrey K.; Hanifeh, Mohsen; Spillmann, Thomas; Kilpinen, Susanne; Syrja, Pernilla; Rech, Raquel R. (2020)
    The intestinal microbiota is believed to play a role in the pathogenesis of inflammatory bowel disease in humans and chronic inflammatory enteropathy (CIE) in dogs. While most previous studies have described the gut microbiota using sequencing methods, it is fundamental to assess the spatial distribution of the bacteria for a better understanding of their relationship with the host. The microbiota in the colonic mucosa of 22 dogs with CIE and 11 control dogs was investigated using fluorescence in situ hybridization (FISH) with a universal eubacterial probe (EUB338) and specific probes for select bacterial groups. The number of total bacteria labeled with EUB338 probe was lower within the colonic crypts of dogs with CIE compared to controls. Helicobacter spp. and Akkermansia spp. were decreased on the colonic surface and in the crypts of dogs with CIE. Dogs with CIE had increased number of Escherichia coli/Shigella spp. on the colonic surface and within the crypts compared to control dogs. In conclusion, the bacterial microbiota in the colonic mucosa differed between dogs with and without CIE, with depletion of the crypt bacteria in dogs with CIE. The crypt bacterial species that was intimately associated with the host mucosa in control dogs was composed mainly of Helicobacter spp.
  • Kettunen, Eeva; Savukoski, Sauli; Salmenkivi, Kaisa; Böhling, Tom; Vanhala, Esa; Kuosma, Eeva; Anttila, Sisko; Wolff, Henrik (2019)
    BackgroundDeletion of the CDKN2A locus is centrally involved in the development of several malignancies. In malignant pleural mesothelioma (MPM), it is one of the most frequently reported genomic alteration. MPM is strongly associated with a patients' asbestos exposure. However, the status of CDKN2A and the expression of the corresponding protein, p16, in relation to MPM patient's asbestos exposure is poorly known. Copy number alterations in 2p16, 9q33.1 and 19p13 have earlier been shown to accumulate in lung cancer in relation to asbestos exposure but their status in MPM is unclear.MethodsWe studied DNA copy numbers for CDKN2A using fluorescence in situ hybridization (FISH) and p16 expression by immunohistochemistry (IHC) in 92 MPM patients, 75 of which with known asbestos exposure status. We also studied, in MPM, copy number alterations in 2p16, 9q33.1 and 19p13 by FISH.ResultsWe were unable to detect an association between p16 expression and pulmonary asbestos fiber count in MPM tumor cells. However, significantly more MPM patients with high pulmonary asbestos fiber count (>1 million fibers per gram [f/g]) had stromal p16 immunoreactivity than MPM of patients with low exposure ( 0.5 million f/g) (51.4% vs 16.7%; p=0.035, Chi-Square). We found that an abnormal copy number of CDKN2A in MPM tumor cells associated with a high pulmonary asbestos fiber count (p=0.044, Fisher's Exact test, two-tailed). In contrast to our earlier findings in asbestos associated lung cancer, DNA copy number changes in 2p16, 9q33 and 19p13 were not frequent in MPM although single cases with variable copy numbers on those regions were seen.ConclusionsWe found two instances where the gene locus CDKN2A or its corresponding protein expression, is associated with high asbestos exposure levels. This suggests that there may be biological differences between the mesotheliomas with high pulmonary asbestos fiber count and those with low fiber count.
  • Tran, Quoc Ty; Jatsenko, Tatjana; Poolamets, Olev; Tsuiko, Olga; Lubenets, Dmitri; Reimand, Tiia; Punab, Margus; Peters, Maire; Salumets, Andres (2019)
    PurposeThe purpose of this study was to develop a feasible approach for single sperm isolation and chromosome analysis by next-generation sequencing (NGS).MethodsSingle sperm cells were isolated from semen samples of normozoospermic male and an infertile reciprocal translocation (RcT) carrier with the 46,XY,t(7;13)(p12;q12.1) karyotype using the optimized fluorescence-activated cell sorting (FACS) technique. Genome profiling was performed using NGS.ResultsFollowing whole-genome amplification, NGS,and quality control, the final chromosome analysis was performed on 31 and 6 single cell samples derived from the RcT carrier and normozoospermic male, respectively. All sperm cells from normozoospermic male showed a normal haploid 23-chromosome profile. For the RcT carrier, the sequencing data revealed that 64.5% of sperm cells harbored different variants of chromosome aberrations, involving deletion of 7p or 7q, duplication of 7p, and duplication of 13q, which is concordant with the expected chromosome segregation patterns observed in balanced translocation carriers. In one sample, a duplication of 9q was also detected.ConclusionsWe optimized FACS protocol for simple and efficient isolation of single human sperm cells that subsequently enabled a successful genome-wide chromosome profiling and identification of segmental aneuploidies from these individual cells, following NGS analysis. This approach may be useful for analyzing semen samples of infertile men or chromosomal aberration carriers to facilitate the reproductive risk assessment.
