In this paper, the Spiral of Silence theory (SOS) in the study of mass communications is applied to examine the trends and mechanisms of public opinion in Social Media (SM), using the popular topic of the COVID-19 pandemic. The study includes a secondary analysis of the data on pandemic information consumption obtained through four mass surveys conducted in Armenia. In the period from July 1 to August 30, 2020, we also surveyed Armenian Facebook users by means of Google forms during the highest outbreak of the pandemic in Armenia. In particular, the study demonstrates that although the majority of people are well informed about both public conduct requirements and the sanctions for misconduct during the pandemic, they do not follow the rules but hide their real opinion, preferring to openly agree with the official position while silently breaking the rules (that is, they keep their silence). We have found a correlation between the opinion environment of “friends” and other Facebook users, and a willingness to express their own opinion. Due to the predominance of the self-presentation mode as a communication strategy on Facebook, there is a trend among Armenian users not to risk their reputation, and avoid possible critics by keeping silence, if the discussion goes against their opinion. The findings of the study might be helpful both for the further development of communication theories and its application to the conditions of new pandemic reality, and for a better understanding of communicative behavior mechanisms in SM.

A big question in behavioral ecology is what drives diversity of color signals. One possible explanation is that environmental conditions, such as light environment, may alter visual signaling of prey, which could affect predator decision-making. Here, we tested the context-dependent predator selection on prey coloration. In the first experiment, we tested detectability of artificial visual stimuli to blue tits (Cyanistes caeruleus) by manipulating stimulus luminance and chromatic context of the background. We expected the presence of the chromatic context to facilitate faster target detection. As expected, blue tits found targets on chromatic yellow background faster than on achromatic grey background whereas in the latter, targets were found with smaller contrast differences to the background. In the second experiment, we tested the effect of two light environments on the survival of aposematic, color polymorphic wood tiger moth (Arctia plantaginis). As luminance contrast should be more detectable than chromatic contrast in low light intensities, we expected birds, if they find the moths aversive, to avoid the white morph which is more conspicuous than the yellow morph in low light (and vice versa in bright light). Alternatively, birds may attack first moths that are more detectable. We found birds to attack yellow moths first in low light conditions, whereas white moths were attacked first more frequently in bright light conditions. Our results show that light environments affect predator foraging decisions, which may facilitate context-dependent selection on visual signals and diversity of prey phenotypes in the wild. Light environments are constantly changing and may alter visual appearance of prey, but also bias predators' decision making. Our findings using blue tits in visual search tasks and the wood tiger moth prey under two light environments demonstrate that birds show context-dependent predatory behavior. This suggests that light environments can play a major selective role and influence visual signaling in the wild.

Background: Previous research suggests that parental knowledge of the child's activities and whereabouts prevents adolescents' alcohol use. However, evidence on whether the positive effects of maternal and paternal knowledge are distinctive for boys' and girls' alcohol use is inconclusive. We examined whether perceived parental knowledge at age 13 prevents alcohol use at age 16, whether the effect of maternal and paternal knowledge was the same for both genders, and whether paternal knowledge had as strong an effect as maternal knowledge. Method: Adolescents answered a school survey in 2011 (age 13) and 2014 (age 16) in Finland (N = 5742). Perceived maternal and paternal knowledge was measured separately using a Parents' Monitoring Scale. The data were analysed via moderation regression modelling using Bayesian estimation. Results: Perceived maternal and paternal knowledge at age 13 predicted boys' and girls' lower alcohol use at age 16. For those who had not used alcohol at age 13, parental knowledge protected against an increase of alcohol use at age 16. Both maternal and paternal knowledge had a shielding effect against the increase of boys' and girls' alcohol use, but maternal knowledge had a stronger shielding effect than paternal knowledge. Conclusions: Both maternal and paternal perceived knowledge at age 13 buffers against the adverse development of alcohol use at age 16 for both genders. Underlining the importance of parent-child communication and knowledge about the child's activities should be a part of family health counselling and school health services.

Drying cows off at the end of lactation is a routine management practice in dairy operations. Most dairies in the United States and many other countries dry cows off abruptly (e.g., stop milking cows on a set day), which has been shown to affect cow comfort. Gradually reducing milk production is another approach to dry cows off, routinely used in some countries and herds. The objective of the study was to evaluate the effects of abrupt and gradual milk cessation and milk yield at the time on cow activity after dry-off. Daily lying time, number of lying bouts per day, average lying bout length, and steps taken per day by abruptly and gradually dried-off cows were monitored by data loggers for 2 wk before and after the final milking at the end of lactation. Gradual cows were milked once daily for the last week of lactation, and abrupt cows were milked as usual (3 x /d) until the end of lactation. Gradual cessation of milking significantly reduced milk yield by the day of dry-off. After dry-off, gradual cows tended to have longer lying bouts than abrupt cows, but no other differences in cow activity between the 2 treatments were observed. Regardless of the dry-off method, the average length of a lying bout decreased by 4 min and total daily lying time decreased by 19 min after dry-off for each 5-kg increase in milk yield before dry-off. Lying behavior of primiparous cows was more affected by the level of milk yield at dry-off than that of older cows. A reduction in lying times with increasing milk yield may indicate discomfort due to the accumulating milk in the udder. Using a method that lowers milk production before dry-off and managing primiparous and multiparous cows separately around dry-off are beneficial for cow comfort after dry-off.

