The effect of E3 ligases on Arabidopsis thaliana yield parameters

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http://urn.fi/URN:NBN:fi:hulib-201511163814
Title: The effect of E3 ligases on Arabidopsis thaliana yield parameters
Author: Lindholm, Katariina Marianne
Contributor: University of Helsinki, Faculty of Agriculture and Forestry, Department of Agricultural Sciences
Publisher: Helsingin yliopisto
Date: 2015
Language: eng
URI: http://urn.fi/URN:NBN:fi:hulib-201511163814
http://hdl.handle.net/10138/158136
Thesis level: master's thesis
Discipline: Växtproduktionsvetenskap
Plant Production Science
Kasvintuotantotieteet
Abstract: Arabidopsis (Arabidopsis thaliana J. Thal) is a common model species in plant research. Although its genome is completely sequenced, the complete effects of some genes remain undetermined. The aim of this study was to investigate the role of several E3 Ubiquitin (Ub) ligases in Arabidopsis thaliana development through insertional mutant lines from the SALK collection. Sixteen mutant lines in a wild-type Columbia-0 (Col-0) background were subjected to genotyping, and the subsequent confirmed homozygous lines were analyzed for developmental phenotype. Greenhouse experiments were conducted on the wild type Columbia (Col) and sixteen mutant lines. Yield parameters such as days to bolting and silique surface area were studied to identify differences among the mutant lines relative to wild-type. After phenotyping, statistical analysis was performed to determine if there were any significant differences among the lines. The mean of both number of days to bolting and average silique surface area, among line 8, line 9, and line 13b plants, were shown by one way ANOVA to be significantly different than wild-type. These results suggest that those E3 ubiquitin ligases might be involved in the regulation of plant growth and development.
Subject: Arabidopsis thaliana
ubiquitin
E3
ligase
bolting
flowering time


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