  • Zamora, Juan Carlos; Svensson, Mans; Kirschner, Roland; Olariaga, Ibai; Ryman, Svengunnar; Alberto Parra, Luis; Geml, Jozsef; Rosling, Anna; Adamcik, Slavomir; Ahti, Teuvo; Aime, M. Catherine; Ainsworth, A. Martyn; Albert, Laszlo; Alberto, Edgardo; Garcia, Alberto Altes; Ageev, Dmitry; Agerer, Reinhard; Aguirre-Hudson, Begona; Ammirati, Joe; Andersson, Harry; Angelini, Claudio; Antonin, Vladimir; Aoki, Takayuki; Aptroot, Andre; Argaud, Didier; Sosa, Blanca Imelda Arguello; Aronsen, Arne; Arup, Ulf; Asgari, Bita; Assyov, Boris; Atienza, Violeta; Bandini, Ditte; Baptista-Ferreira, Joao Luis; Baral, Hans-Otto; Baroni, Tim; Barreto, Robert Weingart; Baker, Henry; Bell, Ann; Bellanger, Jean-Michel; Bellu, Francesco; Bemmann, Martin; Bendiksby, Mika; Bendiksen, Egil; Bendiksen, Katriina; Benedek, Lajos; Beresova-Guttova, Anna; Berger, Franz; Berndt, Reinhard; Bernicchia, Annarosa; Biketova, Alona Yu; Bizio, Enrico; Bjork, Curtis; Boekhout, Teun; Boertmann, David; Bohning, Tanja; Boittin, Florent; Boluda, Carlos G.; Boomsluiter, Menno W.; Borovicka, Jan; Brandrud, Tor Erik; Braun, Uwe; Brodo, Irwin; Bulyonkova, Tatiana; Burdsall, Harold H.; Buyck, Bart; Burgaz, Ana Rosa; Calatayud, Vicent; Callac, Philippe; Campo, Emanuele; Candusso, Massimo; Capoen, Brigitte; Carbo, Joaquim; Carbone, Matteo; Castaneda-Ruiz, Rafael F.; Castellano, Michael A.; Chen, Jie; Clerc, Philippe; Consiglio, Giovanni; Corriol, Gilles; Courtecuisse, Regis; Crespo, Ana; Cripps, Cathy; Crous, Pedro W.; da Silva, Gladstone Alves; da Silva, Meiriele; Dam, Marjo; Dam, Nico; Dammrich, Frank; Das, Kanad; Davies, Linda; De Crop, Eske; De Kesel, Andre; De Lange, Ruben; Bonzi, Barbara De Madrignac; dela Cruz, Thomas Edison E.; Delgat, Lynn; Demoulin, Vincent; Desjardin, Dennis E.; Diederich, Paul; Dima, Balint; Dios, Maria Martha; Divakar, Pradeep Kumar; Douanla-Meli, Clovis; Douglas, Brian; Drechsler-Santos, Elisandro Ricardo; Dyer, Paul S.; Eberhardt, Ursula; Ertz, Damien; Esteve-Raventos, Fernando; Salazar, Javier Angel Etayo; Evenson, Vera; Eyssartier, Guillaume; Farkas, Edit; Favre, Alain; Fedosova, Anna G.; Filippa, Mario; Finy, Peter; Flakus, Adam; Fos, Simon; Fournier, Jacques; Fraiture, Andre; Franchi, Paolo; Molano, Ana Esperanza Franco; Friebes, Gernot; Frisch, Andreas; Fryday, Alan; Furci, Giuliana; Marquez, Ricardo Galan; Garbelotto, Matteo; Garcia-Martin, Joaquina Maria; Otalora, Monica A. Garcia; Sanchez, Dania Garcia; Gardiennet, Alain; Garnica, Sigisfredo; Benavent, Isaac Garrido; Gates, Genevieve; Gerlach, Alice da Cruz Lima; Ghobad-Nejhad, Masoomeh; Gibertoni, Tatiana B.; Grebenc, Tine; Greilhuber, Irmgard; Grishkan, Bella; Groenewald, Johannes Z.; Grube, Martin; Gruhn, Gerald; Gueidan, Cecile; Gulden, Gro; Gusmao, Luis F. P.; Hafellner, Josef; Hairaud, Michel; Halama, Marek; Hallenberg, Nils; Halling, Roy E.; Hansen, Karen; Harder, Christoffer Bugge; Heilmann-Clausen, Jacob; Helleman, Stip; Henriot, Alain; Hernandez-Restrepo, Margarita; Herve, Raphael; Hobart, Caroline; Hoffmeister, Mascha; Hoiland, Klaus; Holec, Jan; Holien, Hakon; Hughes, Karen; Hubka, Vit; Huhtinen, Seppo; Ivancevic, Boris; Jagers, Marian; Jaklitsch, Walter; Jansen, AnnaElise; Jayawardena, Ruvishika