On the Role of Histamine Receptors in the Regulation of Circadian Rhythms

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Rozov , S V , Porkka-Heiskanen , T & Panula , P 2015 , ' On the Role of Histamine Receptors in the Regulation of Circadian Rhythms ' , PLoS One , vol. 10 , no. 12 , 0144694 , pp. e0144694 . https://doi.org/10.1371/journal.pone.0144694

Title: On the Role of Histamine Receptors in the Regulation of Circadian Rhythms
Author: Rozov, Stanislav V.; Porkka-Heiskanen, Tarja; Panula, Pertti
Contributor: University of Helsinki, Medicum
University of Helsinki, Medicum
University of Helsinki, Medicum
Date: 2015-12-10
Language: eng
Number of pages: 14
Belongs to series: PLoS One
ISSN: 1932-6203
URI: http://hdl.handle.net/10138/159765
Abstract: Several lines of evidence suggest a regulatory role of histamine in circadian rhythms, but little is known about signaling pathways that would be involved in such a putative role. The aim of this study was to examine whether histamine mediates its effects on the circadian system through Hrh1 or Hrh3 receptors. We assessed both diurnal and free-running locomotor activity rhythms of Hrh1(-/-) and Hrh3(-/-) mice. We also determined the expression of Per1, Per2 and Bmal1 genes in the suprachiasmatic nuclei, several areas of the cerebral cortex and striatum under symmetric 24 h light-dark cycle at zeitgeber times 14 and 6 by using radioactive in situ hybridization. We found no differences between Hrh1(-/-) and wild type mice in the length, amplitude and mesor of diurnal and free-running activity rhythms as well as in expression of Per1, Per2 and Bmal1 genes in any of the examined brain structures. The amplitude of free-running activity rhythm of the Hrh3(-/-) mice was significantly flattened, whereas the expression of the clock genes in Hrh3(-/-) mice was similar to the wild type animals in all of the assessed brain structures. Therefore, the knockout of Hrh1 receptor had no effects on the circadian rhythm of spontaneous locomotion, and a knockout of Hrh3 receptor caused a substantial reduction of free-running activity rhythm amplitude, but none of these knockout models affected the expression patterns of the core clock genes in any of the studied brain structures.
Subject: RAT SUPRACHIASMATIC NUCLEUS
CLOCK GENE-EXPRESSION
LOCOMOTOR-ACTIVITY
BEHAVIORAL CHARACTERIZATION
HISTIDINE-DECARBOXYLASE
PORTACAVAL ANASTOMOSIS
H-3 RECEPTORS
PHASE-SHIFTS
BRAIN
NEURONS
3112 Neurosciences
3111 Biomedicine
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