Incomplete but Infectious Vaccinia Virions Are Produced in the Absence of Oncolysis in Feline SCCF1 Cells

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Parviainen , S , Autio , K , Vähä-Koskela , M , Guse , K , Pesonen , S , Rosol , T J , Zhao , F & Hemminki , A 2015 , ' Incomplete but Infectious Vaccinia Virions Are Produced in the Absence of Oncolysis in Feline SCCF1 Cells ' , PLoS One , vol. 10 , no. 3 , 0120496 . https://doi.org/10.1371/journal.pone.0120496

Titel: Incomplete but Infectious Vaccinia Virions Are Produced in the Absence of Oncolysis in Feline SCCF1 Cells
Författare: Parviainen, Suvi; Autio, Karoliina; Vähä-Koskela, Markus; Guse, Kilian; Pesonen, Sari; Rosol, Thomas J.; Zhao, Fang; Hemminki, Akseli
Upphovmannens organisation: Department of Pathology
Transplantation Laboratory
Medicum
Equine and Small Animal Medicine
Datum: 2015-03-23
Språk: eng
Sidantal: 12
Tillhör serie: PLoS One
ISSN: 1932-6203
DOI: https://doi.org/10.1371/journal.pone.0120496
Permanenta länken (URI): http://hdl.handle.net/10138/160333
Abstrakt: Vaccinia virus is a large, enveloped virus of the poxvirus family. It has broad tropism and typically virus replication culminates in accumulation and lytic release of intracellular mature virus (IMV), the most abundant form of infectious virus, as well as release by budding of extracellular enveloped virus (EEV). Vaccinia viruses have been modified to replicate selectively in cancer cells and clinically tested as oncolytic agents. During preclinical screening of relevant cancer targets for a recombinant Western Reserve strain deleted for both copies of the thymidine kinase and vaccinia growth factor genes, we noticed that confluent monolayers of SCCF1 cat squamous carcinoma cells were not destroyed even after prolonged infection. Interestingly, although SCCF1 cells were not killed, they continuously secreted virus into the cell culture supernatant. To investigate this finding further, we performed detailed studies by electron microscopy. Both intracellular and secreted virions showed morphological abnormalities on ultrastructural inspection, suggesting compromised maturation and morphogenesis of vaccinia virus in SCCF1 cells. Our data suggest that SCCF1 cells produce a morphologically abnormal virus which is nevertheless infective, providing new information on the virus-host cell interactions and intracellular biology of vaccinia virus.
Subject: VIRUS
MEMBRANE
SMALLPOX
PROTEIN
KINASE
ENTRY
318 Medical biotechnology
3111 Biomedicine
Referentgranskad: Ja
Licens: cc_by
Användningsbegränsning: openAccess
Parallelpublicerad version: publishedVersion


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