Browsing by Subject "consortia"

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  • Kaya, Meryem Ecem (Helsingin yliopisto, 2019)
    Synthetic biology is an emerging interdisciplinary field of biology that aims to system-atically design artificial biological systems. As synthetic biologists seek increasingly complex control over cellular processes to achieve robust and predictable systems. A new frontier in synthetic biology is engineering synthetic microbial consortia. This ap-proach employs the concept of division of labor, instead of introducing large genetic cir-cuitry to homogenous cell populations. In this approach, different cell types are assigned to execute a portion of the overall circuit. Each cell type communicates with their co-worker subpopulations to complete the circuit. The main advantage of this strategy is the reduced metabolic burden on each cell type. Thus, leading to more reliable and stable overall performance. In this work, to simplify cellular communication between the mem-bers of the consortium, we used the simple architecture of quorum sensing machinery. We constructed a toolbox that contains promoter, receptor and quorum sensing signal synthase genes along with fluorescent reporters. Using this toolbox, we constructed dif-ferent cell types that can be used in synthetic consortia forming various communication topologies. We characterized the constructed cell types individually and in co-cultures.
  • Ilva, Jyrki (2018)
    Most of the 100+ Finnish scholarly journals are published by small learned societies. Since 2015, the National Library of Finland and the Federation of Finnish Learned Societies have been working on a joint project which aims to provide the journals with the support they need for making a transition to open access. The project has launched an OJS-based shared publication platform (Journal.fi), which is already used by 50 journals. It has also been developing a new funding model for the journals. Since the subscription and licensing costs paid by the research libraries for these journals have been very small, it is not possible to simply use these funds to pay for open access. Instead, the project has been working on a consortium-based model, under which the Finnish research organizations and funders would commit themselves to providing long-term funding to the journals. In return, the journals would pledge to follow strict standards in openness, licensing, peer review and infrastructure.
  • Kelemen, Linda E.; Earp, Madalene; Fridley, Brooke L.; Chenevix-Trench, Georgia; Fasching, Peter A.; Beckmann, Matthias W.; Ekici, Arif B.; Hein, Alexander; Lambrechts, Diether; Lambrechts, Sandrina; Van Nieuwenhuysen, Els; Vergote, Ignace; Rossing, Mary Anne; Doherty, Jennifer A.; Chang-Claude, Jenny; Behrens, Sabine; Moysich, Kirsten B.; Cannioto, Rikki; Lele, Shashikant; Odunsi, Kunle; Goodman, Marc T.; Shvetsov, Yurii B.; Thompson, Pamela J.; Wilkens, Lynne R.; Doerk, Thilo; Antonenkova, Natalia; Bogdanova, Natalia; Hillemanns, Peter; Runnebaum, Ingo B.; du Bois, Andreas; Harter, Philipp; Heitz, Florian; Schwaab, Ira; Butzow, Ralf; Pelttari, Liisa M.; Nevanlinna, Heli; Modugno, Francesmary; Edwards, Robert P.; Kelley, Joseph L.; Ness, Roberta B.; Karlan, Beth Y.; Lester, Jenny; Orsulic, Sandra; Walsh, Christine; Kjaer, Susanne K.; Jensen, Allan; Cunningham, Julie M.; Vierkant, Robert A.; Giles, Graham G.; Bruinsma, Fiona (2018)
    Thymidylate synthase (TYMS) is a crucial enzyme for DNA synthesis. TYMS expression is regulated by its antisense mRNA, ENOSF1. Disrupted regulation may promote uncontrolled DNA synthesis and tumor growth. We sought to replicate our previously reported association between rs495139 in the TYMS-ENOSF1 3' gene region and increased risk of mucinous ovarian carcinoma (MOC) in an independent sample. Genotypes from 24,351 controls to 15,000 women with invasive OC, including 665 MOC, were available. We estimated per-allele odds ratios (OR) and 95% confidence intervals (CI) using unconditional logistic regression, and meta-analysis when combining these data with our previous report. The association between rs495139 and MOC was not significant in the independent sample (OR = 1.09; 95% CI = 0.97-1.22; p = 0.15; N = 665 cases). Meta-analysis suggested a weak association (OR = 1.13; 95% CI = 1.03-1.24; p = 0.01; N = 1019 cases). No significant association with risk of other OC histologic types was observed (p = 0.05 for tumor heterogeneity). In expression quantitative trait locus (eQTL) analysis, the rs495139 allele was positively associated with ENOSF1 mRNA expression in normal tissues of the gastrointestinal system, particularly esophageal mucosa (r = 0.51, p = 1.7 x 10(-28)), and nonsignificantly in five MOC tumors. The association results, along with inconclusive tumor eQTL findings, suggest that a true effect of rs495139 might be small.