S.; Jeppesen, Thomas Stjernegaard; Jeppson, Mikael; Johnston, Peter; Jorgensen, Per Magnus; Karnefelt, Ingvar; Kalinina, Liudmila B.; Kantvilas, Gintaras; Karadelev, Mitko; Kasuya, Taiga; Kautmanova, Ivona; Kerrigan, Richard W.; Kirchmair, Martin; Kiyashko, Anna; Knapp, Daniel G.; Knudsen, Henning; Knudsen, Kerry; Knutsson, Tommy; Kolarik, Miroslav; Koljalg, Urmas; Kosuthova, Alica; Koszka, Attila; Kotiranta, Heikki; Kotkova, Vera; Koukol, Ondrej; Kout, Jiri; Kovacs, Gabor M.; Kriz, Martin; Kruys, Asa; Kudera, Viktor; Kudzma, Linas; Kuhar, Francisco; Kukwa, Martin; Kumar, T. K. Arun; Kunca, Vladimir; Kusan, Ivana; Kuyper, Thomas W.; Lado, Carlos; Laessoe, Thomas; Laine, Patrice; Langer, Ewald; Larsson, Ellen; Larsson, Karl-Henrik; Laursen, Gary; Lechat, Christian; Lee, Serena; Lendemer, James C.; Levin, Laura; Lindemann, Uwe; Lindstrom, Hakan; Liu, Xingzhong; Hernandez, Regulo Carlos Llarena; Llop, Esteve; Locsmandi, Csaba; Lodge, Deborah Jean; Loizides, Michael; Lokos, Laszlo; Luangsa-ard, Jennifer; Luderitz, Matthias; Lumbsch, Thorsten; Lutz, Matthias; Mahoney, Dan; Malysheva, Ekaterina; Malysheva, Vera; Manimohan, Patinjareveettil; Mann-Felix, Yasmina; Marques, Guilhermina; Martinez-Gil, Ruben; Marson, Guy; Mata, Gerardo; Matheny, P. Brandon; Mathiassen, Geir Harald; Matocec, Neven; Mayrhofer, Helmut; Mehrabi, Mehdi; Melo, Ireneia; Mesic, Armin; Methven, Andrew S.; Miettinen, Otto; Romero, Ana M. Millanes; Miller, Andrew N.; Mitchell, James K.; Moberg, Roland; Moreau, Pierre-Arthur; Moreno, Gabriel; Morozova, Olga; Morte, Asuncion; Muggia, Lucia; Gonzalez, Guillermo Munoz; Myllys, Leena; Nagy, Istvan; Nagy, Laszlo G.; Neves, Maria Alice; Niemela, Tuomo; Nimis, Pier Luigi; Niveiro, Nicolas; Noordeloos, Machiel E.; Nordin, Anders; Noumeur, Sara Raouia; Novozhilov, Yuri; Nuytinck, Jorinde; Ohenoja, Esteri; Fiuza, Patricia Oliveira; Orange, Alan; Ordynets, Alexander; Ortiz-Santana, Beatriz; Pacheco, Leticia; Pal-Fam, Ferenc; Palacio, Melissa; Palice, Zdenek; Papp, Viktor; Partel, Kadri; Pawlowska, Julia; Paz, Aurelia; Peintner, Ursula; Pennycook, Shaun; Pereira, Olinto Liparini; Daniels, Pablo Perez; Capella, Miguel A. Perez-De-Gregorio; del Amo, Carlos Manuel Perez; Gorjon, Sergio Perez; Perez-Ortega, Sergio; Perez-Vargas, Israel; Perry, Brian A.; Petersen, Jens H.; Petersen, Ronald H.; Pfister, Donald H.; Phukhamsakda, Chayanard; Piatek, Marcin; Piepenbring, Meike; Pino-Bodas, Raquel; Esquivel, Juan Pablo Pinzon; Pirot, Paul; Popov, Eugene S.; Popoff, Orlando; Alvaro, Maria Prieto; Printzen, Christian; Psurtseva, Nadezhda; Purahong, Witoon; Quijada, Luis; Rambold, Gerhard; Ramirez, Natalia A.; Raja, Huzefa; Raspe, Olivier; Raymundo, Tania; Reblova, Martina; Rebriev, Yury A.; Garcia, Juan de Dios Reyes; Ripoll, Miguel Angel Ribes; Richard, Franck; Richardson, Mike J.; Rico, Victor J.; Robledo, Gerardo Lucio; Barbosa, Flavia Rodrigues; Rodriguez-Caycedo, Cristina; Rodriguez-Flakus, Pamela; Ronikier, Anna; Casas, Luis Rubio; Rusevska, Katerina; Saar, Gunter; Saar, Irja; Salcedo, Isabel; Martinez, Sergio M. Salcedo; Montoya, Carlos