Genetic import and phenotype specific alleles associated with hyper-invasion in Campylobacter jejuni

Show simple item record

dc.contributor.author Baig, Abiyad
dc.contributor.author McNally, Alan
dc.contributor.author Dunn, Steven
dc.contributor.author Paszkiewicz, Konrad H
dc.contributor.author Corander, Jukka
dc.contributor.author Manning, Georgina
dc.date.accessioned 2015-10-24T04:35:12Z
dc.date.available 2015-10-24T04:35:12Z
dc.date.issued 2015-10-24
dc.identifier.citation BMC Genomics. 2015 Oct 24;16(1):852
dc.identifier.uri http://hdl.handle.net/10138/157555
dc.description.abstract Abstract Background Campylobacter jejuni is a major zoonotic pathogen, causing gastroenteritis in humans. Invasion is an important pathogenesis trait by which C. jejuni causes disease. Here we report the genomic analysis of 134 strains to identify traits unique to hyperinvasive isolates. Methods A total of 134 C. jejuni genomes were used to create a phylogenetic tree to position the hyperinvasive strains. Comparative genomics lead to the identification of mosaic capsule regions. A pan genome approach led to the discovery of unique loci, or loci with unique alleles, to the hyperinvasive strains. Results Phylogenetic analysis showed that the hyper-invasive phenotype is a generalist trait. Despite the fact that hyperinvasive strains are only distantly related based on the whole genome phylogeny, they all possess genes within the capsule region with high identity to capsule genes from C. jejuni subsp. doylei and C. lari. In addition there were genes unique to the hyper-invasive strains with identity to non-C. jejuni genes, as well as allelic variants of mainly pathogenesis related genes already known in the other C. jejuni. In particular, the sequence of flagella genes, flgD-E and flgL were highly conserved amongst the hyper-invasive strains and divergent from sequences in other C. jejuni. A novel cytolethal distending toxin (cdt) operon was also identified as present in all hyper-invasive strains in addition to the classic cdt operon present in other C. jejuni. Conclusions Overall, the hyper-invasive phenotype is strongly linked to the presence of orthologous genes from other Campylobacter species in their genomes, notably within the capsule region, in addition to the observed association with unique allelic variants in flagellar genes and the secondary cdt operon which is unlikely under random sharing of accessory alleles in separate lineages.
dc.publisher BioMed Central
dc.title Genetic import and phenotype specific alleles associated with hyper-invasion in Campylobacter jejuni
dc.date.updated 2015-10-24T04:35:12Z
dc.language.rfc3066 en
dc.rights.holder Baig et al.
dc.type.uri http://purl.org/eprint/entityType/ScholarlyWork
dc.type.uri http://purl.org/eprint/entityType/Expression
dc.type.uri http://purl.org/eprint/type/JournalArticle

Files in this item

Total number of downloads: Loading...

Files Size Format View
12864_2015_Article_2087.pdf 1.653Mb PDF View/Open

This item appears in the following Collection(s)

Show simple item record