Ophiobolin A from Bipolaris oryzae Perturbs Motility and Membrane Integrities of Porcine Sperm and Induces Cell Death on Mammalian Somatic Cell Lines

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Bencsik , O , Papp , T , Berta , M , Zana , A , Forgo , P , Dombi , G , Andersson , M , Salkinoja-Salonen , M S , Vagvölgyi , C & Szekers , A 2014 , ' Ophiobolin A from Bipolaris oryzae Perturbs Motility and Membrane Integrities of Porcine Sperm and Induces Cell Death on Mammalian Somatic Cell Lines ' , Toxins , vol. 6 , no. 9 , pp. 2857-2871 . https://doi.org/10.3390/toxins6092857 , https://doi.org/10.3390/toxins6092857

Title: Ophiobolin A from Bipolaris oryzae Perturbs Motility and Membrane Integrities of Porcine Sperm and Induces Cell Death on Mammalian Somatic Cell Lines
Author: Bencsik, Otto; Papp, Tamas; Berta, Mate; Zana, Annamaria; Forgo, Peter; Dombi, György; Andersson, Maria; Salkinoja-Salonen, Mirja Sinikka; Vagvölgyi, Csaba; Szekers, András
Contributor: University of Helsinki, Department of Food and Nutrition
University of Helsinki, Department of Food and Nutrition
Date: 2014-09-23
Language: eng
Number of pages: 15
Belongs to series: Toxins
ISSN: 2072-6651
URI: http://hdl.handle.net/10138/155642
Abstract: Abstract: Bipolaris oryzae is a phytopathogenic fungus causing a brown spot disease in rice, and produces substance that strongly perturbs motility and membrane integrities of boar spermatozoa. The substance was isolated from the liquid culture of the fungal strain using extraction and a multi-step semi-preparative HPLC procedures. Based on the results of mass spectrometric and 2D NMR techniques, the bioactive molecule was identified as ophiobolin A, a previously described sesterterpene-type compound. The purified ophiobolin A exhibited strong motility inhibition and viability reduction on boar spermatozoa. Furthermore, it damaged the sperm mitochondria significantly at sublethal concentration by the dissipation of transmembrane potential in the mitochondrial inner membrane, while the plasma membrane permeability barrier remained intact. The study demonstrated that the cytotoxicity of ophiobolin A toward somatic cell lines is higher by 1–2 orders of magnitude compared to other mitochondriotoxic mycotoxins, and towards sperm cells unique by replacing the progressive motility by shivering tail beating at low exposure concentration.
Subject: 1183 Plant biology, microbiology, virology
mitochondrial toxicity
SPERM CAPACITATION
sperm motility
alamethicin
enniatin B
acrebol
trilongin
mycotoxin
toxicity assay
sperm toxicity
boar spermatozoa
fungal toxin
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