Background Emergency care clinicians are expected to use the latest research evidence in practice. However, emergency nurses do not always consistently implement evidence-based practice (EBP). An educational intervention on EBP was implemented to promote emergency nurses' use of EBP, and the effectiveness of it was evaluated. Aims This study aimed to evaluate the effectiveness of an EBP educational intervention on emergency nurses' EBP attitudes, knowledge, self-efficacy, skills, and behavior. The study also examined learners' satisfaction with the EBP educational intervention. Methods A randomized controlled trial with parallel groups with evaluations before the education, immediately after it, and 6 and 12 months after the education was conducted at four emergency departments in two university hospitals. The experimental group (N = 40) received EBP education while the control group (N = 40) completed self-directed EBP education. The primary outcomes were emergency nurses' EBP attitudes, knowledge, self-efficacy, skills, and behavior, while the secondary outcome was satisfaction with the EBP education. Results Thirty-five participants of an experimental and 29 participants of a control group completed the study. There were no statistically significant (p <.05) improvements and differences between groups in EBP attitude, self-efficacy, or behavior immediately after the EBP education. At the 6-month measurement point, the experimental group showed significantly better EBP attitudes, behavior, knowledge, and self-efficacy than the control group. At the 12-month measurement point, the improvements began to decrease. The groups also differed significantly in terms of participant satisfaction with how the teacher encouraged learners to ask clinical questions. Linking Evidence to Action The EBP educational intervention implemented in this study had a positive effect on emergency nurses' EBP attitudes, knowledge, self-efficacy, skills, and behavior. The effects of the education appeared the best 6 months after the education. After this point, the results began to decrease and approached baseline levels. EBP educational interventions designed for emergency nurses should apply various teaching strategies to improve their EBP attitude, knowledge, self-efficacy, skills, behavior, and satisfaction with the education.

Individually ventilated caging (IVC) systems for rodents are increasingly common in laboratory animal facilities. However, the impact of such substantial change in housing conditions on animal physiology and behavior is still debated. Most importantly, there arise the questions regarding reproducibility and comparison of previous or new phenotypes between the IVC and open cages. The present study was set up for detailed and systematic comparison of behavioral phenotypes in male and female mice of three widely used inbred strains (C57BL/6JRccHsd, DBA/2JRccHsd, 129S2/SvHSd) after being kept in two housing environments (IVC and open cages) for 6?weeks (since 4?weeks of age) before behavioral testing. The tests addressed exploratory, anxiety-like and stress-related behavior (light-dark box, open field, forced swim test, stress-induced hyperthermia), social approach and species-specific behavior (nest building, marble burying). In all tests, large and expected strain differences were found. Somewhat surprisingly, the most striking effect of environment was found for basal body temperature and weight loss after one night of single housing in respective cages. In addition, the performance in light-dark box and open field was affected by environment. Several parameters in different tests showed significant interaction between housing and genetic background. In summary, the IVC housing did not invalidate the well-known differences between the mouse strains which have been established by previous studies. However, within the strains the results can be influenced by sex and housing system depending on the behavioral tasks applied. The bottom-line is that the environmental conditions should be described explicitly in all publications.

The importance of equine welfare has become more important in the last years. There is a need for welfare parameters, which help to define and measure the welfare of domestic horses. The importance of sleep on health and wellbeing is well-known in humans but has not yet been extensively studied in horses. It is known that horses sleep either non-REM-sleep or REM-sleep. Also, horses are able to partially sleep in a standing position. For REM-sleep they need to have muscle atony and lie down. Horses are easily disturbed while sleeping and many factors affect how much and how long horses spend sleeping. Horses are also able to postpone their REM-sleep for extensive periods of time, which directly effects their health and welfare. The aim of our study was to measure and analyze how the softness of the bedding in the lying areas affect the sleeping and resting behavior of horses. This thesis was part of the UNIHEPO initiative, which consisted of multiple studies around equine sleep. For our study we conducted a cross over study with sixteen (16) clinically healthy horses in the equine school Ylä-Savon ammattiopisto during fall 2022. The study included three treatments: the normal amount of bedding as the baseline, then thin (5 cm) bedding and thick (15 cm) of bedding. We recorded three periods: the baseline, and then two consecutive periods with half of the stalls having thick bedding and the other half thin. The duration of each treatment period was 21 days, respectively. We switched the treatments after the first period so that that each horse had both treatments. We recorded and analyzed the first two (2) and last two (2) nights of each period. The results were reported as seconds calculated from the median of the daily mean values. Only the data from the two treatments was analyzed for this thesis. The horses exhibited more resting behaviors and supported their necks longer in a sleeping position, when the bedding was thicker (p=0,002). There was no statistically significant difference between the treatments when lateral recumbency bout amounts or lying durations were analyzed, but the lying duration was longer. With thicker bedding the horses had a higher number of sternal recumbency bouts (p=0,013) and the bout duration was longer (p=0,001). Also, the total duration spent in sternal recumbency was higher on thicker bedding (p=0,002). Surprisingly we noticed rolling behavior after lying bouts almost solely on thicker bedding (p=0,004). There were also some tendencies for correlation between the height of the horses, lying bouts and bedding thickness. Our research provided us with valuable information on the factors affecting the sleeping and resting behavior of horses. At the same time the need for further research was highlighted. Still, our results reinforce the scientific knowledge, which is crucial in developing and promoting equine welfare.

