Bioactive cembrane derivatives from the Indian Ocean soft coral, Sinularia kavarattiensis

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Lillsunde , K-E , Festa , C , Adel , H , De Marino , S , Lombardi , V , Tilvi , S , Nawrot , D , Zampella , A , D'Souza , L , D'Auria , M V & Tammela , P 2014 , ' Bioactive cembrane derivatives from the Indian Ocean soft coral, Sinularia kavarattiensis ' , Marine Drugs , vol. 12 , no. 7 , pp. 4045-4068 . https://doi.org/10.3390/md12074045

Title: Bioactive cembrane derivatives from the Indian Ocean soft coral, Sinularia kavarattiensis
Author: Lillsunde, Katja-Emilia; Festa, Carmen; Adel, Harshada; De Marino, Simona; Lombardi, Valter; Tilvi, Supriya; Nawrot, Dorota; Zampella, Angela; D'Souza, Lisette; D'Auria, Maria Valeria; Tammela, Päivi
Contributor: University of Helsinki, Faculty of Pharmacy
University of Helsinki, Faculty of Pharmacy
University of Helsinki, Faculty of Pharmacy
Date: 2014-07-03
Language: eng
Number of pages: 24
Belongs to series: Marine Drugs
ISSN: 1660-3397
URI: http://hdl.handle.net/10138/159430
Abstract: Marine organisms and their metabolites represent a unique source of potential pharmaceutical substances. In this study, we examined marine-derived substances for their bioactive properties in a cell-based Chikungunya virus (CHIKV) replicon model and for in vitro anti-inflammatory activity. In the screening of a marine sample library, crude extracts from the Indian soft coral, Sinularia kavarattiensis, showed promising activity against the CHIKV replicon. Bioassay-guided chemical fractionation of S. kavarattiensis resulted in the isolation of six known norcembranoids (1–6) and one new compound, named kavaranolide (7). The structures were elucidated on the basis of NMR and MS spectroscopic data. Compounds 1–3 and 5–7 were evaluated for their replicon-inhibiting potential in the CHIKV model by using a luminescence-based detection technique and live cell imaging. Compounds 1 and 2 showed moderate inhibition of the CHIKV replicon, but imaging studies also revealed cytotoxic properties. Moreover, the effects of the isolated compounds on primary microglial cells, an experimental model for neuroinflammation, were evaluated. Compound 2 was shown to modulate the immune response in microglial cells and to possess potential anti-inflammatory properties by dose-dependently reducing the release of pro- and anti-inflammatory cytokines.
Subject: 317 Pharmacy
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