Field Evaluation of Malaria Microscopy, Rapid Malaria Tests and Loop-Mediated Isothermal Amplification in a Rural Hospital in South Western Ethiopia

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Cuadros , J , Perez-Tanoira , R , Prieto-Perez , L , Martin-Martin , I , Berzosa , P , Gonzalez , V , Tisiano , G , Balcha , S , Manuel Ramos , J & Gorgolas , M 2015 , ' Field Evaluation of Malaria Microscopy, Rapid Malaria Tests and Loop-Mediated Isothermal Amplification in a Rural Hospital in South Western Ethiopia ' , PLoS One , vol. 10 , no. 11 , 0142842 . https://doi.org/10.1371/journal.pone.0142842

Title: Field Evaluation of Malaria Microscopy, Rapid Malaria Tests and Loop-Mediated Isothermal Amplification in a Rural Hospital in South Western Ethiopia
Author: Cuadros, Juan; Perez-Tanoira, Ramon; Prieto-Perez, Laura; Martin-Martin, Ines; Berzosa, Pedro; Gonzalez, Vicenta; Tisiano, Gebre; Balcha, Seble; Manuel Ramos, Jose; Gorgolas, Miguel
Contributor: University of Helsinki, Clinicum
Date: 2015-11-10
Language: eng
Number of pages: 8
Belongs to series: PLoS One
ISSN: 1932-6203
URI: http://hdl.handle.net/10138/160560
Abstract: Background In up to one third of the hospitals in some rural areas of Africa, laboratory services in malaria diagnosis are limited to microscopy by thin film, as no capability to perform thick film exists (gold standard in terms of sensitivity for malaria diagnosis). A new rapid molecular malaria diagnostic test called Loop-mediated isothermal DNA amplification (LAMP) has been recently validated in clinical trials showing exceptional sensitivity and specificity features. It could be a reliable diagnostic tool to be implemented without special equipment or training. Objective The objective of this proof of concept study was to confirm the feasibility of using LAMP technique for diagnosis of malaria in a rural Ethiopian hospital with limited resources. Methodology/Principal Findings This study was carried out in Gambo General Hospital, West Arsi Province (Ethiopia), from November 1st to December 31st 2013. A total of 162 patients with a non-focal febrile syndrome were investigated. The diagnostic capability (sensitivity, specificity, positive predictive and negative predictive values) of rapid malaria tests and microscopy by thin film was evaluated in comparison with LAMP. Eleven (6.79%) out of the 162 patients with fever and suspected malaria, tested positive for LAMP, 3 (1.85%) for rapid malaria tests and none of the eleven cases was detected by thin film microscopy. Conclusions/Significance LAMP can be performed in basic rural laboratories without the need for specialized infrastructure and it may set a reliable tool for malaria control to detect a low level parasitemia.
Subject: PLASMODIUM-FALCIPARUM
DIAGNOSIS
PARASITES
POINT
VIVAX
ASSAY
LAMP
KIT
3111 Biomedicine
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