BackgroundThe focus in occupational health check-ups is in work and health, but they offer also a possibility to assess health behavior and give guidance e.g. on weight control. We wanted to study whether having occupational health checks-up, receiving physicians' advice to change health behavior or participation in health promotion programs had an effect on obesity in a five-year follow-up from 1998 to 2003 in asthmatic and non-asthmatic workers.MethodsAltogether 23,220 individuals aged 20-54years were picked up from a randomized Finnish population sample. Univariate and multivariate logistic regression analysis was used to calculate the risk for obesity in 2003. The variables used in the modelling were gender, age, smoking, asthma, depression, and physical workload.ResultsBoth asthmatic and non-asthmatic workers gained weight during the follow-up. Of the asthmatics 48 and 47% of the non-asthmatics had occupational health-check-up in the last 5years. Of the asthmatics 18 and 14% of the non-asthmatics had received physician's advice to change their health behavior (p30) in 2003 were gender (men OR 1.19), older age (OR 1.25), smoking (OR 1.07) or depression (OR 1.44).ConclusionsResults show that having occupational health checks-up or receiving physicians' advice to change health behavior or participation in health promotion programs did not stop gain of weight during a five-year follow-up. Asthmatic workers did not differ from non-asthmatics. Male gender, older age, smoking, and depression were associated with obesity but not the physical workload.

Cryptochrome 2 (Cry2) is a core clock gene important for circadian regulation. It has also been associated with anxiety and depressive-like behaviors in mice, but the previous findings have been conflicting in terms of the direction of the effect. To begin to elucidate the molecular mechanisms of this association, we carried out behavioral testing, PET imaging, and gene expression analysis of Cry2(-/-) and Cry2(+/+) mice. Compared to Cry2(+/+) mice, we found that Cry2(-/-) mice spent less time immobile in the forced swim test, suggesting reduced despair-like behavior. Moreover, Cry2(-/-) mice had lower saccharin preference, indicative of increased anhedonia. In contrast, we observed no group differences in anxiety-like behavior. The behavioral changes were accompanied by lower metabolic activity of the ventro-medial hypothalamus, suprachiasmatic nuclei, ventral tegmental area, anterior and medial striatum, substantia nigra, and habenula after cold stress as measured by PET imaging with a glucose analog. Although the expression of many depression-associated and metabolic genes was upregulated or downregulated by cold stress, we observed no differences between Cry2(-/-) and Cry2(+/+) mice. These findings are consistent with other studies showing that Cry2 is required for normal emotional behavior. Our findings confirm previous roles of Cry2 in behavior and extend them by showing that the effects on behavior may be mediated by changes in brain metabolism.

Animal models of neurodegenerative and neuropsychiatric disorders require extensive behavioral phenotyping. Currently, this presents several caveats and the most important are: (i) rodents are nocturnal animals, but mostly tested during the light period; (ii) the conventional behavioral experiments take into consideration only a snapshot of a rich behavioral repertoire; and (iii) environmental factors, as well as experimenter influence, are often underestimated. Consequently, serious concerns have been expressed regarding the reproducibility of research findings on the one hand, and appropriate welfare of the animals (based on the principle of 3Rs—reduce, refine and replace) on the other hand. To address these problems and improve behavioral phenotyping in general, several solutions have been proposed and developed. Undisturbed, 24/7 home-cage monitoring (HCM) is gaining increased attention and popularity as demonstrating the potential to substitute or complement the conventional phenotyping methods by providing valuable data for identifying the behavioral patterns that may have been missed otherwise. In this review, we will briefly describe the different technologies used for HCM systems. Thereafter, based on our experience, we will focus on two systems, IntelliCage (NewBehavior AG and TSE-systems) and Digital Ventilated Cage (DVC®, Tecniplast)—how they have been developed and applied during recent years. Additionally, we will touch upon the importance of the environmental/experimenter artifacts and propose alternative suggestions for performing phenotyping experiments based on the published evidence. We will discuss how the integration of telemetry systems for deriving certain physiological parameters can help to complement the description of the animal model to offer better translation to human studies. Ultimately, we will discuss how such HCM data can be statistically interpreted and analyzed.