A. Salvador; Sanchez-Ramirez, Santiago; Sandoval-Sierra, J. Vladimir; Santamaria, Sergi; Monteiro, Josiane Santana; Schroers, Hans Josef; Schulz, Barbara; Schmidt-Stohn, Geert; Schumacher, Trond; Senn-Irlet, Beatrice; Sevcikova, Hana; Shchepin, Oleg; Shirouzu, Takashi; Shiryaev, Anton; Siepe, Klaus; Sir, Esteban B.; Sohrabi, Mohammad; Soop, Karl; Spirin, Viacheslav; Spribille, Toby; Stadler, Marc; Stalpers, Joost; Stenroos, Soili; Suija, Ave; Sunhede, Stellan; Svantesson, Sten; Svensson, Sigvard; Svetasheva, Tatyana Yu; Swierkosz, Krzysztof; Tamm, Heidi; Taskin, Hatira; Taudiere, Adrien; Tedebrand, Jan-Olof; Lahoz, Raul Tena; Temina, Marina; Thell, Arne; Thines, Marco; Thor, Goren; Thus, Holger; Tibell, Leif; Tibell, Sanja; Timdal, Einar; Tkalcec, Zdenko; Tonsberg, Tor; Trichies, Gerard; Triebel, Dagmar; Tsurykau, Andrei; Tulloss, Rodham E.; Tuovinen, Veera; Sosa, Miguel Ulloa; Urcelay, Carlos; Valade, Francois; Garza, Ricardo Valenzuela; van den Boom, Pieter; Van Vooren, Nicolas; Vasco-Palacios, Aida M.; Vauras, Jukka; Santos, Juan Manuel Velasco; Vellinga, Else; Verbeken, Annemieke; Vetlesen, Per; Vizzini, Alfredo; Voglmayr, Hermann; Volobuev, Sergey; von Brackel, Wolfgang; Voronina, Elena; Walther, Grit; Watling, Roy; Weber, Evi; Wedin, Mats; Weholt, Oyvind; Westberg, Martin; Yurchenko, Eugene; Zehnalek, Petr; Zhang, Huang; Zhurbenko, Mikhail P.; Ekmani, Stefan (2018)
    Nomenclatural type definitions are one of the most important concepts in biological nomenclature. Being physical objects that can be re-studied by other researchers, types permanently link taxonomy (an artificial agreement to classify biological diversity) with nomenclature (an artificial agreement to name biological diversity). Two proposals to amend the International Code of Nomenclature for algae, fungi, and plants (ICN), allowing DNA sequences alone (of any region and extent) to serve as types of taxon names for voucherless fungi (mainly putative taxa from environmental DNA sequences), have been submitted to be voted on at the 11th International Mycological Congress (Puerto Rico, July 2018). We consider various genetic processes affecting the distribution of alleles among taxa and find that alleles may not consistently and uniquely represent the species within which they are contained. Should the proposals be accepted, the meaning of nomenclatural types would change in a fundamental way from physical objects as sources of data to the data themselves. Such changes are conducive to irreproducible science, the potential typification on artefactual data, and massive creation of names with low information content, ultimately causing nomenclatural instability and unnecessary work for future researchers that would stall future explorations of fungal diversity. We conclude that the acceptance of DNA sequences alone as types of names of taxa, under the terms used in the current proposals, is unnecessary and would not solve the problem of naming putative taxa known only from DNA sequences in a scientifically defensible way. As an alternative, we highlight the use of formulas for naming putative taxa (candidate taxa) that do not require any modification of the ICN.
