Akhgari , A , Laakso , I , Seppänen-Laakso , T , Yrjönen , T , Vuorela , H , Oksman-Caldentey , K-M & Rischer , H 2015 , ' Analysis of indole alkaloids from Rhazya stricta hairy roots by ultra-performance liquid chromatography-mass spectrometry ' , Molecules , vol. 20 , no. 12 , pp. 22621-22634 . https://doi.org/10.3390/molecules201219873
Title: | Analysis of indole alkaloids from Rhazya stricta hairy roots by ultra-performance liquid chromatography-mass spectrometry |
Author: | Akhgari , Amir; Laakso, Into; Seppänen-Laakso, Tuulikki; Yrjönen, Teijo; Vuorela, Heikki; Oksman-Caldentey, Kirsi-Marja; Rischer, Heiko |
Contributor organization: | Faculty of Pharmacy Division of Pharmaceutical Biosciences Pharmaceutical Design and Discovery group Drug Research Program |
Date: | 2015-12-18 |
Language: | eng |
Number of pages: | 14 |
Belongs to series: | Molecules |
ISSN: | 1420-3049 |
DOI: | https://doi.org/10.3390/molecules201219873 |
URI: | http://hdl.handle.net/10138/159525 |
Abstract: | Rhazya stricta Decne. (Apocynaceae) contains a large number of terpenoid indole alkaloids (TIAs). This study focused on the composition of alkaloids obtained from transformed hairy root cultures of R. stricta employing ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). In the UPLC-MS analyses, a total of 20 TIAs were identified from crude extracts. Eburenine and vincanine were the main alkaloids followed by polar glucoalkaloids, strictosidine lactam and strictosidine. Secodine-type alkaloids, tetrahydrosecodinol, tetrahydro- and dihydrosecodine were detected too. The occurrence of tetrahydrosecodinol was confirmed for the first time for R. stricta. Furthermore, two isomers of yohimbine, serpentine and vallesiachotamine were identified. The study shows that a characteristic pattern of biosynthetically related TIAs can be monitored in Rhazya hairy root crude extract by this chromatographic method. |
Subject: |
317 Pharmacy
116 Chemical sciences Rhazya stricta terpenoid indole alkaloids ultra-performance liquid chromatography-mass spectrometry CATHARANTHUS-ROSEUS CELL-CULTURES ALSTONIA-SCHOLARIS SECODINE-TYPE YOHIMBE BARK BIOSYNTHESIS QUANTIFICATION IDENTIFICATION EXTRACT LINE |
Peer reviewed: | Yes |
Rights: | cc_by |
Usage restriction: | openAccess |
Self-archived version: | publishedVersion |
Total number of downloads: Loading...
Files | Size | Format | View |
---|---|---|---|
molecules_20_19873.pdf | 1.346Mb |
View/ |