Browsing by Subject "ISSR POLYMORPHISM"

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  • Qaderi, Ardashir; Omidi, Mansour; Pour-Aboughadareh, Alireza; Poczai, Péter; Shaghaghi, Javad; MehrAfarin, Ali; Nohooji, Majid Ghorbani; Etminan, Alireza (2019)
    In the present investigation, 72 accessions of the Iranian poppy (Papaver bracteatum Lindl.) were analyzed for genetic diversity and population structure using start codon targeted polymorphism (SCoT) and inter simple sequence repeat (ISSR) markers along with four important phytochemical traits to provide baseline knowledge for the Iranian poppy’s breeding and conservation plans. Twelve ISSR and thirteen SCoT primers generated a total of 98 and 186 fragments with a mean of 8.17 and 14.31 fragments per primer, respectively. Polymorphic information content for ISSR and SCoT primers ranged from 0.39 to 0.45 and 0.28 to 0.34, with the resolving power ranging from 21.61 to 3.97 and 13.08 to 28.02, respectively. Neighbour-joining (NJ) based clustering grouped 72 accessions into three main groups based on two markers studied (ISSR and SCoT) and the combined data (ISSR + SCoT), which associated with their eco-geographical regions. Population structure based analysis divided 72 accessions into 3 subpopulations using ISSR markers, when SCoT was used eight subpopulations were observed. However, when the combined data was used only three subpopulations were found, which corresponded to the grouping observed with the NJ method and these results were supported by principal coordinate analyses (PCoA). Phytochemical analysis revealed that plant capsule has higher total amounts of the alkaloids; thebaine, morphine and oripavine than stem tissues. Interestingly, for the geographical parameters, latitude showed a significant and positive correlation with thebaine extracted from both stem and capsules and the regression results confirmed these associations. Taken together, our results indicated that three populations Ploor, Eil-Teymoor and Anjomane due to their high contents of alkaloids like thebaine as well as the Taham population due to its high content of morphine and oripavine have a strong enough potency to be used in the pharmacy industry.