Analysis of indole alkaloids from Rhazya stricta hairy roots by ultra-performance liquid chromatography-mass spectrometry

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Akhgari , A , Laakso , I , Seppänen-Laakso , T , Yrjönen , T , Vuorela , H , Oksman-Caldentey , K-M & Rischer , H 2015 , ' Analysis of indole alkaloids from Rhazya stricta hairy roots by ultra-performance liquid chromatography-mass spectrometry ' , Molecules , vol. 20 , no. 12 , pp. 22621-22634 . https://doi.org/10.3390/molecules201219873

Title: Analysis of indole alkaloids from Rhazya stricta hairy roots by ultra-performance liquid chromatography-mass spectrometry
Author: Akhgari, Amir; Laakso, Into; Seppänen-Laakso, Tuulikki; Yrjönen, Teijo; Vuorela, Heikki; Oksman-Caldentey, Kirsi-Marja; Rischer, Heiko
Other contributor: University of Helsinki, Faculty of Pharmacy
University of Helsinki, Faculty of Pharmacy
University of Helsinki, Faculty of Pharmacy
University of Helsinki, Faculty of Pharmacy



Date: 2015-12-18
Language: eng
Number of pages: 14
Belongs to series: Molecules
ISSN: 1420-3049
DOI: https://doi.org/10.3390/molecules201219873
URI: http://hdl.handle.net/10138/159525
Abstract: Rhazya stricta Decne. (Apocynaceae) contains a large number of terpenoid indole alkaloids (TIAs). This study focused on the composition of alkaloids obtained from transformed hairy root cultures of R. stricta employing ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). In the UPLC-MS analyses, a total of 20 TIAs were identified from crude extracts. Eburenine and vincanine were the main alkaloids followed by polar glucoalkaloids, strictosidine lactam and strictosidine. Secodine-type alkaloids, tetrahydrosecodinol, tetrahydro- and dihydrosecodine were detected too. The occurrence of tetrahydrosecodinol was confirmed for the first time for R. stricta. Furthermore, two isomers of yohimbine, serpentine and vallesiachotamine were identified. The study shows that a characteristic pattern of biosynthetically related TIAs can be monitored in Rhazya hairy root crude extract by this chromatographic method.
Subject: 317 Pharmacy
116 Chemical sciences
Rhazya stricta
terpenoid indole alkaloids
ultra-performance liquid chromatography-mass spectrometry
CATHARANTHUS-ROSEUS
CELL-CULTURES
ALSTONIA-SCHOLARIS
SECODINE-TYPE
YOHIMBE BARK
BIOSYNTHESIS
QUANTIFICATION
IDENTIFICATION
EXTRACT
LINE
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