Incomplete but Infectious Vaccinia Virions Are Produced in the Absence of Oncolysis in Feline SCCF1 Cells

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dc.contributor.author Parviainen, Suvi
dc.contributor.author Autio, Karoliina
dc.contributor.author Vähä-Koskela, Markus
dc.contributor.author Guse, Kilian
dc.contributor.author Pesonen, Sari
dc.contributor.author Rosol, Thomas J.
dc.contributor.author Zhao, Fang
dc.contributor.author Hemminki, Akseli
dc.date.accessioned 2016-03-01T11:44:02Z
dc.date.available 2016-03-01T11:44:02Z
dc.date.issued 2015-03-23
dc.identifier.citation Parviainen , S , Autio , K , Vähä-Koskela , M , Guse , K , Pesonen , S , Rosol , T J , Zhao , F & Hemminki , A 2015 , ' Incomplete but Infectious Vaccinia Virions Are Produced in the Absence of Oncolysis in Feline SCCF1 Cells ' , PLoS One , vol. 10 , no. 3 , 0120496 . https://doi.org/10.1371/journal.pone.0120496
dc.identifier.other PURE: 48778794
dc.identifier.other PURE UUID: 1f16e8f6-cac9-4f7b-9978-c12c21342cd1
dc.identifier.other WOS: 000351987300150
dc.identifier.other Scopus: 84925868289
dc.identifier.uri http://hdl.handle.net/10138/160333
dc.description.abstract Vaccinia virus is a large, enveloped virus of the poxvirus family. It has broad tropism and typically virus replication culminates in accumulation and lytic release of intracellular mature virus (IMV), the most abundant form of infectious virus, as well as release by budding of extracellular enveloped virus (EEV). Vaccinia viruses have been modified to replicate selectively in cancer cells and clinically tested as oncolytic agents. During preclinical screening of relevant cancer targets for a recombinant Western Reserve strain deleted for both copies of the thymidine kinase and vaccinia growth factor genes, we noticed that confluent monolayers of SCCF1 cat squamous carcinoma cells were not destroyed even after prolonged infection. Interestingly, although SCCF1 cells were not killed, they continuously secreted virus into the cell culture supernatant. To investigate this finding further, we performed detailed studies by electron microscopy. Both intracellular and secreted virions showed morphological abnormalities on ultrastructural inspection, suggesting compromised maturation and morphogenesis of vaccinia virus in SCCF1 cells. Our data suggest that SCCF1 cells produce a morphologically abnormal virus which is nevertheless infective, providing new information on the virus-host cell interactions and intracellular biology of vaccinia virus. en
dc.format.extent 12
dc.language.iso eng
dc.relation.ispartof PLoS One
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject VIRUS
dc.subject MEMBRANE
dc.subject SMALLPOX
dc.subject PROTEIN
dc.subject KINASE
dc.subject ENTRY
dc.subject 318 Medical biotechnology
dc.subject 3111 Biomedicine
dc.title Incomplete but Infectious Vaccinia Virions Are Produced in the Absence of Oncolysis in Feline SCCF1 Cells en
dc.type Article
dc.contributor.organization Department of Pathology
dc.contributor.organization Transplantation Laboratory
dc.contributor.organization Medicum
dc.contributor.organization Equine and Small Animal Medicine
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1371/journal.pone.0120496
dc.relation.issn 1932-6203
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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