Browsing by Subject "amylase"

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  • Leppäniemi, A.; Tolonen, M.; Tarasconi, A.; Segovia-Lohse, H.; Gamberini, E.; Kirkpatrick, A.W.; Ball, C.G.; Parry, N.; Sartelli, M.; Wolbrink, D.; Van Goor, H.; Baiocchi, G.; Ansaloni, L.; Biffl, W.; Coccolini, F.; Di Saverio, S.; Kluger, Y.; Moore, E.; Catena, F. (2019)
    Although most patients with acute pancreatitis have the mild form of the disease, about 20-30% develops a severe form, often associated with single or multiple organ dysfunction requiring intensive care. Identifying the severe form early is one of the major challenges in managing severe acute pancreatitis. Infection of the pancreatic and peripancreatic necrosis occurs in about 20-40% of patients with severe acute pancreatitis, and is associated with worsening organ dysfunctions. While most patients with sterile necrosis can be managed nonoperatively, patients with infected necrosis usually require an intervention that can be percutaneous, endoscopic, or open surgical. These guidelines present evidence-based international consensus statements on the management of severe acute pancreatitis from collaboration of a panel of experts meeting during the World Congress of Emergency Surgery in June 27-30, 2018 in Bertinoro, Italy. The main topics of these guidelines fall under the following topics: Diagnosis, Antibiotic treatment, Management in the Intensive Care Unit, Surgical and operative management, and Open abdomen. © 2019 The Author(s).
  • Jayachandra, Yaradoddi A.; Sulochana, M. B.; Merja, Kontro H.; Parameshwar, A. B.; Dayanand, Agsar (2020)
    Many halophiles were considered to be extremophiles due to their inborn industrial potentials and tolerance to hostile environmental conditions. The isolated halophilic bacteria described in the present study are not only grown at environmentally adverse conditions, also they can be able to produce bioactive molecules. Among the isolated strains, Oceanobacillus iheyensis strain JAS12 and Salinicoccus roseus strain JS20 are known for the unique biotechnological applications. The isolate Oceanobacillus sp. grows well at 35-55 degrees C (optimum 45 degrees C) and pH 6 to 12 (maximum growth at pH 8), interestingly the strain could hydrolyze casein, starch and gelatin. The G+C content was 40.2 mol % and the major fatty acids are iso-15:0: 30.52%, primary-C15: 0 (29.29 %), iso-14:0 (16.15%) anteiso-C17: 0 (4.03%). Another isolate was Salinicoccus sp. JS20 The DNA G+C content was 50.4 mol % and the major fatty acids are anteiso-C15: 0 (26.23%), iso15:0, (17.62%)Y, 16:0 (11.5%), anteiso-C17: 0 (7.7 %), iso- C16: 0 (10.20 %), iso-17:0: (5.43%) and iso-C14: 0 (3.97 %). These isolates are also producers of many extracellular enzymes such as protease, amylase, inulinases, gelatinase and beta-fructofurinosidase above the optimal conditions. Oceanobacillus sp. JAS12 16S rRNA gene sequence similarity is 99% similar to the reported genera. Salinicoccus sp. JS20 indicated 96% 16S rRNA sequence similarity with near species Salinicoccus genus, thus, they were found to be novel concerning to their genetic makeup and biochemical features.
  • Koivunen, Erja; Talvio, Eija; Valkonen, Eija; Tupasela, Tuomo; Tuunainen, Petra; Valaja, Jarmo (2016)
    The aim was to study the effects of dietary pea inclusion and the addition of Avizyme 1200 -enzyme cocktail on broiler performance, intestinal viscosity and organoleptic quality of meat. The experimental design was a 4 x 2 factorial, the factors being dietary pea inclusion (0, 150, 300 and 450 g kg(-1)) in the diets fed from day 9 to day 38 and the addition of Avizyme 1200 enzyme cocktail including amylase, protease and xylanase during the entire experiment. The growth of birds improved (p 0.05). In conclusion, 450 g kg(-1) peas can be used in the broiler grower diets without negative effects on the bird performance. The use of enzyme cocktail improves bird performance.
  • Geisslitz, Sabrina; Shewry, Peter; Brouns, Fred; America, Antoine H. P.; Caio, Giacomo Pietro Ismaele; Daly, Matthew; D'Amico, Stefano; De Giorgio, Roberto; Gilissen, Luud; Grausgruber, Heinrich; Huang, Xin; Jonkers, Daisy; Keszthelyi, Daniel; Larre, Colette; Masci, Stefania; Mills, Clare; Moller, Marie Sofie; Sorrells, Mark E.; Svensson, Birte; Zevallos, Victor F.; Weegels, Peter Louis (2021)
    Amylase/trypsin-inhibitors (ATIs) comprise about 2-4% of the total wheat grain proteins and may contribute to natural defense against pests and pathogens. However, they are currently among the most widely studied wheat components because of their proposed role in adverse reactions to wheat consumption in humans. ATIs have long been known to contribute to IgE-mediated allergy (notably Bakers' asthma), but interest has increased since 2012 when they were shown to be able to trigger the innate immune system, with attention focused on their role in coeliac disease which affects about 1% of the population and, more recently, in non-coeliac wheat sensitivity which may affect up to 10% of the population. This has led to studies of their structure, inhibitory properties, genetics, control of expression, behavior during processing, effects on human adverse reactions to wheat and, most recently, strategies to modify their expression in the plant using gene editing. We therefore present an integrated account of this range of research, identifying inconsistencies, and gaps in our knowledge and identifying future research needs. Note This paper is the outcome of an invited international ATI expert meeting held in Amsterdam, February 3-5 2020