Constitutive STAT3 Phosphorylation in Circulating CD4(+) T Lymphocytes Associates with Disease Activity and Treatment Response in Recent-Onset Rheumatoid Arthritis

Show full item record



Permalink

http://hdl.handle.net/10138/160533

Citation

Kuuliala , K , Kuuliala , A , Koivuniemi , R , Oksanen , S , Hamalainen , M , Moilanen , E , Kautiainen , H , Leirisalo-Repo , M & Repo , H 2015 , ' Constitutive STAT3 Phosphorylation in Circulating CD4(+) T Lymphocytes Associates with Disease Activity and Treatment Response in Recent-Onset Rheumatoid Arthritis ' PLoS One , vol. 10 , no. 9 , 0137385 . DOI: 10.1371/journal.pone.0137385

Title: Constitutive STAT3 Phosphorylation in Circulating CD4(+) T Lymphocytes Associates with Disease Activity and Treatment Response in Recent-Onset Rheumatoid Arthritis
Author: Kuuliala, Krista; Kuuliala, Antti; Koivuniemi, Riitta; Oksanen, Suvi; Hamalainen, Mari; Moilanen, Eeva; Kautiainen, Hannu; Leirisalo-Repo, Marjatta; Repo, Heikki
Contributor: University of Helsinki, Department of Bacteriology and Immunology
University of Helsinki, Department of Bacteriology and Immunology
University of Helsinki, Dep. of Internal Medicine (Institute of Clinical Medicine) (-2009)
University of Helsinki, Department of General Practice and Primary Health Care
University of Helsinki, Department of Medicine
University of Helsinki, Department of Medicine
Date: 2015-09-09
Language: eng
Number of pages: 15
Belongs to series: PLoS One
ISSN: 1932-6203
URI: http://hdl.handle.net/10138/160533
Abstract: The aim of the present study was to examine constitutive signal transducer and activator of transcription 3 (STAT3) phosphorylation in circulating leukocytes as a candidate biomarker in rheumatoid arthritis (RA). 25 patients with recent-onset, untreated RA provided samples for whole blood flow cytometric determination of intracellular STAT3 phosphorylation, expressed as relative fluorescence units. The occurrence of constitutive STAT3 phosphorylation was evaluated by determining proportion of STAT3-phosphorylated cells among different leukocyte subtypes. Plasma levels of interleukin (IL)-6, IL-17 and IL-21 were measured by immunoassay, radiographs of hands and feet were examined and disease activity score (DAS28) was determined. Biomarkers were restudied and treatment response (according to European League Against Rheumatism) was determined after 12 months of treatment with disease-modifying antirheumatic drugs. At baseline, constitutive phosphorylation of STAT3 occurred in CD4(+) T cells of 14 (56%) patients, CD8(+) T cells of 13 (52%) patients, in CD19+ B cells of 7 (28%) patients, and in CD14(+) monocytes of 12 (48%) patients. STAT3 phosphorylation levels of CD4(+) T cells associated with DAS28, and those of all leukocyte subtypes studied associated with erosive disease. The presence of constitutive STAT3 phosphorylation in CD4(+) T lymphocytes, pSTAT3 fluorescence intensity of CD4(+) and CD8(+) T cells and C-reactive protein (CRP) levels at baseline associated with good treatment response. In conclusion, constitutive STAT3 phosphorylation in circulating CD4(+) T cells is common in recent-onset untreated RA and associates with good treatment response in patients characterized by high disease activity and the presence of systemic inflammation.
Subject: BLOOD FLOW-CYTOMETRY
ANTIRHEUMATIC DRUGS
SIGNALING PROFILES
ACUTE-PANCREATITIS
UP-REGULATION
ACTIVATION
CELLS
INFLAMMATION
TRANSCRIPTION
LEUKOCYTES
3121 Internal medicine
3111 Biomedicine
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
journal.pone.0137385.PDF 847.2Kb PDF View/Open

This item appears in the following Collection(s)

Show full item record