  • Maliniemi, Pilvi; Vincendeau, Michelle; Mayer, Jens; Frank, Oliver; Hahtola, Sonja; Karenko, Leena; Carlsson, Emilia; Mallet, Francois; Seifarth, Wolfgang; Leib-Moesch, Christine; Ranki, Annamari (2013)
  • Helin, Henrik O.; Tuominen, Vilppu J.; Ylinen, Onni; Helin, Heikki; Isola, Jorma (2016)
    Evaluation of human epidermal growth factor receptor 2 (HER2) immunohistochemistry (IHC) is subject to interobserver variation and lack of reproducibility. Digital image analysis (DIA) has been shown to improve the consistency and accuracy of the evaluation and its use is encouraged in current testing guidelines. We studied whether digital image analysis using a free software application (ImmunoMembrane) can assist in interpreting HER2 IHC in equivocal 2+ cases. We also compared digital photomicrographs with whole-slide images (WSI) as material for ImmunoMembrane DIA. We stained 750 surgical resection specimens of invasive breast cancers immunohistochemically for HER2 and analysed staining with ImmunoMembrane. The ImmunoMembrane DIA scores were compared with the originally responsible pathologists' visual scores, a researcher's visual scores and in situ hybridisation (ISH) results. The originally responsible pathologists reported 9.1 % positive 3+ IHC scores, for the researcher this was 8.4 % and for ImmunoMembrane 9.5 %. Equivocal 2+ scores were 34 % for the pathologists, 43.7 % for the researcher and 10.1 % for ImmunoMembrane. Negative 0/1+ scores were 57.6 % for the pathologists, 46.8 % for the researcher and 80.8 % for ImmunoMembrane. There were six false positive cases, which were classified as 3+ by ImmunoMembrane and negative by ISH. Six cases were false negative defined as 0/1+ by IHC and positive by ISH. ImmunoMembrane DIA using digital photomicrographs and WSI showed almost perfect agreement. In conclusion, digital image analysis by ImmunoMembrane can help to resolve a majority of equivocal 2+ cases in HER2 IHC, which reduces the need for ISH testing.
  • Qian, Kui; Pietila, Tuuli; Rönty, Mikko Juhani; Michon, Frederic; Frilander, Mikko J.; Ritari, Jarmo; Tarkkanen, Jussi; Paulin, Lars; Auvinen, Petri; Auvinen, Eeva (2013)
  • Knytl, Martin; Kalous, Lukáš; Rylková, Kateřina; Choleva, Lukáš; Merilä, Juha; Ráb, Petr (2018)
    The crucian carp Carassius carassius (Linnaeus, 1758), is native to many European freshwaters. Despite its wide distribution, the crucian carp is declining in both the number and sizes of populations across much of its range. Here we studied 30 individuals of a putative pure population from Helsinki, Finland. Despite clear external morphological features of C. carassius, an individual was of a higher ploidy level than the others. We therefore applied a set of molecular genetic (S7 nuclear and cytochrome b mitochondrial genes) and cytogenetic tools (sequential fluorescent 4’, 6-diamidino-2-phenylindole [DAPI], Chromomycin A3 [CMA3], C-banding and in situ hybridization [FISH] with both 5S and 28S ribosomal DNA probes) to determine its origin. While all examined characteristics of a diploid representative male (CCAHe2Fi) clearly corresponded to those of C. carassius, a triploid individual (CCAHe1Fi) was more complex. Phylogenetic analysis revealed that the nuclear genome of CCAHe1Fi contained three haploid sets: two C. gibelio and one C. carassius. However the mitochondrial DNA was that of C. gibelio, demonstrating its hybrid origin. The FISH revealed three strong (more intensive) 5S rDNA loci, confirming the triploid status, and an additional 24 weak (less intensive) signals were observed in the chromosome complement of CCAHe1Fi. On the other hand, only two strong and 16 weak 5S rDNA signals were visible on the chromosomes of the CCAHe2Fi male. 28S rDNA FISH revealed four strong signals in both CCAHe1Fi and CCAHe2Fi individuals. CMA3 staining revealed four to six CMA3-positive bands of CCAHe1Fi, while that of diploids contained only two to four. The fact that a polyploid hybrid Carassius female with a strong invasive potential may share morphological characters typical for endangered C. carassius highlights a need to combine genetic investigations of Carassius cryptic diversity with conservation measures of C. carassius in Europe.
