Analysis of indole alkaloids from Rhazya stricta hairy roots by ultra-performance liquid chromatography-mass spectrometry

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dc.contributor.author Akhgari , Amir
dc.contributor.author Laakso, Into
dc.contributor.author Seppänen-Laakso, Tuulikki
dc.contributor.author Yrjönen, Teijo
dc.contributor.author Vuorela, Heikki
dc.contributor.author Oksman-Caldentey, Kirsi-Marja
dc.contributor.author Rischer, Heiko
dc.date.accessioned 2016-01-19T10:58:01Z
dc.date.available 2016-01-19T10:58:01Z
dc.date.issued 2015-12-18
dc.identifier.citation Akhgari , A , Laakso , I , Seppänen-Laakso , T , Yrjönen , T , Vuorela , H , Oksman-Caldentey , K-M & Rischer , H 2015 , ' Analysis of indole alkaloids from Rhazya stricta hairy roots by ultra-performance liquid chromatography-mass spectrometry ' , Molecules , vol. 20 , no. 12 , pp. 22621-22634 . https://doi.org/10.3390/molecules201219873
dc.identifier.other PURE: 58090339
dc.identifier.other PURE UUID: 84aeeaa4-de32-481d-a538-271896c49c86
dc.identifier.other WOS: 000367533400119
dc.identifier.other Scopus: 84954324976
dc.identifier.uri http://hdl.handle.net/10138/159525
dc.description.abstract Rhazya stricta Decne. (Apocynaceae) contains a large number of terpenoid indole alkaloids (TIAs). This study focused on the composition of alkaloids obtained from transformed hairy root cultures of R. stricta employing ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). In the UPLC-MS analyses, a total of 20 TIAs were identified from crude extracts. Eburenine and vincanine were the main alkaloids followed by polar glucoalkaloids, strictosidine lactam and strictosidine. Secodine-type alkaloids, tetrahydrosecodinol, tetrahydro- and dihydrosecodine were detected too. The occurrence of tetrahydrosecodinol was confirmed for the first time for R. stricta. Furthermore, two isomers of yohimbine, serpentine and vallesiachotamine were identified. The study shows that a characteristic pattern of biosynthetically related TIAs can be monitored in Rhazya hairy root crude extract by this chromatographic method. en
dc.format.extent 14
dc.language.iso eng
dc.relation.ispartof Molecules
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject 317 Pharmacy
dc.subject 116 Chemical sciences
dc.subject Rhazya stricta
dc.subject terpenoid indole alkaloids
dc.subject ultra-performance liquid chromatography-mass spectrometry
dc.subject CATHARANTHUS-ROSEUS
dc.subject CELL-CULTURES
dc.subject ALSTONIA-SCHOLARIS
dc.subject SECODINE-TYPE
dc.subject YOHIMBE BARK
dc.subject BIOSYNTHESIS
dc.subject QUANTIFICATION
dc.subject IDENTIFICATION
dc.subject EXTRACT
dc.subject LINE
dc.title Analysis of indole alkaloids from Rhazya stricta hairy roots by ultra-performance liquid chromatography-mass spectrometry en
dc.type Article
dc.contributor.organization Faculty of Pharmacy
dc.contributor.organization Division of Pharmaceutical Biosciences
dc.contributor.organization Pharmaceutical Design and Discovery group
dc.contributor.organization Drug Research Program
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.3390/molecules201219873
dc.relation.issn 1420-3049
dc.rights.accesslevel openAccess
dc.type.version publishedVersion
dc.identifier.url http://www.mdpi.com/1420-3049/20/12/19873

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