Online measurements of real-time cytotoxic responses induced by multi-component matrices, such as natural products, through electric-cell substrate impedance sensing (ECIS).

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Fallarero , A , Batista-González , A , Hiltunen , A K , Liimatainen , J , Karonen , M & Vuorela , P M 2015 , ' Online measurements of real-time cytotoxic responses induced by multi-component matrices, such as natural products, through electric-cell substrate impedance sensing (ECIS). ' , International Journal of Molecular Sciences , vol. 16 , no. 11 , pp. 27044-27057 . https://doi.org/10.3390/ijms161126014

Title: Online measurements of real-time cytotoxic responses induced by multi-component matrices, such as natural products, through electric-cell substrate impedance sensing (ECIS).
Author: Fallarero , Adyary; Batista-González, Ana; Hiltunen, Anna K.; Liimatainen, Jaana; Karonen, Maarit; Vuorela, Pia M.
Contributor organization: Faculty of Pharmacy
Division of Pharmaceutical Biosciences
Pharmaceutical Design and Discovery group
Explorations of Anti Infectives
Drug Research Program
Date: 2015
Language: eng
Number of pages: 14
Belongs to series: International Journal of Molecular Sciences
ISSN: 1422-0067
DOI: https://doi.org/10.3390/ijms161126014
URI: http://hdl.handle.net/10138/159333
Abstract: Natural products are complex matrices of compounds that are prone to interfere with the label-dependent methods that are typically used for cytotoxicity screenings. Here, we developed a label-free Electric Cell-substrate Impedance Sensing (ECIS)-based cytotoxicity assay that can be applied in the assessment of the cytotoxicity of natural extracts. The conditions to measure the impedance using ECIS were first optimized in mice immortalized hypothalamic neurons GT1-7 cells. The performance of four natural extracts when tested using three conventional cytotoxicity assays in GT1-7 cells, was studied. Betula pendula (silver birch tree) was found to interfere with all of the cytotoxicity assays in which labels were applied. The silver birch extract was also proven to be cytotoxic and, thus, served as a proof-of-concept for the use of ECIS. The extract was fractionated and the ECIS method permitted the distinction of specific kinetic patterns of cytotoxicity on the fractions as well as the extract's pure constituents. This study offers evidence that ECIS is an excellent tool for real-time monitoring of the cytotoxicity of complex extracts that are difficult to work with using conventional (label-based) assays. Altogether, it offers a very suitable cytotoxicity-screening assay making the work with natural products less challenging within the drug discovery workflow.
Subject: 1182 Biochemistry, cell and molecular biology
317 Pharmacy
ECIS
cytotoxicity
impedance
label-free
natural extracts
screening
IN-VITRO-CYTOTOXICITY
OXIDATIVE STRESS
ENDOTHELIAL CELLS
SCREENING ASSAY
DRUG DISCOVERY
LEAF EXTRACT
INNER BARK
MTT
APOPTOSIS
PROPOLIS
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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