  • Sanmark, Enni; Wiksten, Johanna; Välimaa, Hannamari; Blomgren, Karin (2019)
    Aim: The purpose of this prospective study was to determine if there is a difference in number and distribution of salivary bacteria between patients with tonsillar infection and healthy volunteers. Background: The etiology of peritonsillar abscess (PTA) is unclear. Smoking, periodontal disease, and infection of minor salivary glands have been suggested as predisposing factors for PTA. Material and methods: Patients with acute tonsillitis (AT) (n = 54), peritonsillitis (PT) (n = 36), PTA (n = 58), and healthy volunteers (n = 52) were prospectively recruited and evaluated. Saliva bacteria were analyzed with flow cytometry. Patients and their treating physicians completed a questionnaire about patients' current disease, smoking habits, alcohol consumption, and oral health. Results: There were no differences in the total number of saliva bacteria between patients with acute throat infection and healthy volunteers (p = .104) or between AT, PT, and PTA patients (p = .273). Smoking habits, alcohol consumption, oral hygiene, or prior antibiotics had no effect on total amount of salivary bacteria in patients with acute throat infection. Conclusions: The effects of smoking on salivary bacteria do not seem to be the mechanism that promotes development of PTA in smokers.
  • Puttonen, Henri A. J.; Semenova, Svetlana; Sundvik, Maria; Panula, Pertti (2017)
    Monoaminergic neurotransmission is greatly dependent on the function of the vesicular monoamine transporter VMAT2, which is responsible for loading monoamines into secretory vesicles. The role of VMAT2 in histaminergic neurotransmission is poorly understood. We studied the structure and function of the histaminergic system in larval zebrafish following inhibition of VMAT2 function by reserpine. We found that reserpine treatment greatly reduced histamine immunoreactivity in neurons and an almost total disappearance of histamine-containing nerve fibers in the dorsal telencephalon and habenula, the most densely innervated targets of the hypothalamic histamine neurons. The reserpine treated larvae had an impaired histamine-dependent dark-induced flash response seen during the first second after onset of darkness, implying that function of the histaminergic network is VMAT2 dependent. Levels of histamine and other monoamines were decreased in reserpine treated animals. This study provides conclusive evidence of the relevance of VMAT2 in histaminergic neurotransmission, further implying that the storage and release mechanism of neural histamine is comparable to that of other monoamines. Our results also reveal potential new insights about the roles of monoaminergic neurotransmitters in the regulation of locomotion increase during adaptation to darkness.
  • Ján, Labuda; Richard P., Bowater; Miroslav, Fojta; Günter, Gauglitz; Zdeněk, Glatz; Ivan, Hapala; Jan, Havliš; Ferenc, Kilar; Aniko, Kilar; Lenka, Malinovská; Siren, Heli Marja Marita; Petr, Skládal; Federico, Torta; Martin, Valachovič; Michaela, Wimmerová; Zbyněk, Zdráhal; David Brynn, Hibbert (2018)
    Recommendations are given concerning the terminology of methods of bioanalytical chemistry. With respect to dynamic development particularly in the analysis and investigation of biomacromolecules, terms related to bioanalytical samples, enzymatic methods, immunoanalytical methods, methods used in genomics and nucleic acid analysis, proteomics, metabolomics, glycomics, lipidomics, and biomolecules interaction studies are introduced.
  • Brunelli, Matteo; Bria, Emilio; Nottegar, Alessia; Cingarlini, Sara; Simionato, Francesca; Calio, Anna; Eccher, Albino; Parolini, Claudia; Iannucci, Antonio; Gilioli, Eliana; Pedron, Serena; Massari, Francesco; Tortora, Giampaolo; Borze, Florentina Ioana; Knuutila, Sakari; Gobbo, Stefano; Santo, Antonio; Tondulli, Luca; Calabro, Francesco; Martignoni, Guido; Chilosi, Marco (2012)
  • Aranyi, Nikolett Reka; Varga, Ildiko; Poczai, Péter; Cernak, Istvan; Vida, Gyula; Molnar-Lang, Marta; Hoffmann, Borbala (2014)