Browsing by Subject "oxygen depletion"

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  • Barmaki, Samineh; Obermaier, Daniela; Kankuri, Esko; Vuola, Jyrki; Franssila, Sami; jokinen, Ville (2020)
    A hypoxic (low oxygen level) microenvironment and nitric oxide paracrine signaling play important roles in the control of both biological and pathological cell responses. In this study, we present a microfluidic chip architecture for nitric oxide delivery under a hypoxic microenvironment in human embryonic kidney cells (HEK-293). The chip utilizes two separate, but interdigitated microfluidic channels. The hypoxic microenvironment was created by sodium sulfite as the oxygen scavenger in one of the channels. The nitric oxide microenvironment was created by sodium nitroprusside as the light-activated nitric oxide donor in the other channel. The solutions are separated from the cell culture by a 30 µm thick gas-permeable, but liquid-impermeable polydimethylsiloxane membrane. We show that the architecture is preliminarily feasible to define the gaseous microenvironment of a cell culture in the 100 µm and 1 mm length scales.
  • Pilla, Rachel M.; Mette, Elizabeth M.; Williamson, Craig E.; Adamovich, Boris V.; Adrian, Rita; Anneville, Orlane; Balseiro, Esteban; Ban, Syuhei; Chandra, Sudeep; Colom-Montero, William; Devlin, Shawn P.; Dix, Margaret A.; Dokulil, Martin T.; Feldsine, Natalie A.; Feuchtmayr, Heidrun; Fogarty, Natalie K.; Gaiser, Evelyn E.; Girdner, Scott F.; González, María J.; Hambright, K. David; Hamilton, David P.; Havens, Karl; Hessen, Dag O.; Hetzenauer, Harald; Higgins, Scott N.; Huttula, Timo H.; Huuskonen, Hannu; Isles, Peter D. F.; Joehnk, Klaus D.; Keller, Wendel Bill; Klug, Jen; Knoll, Lesley B.; Korhonen, Johanna; Korovchinsky, Nikolai M.; Köster, Oliver; Kraemer, Benjamin M.; Leavitt, Peter R.; Leoni, Barbara; Lepori, Fabio; Lepskaya, Ekaterina V.; Lottig, Noah R.; Luger, Martin S.; Maberly, Stephen C.; MacIntyre, Sally; McBride, Chris; McIntyre, Peter; Melles, Stephanie J.; Modenutti, Beatriz; Müller-Navarra, Dörthe C.; Pacholski, Laura; Paterson, Andrew M.; Pierson, Don C.; Pislegina, Helen V.; Plisnier, Pierre-Denis; Richardson, David C.; Rimmer, Alon; Rogora, Michela; Rogozin, Denis Y.; Rusak, James A.; Rusanovskaya, Olga O.; Sadro, Steve; Salmaso, Nico; Saros, Jasmine E.; Sarvala, Jouko; Saulnier-Talbot, Émilie; Schindler, Daniel E.; Shimaraeva, Svetlana V.; Silow, Eugene A.; Sitoki, Lewis M.; Sommaruga, Ruben; Straile, Dietmar; Strock, Kristin E.; Swain, Hilary; Tallant, Jason M.; Thiery, Wim; Timofeyev, Maxim A.; Tolomeev, Alexander P.; Tominaga, Koji; Vanni, Michael J.; Verburg, Piet; Vinebrooke, Rolf D.; Wanzenböck, Josef; Weathers, Kathleen; Weyhenmeyer, Gesa A.; Zadereev, Egor S.; Zhukova, Tatyana V. (Nature, 2021)
    Scientific Data 8 (2021), 200
    Climate change and other anthropogenic stressors have led to long-term changes in the thermal structure, including surface temperatures, deepwater temperatures, and vertical thermal gradients, in many lakes around the world. Though many studies highlight warming of surface water temperatures in lakes worldwide, less is known about long-term trends in full vertical thermal structure and deepwater temperatures, which have been changing less consistently in both direction and magnitude. Here, we present a globally-expansive data set of summertime in-situ vertical temperature profiles from 153 lakes, with one time series beginning as early as 1894. We also compiled lake geographic, morphometric, and water quality variables that can influence vertical thermal structure through a variety of potential mechanisms in these lakes. These long-term time series of vertical temperature profiles and corresponding lake characteristics serve as valuable data to help understand changes and drivers of lake thermal structure in a time of rapid global and ecological change.