Saccharification of lignocelluloses by carbohydrate active enzymes of the white rot fungus Dichomitus squalens

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Rytioja , J T , Hilden , S K , Mäkinen , S , Vehmaanperä , J , Hatakka , A I & Mäkelä , M R 2015 , ' Saccharification of lignocelluloses by carbohydrate active enzymes of the white rot fungus Dichomitus squalens ' , PLoS One , vol. 10 , no. 12 , e0145166 . https://doi.org/10.1371/journal.pone.0145166

Title: Saccharification of lignocelluloses by carbohydrate active enzymes of the white rot fungus Dichomitus squalens
Author: Rytioja, Johanna Tuulikki; Hilden, Sari Kristiina; Mäkinen, Susanna; Vehmaanperä, Jari; Hatakka, Annele Inkeri; Mäkelä, Miia Riitta
Contributor: University of Helsinki, Department of Food and Nutrition
University of Helsinki, Department of Food and Nutrition
University of Helsinki, Department of Food and Nutrition
University of Helsinki, Department of Food and Nutrition
Date: 2015-12-14
Language: eng
Number of pages: 13
Belongs to series: PLoS One
ISSN: 1932-6203
URI: http://hdl.handle.net/10138/160568
Abstract: White rot fungus Dichomitus squalens is an efficient lignocellulose degrading basidiomycete and a promising source for new plant cell wall polysaccharides depolymerizing enzymes. In this work, we focused on cellobiohydrolases (CBHs) of D. squalens. The native CBHI fraction of the fungus, consisting three isoenzymes, was purified and it maintained the activity for 60 min at 50°C, and was stable in acidic pH. Due to the lack of enzyme activity assay for detecting only CBHII activity, CBHII of D. squalens was produced recombinantly in an industrially important ascomycete host, Trichoderma reesei. CBH enzymes of D. squalens showed potential in hydrolysis of complex lignocellulose substrates sugar beet pulp and wheat bran, and microcrystalline cellulose, Avicel. Recombinant CBHII (rCel6A) of D. squalens hydrolysed all the studied plant biomasses. Compared to individual activities, synergistic effect between rCel6A and native CBHI fraction of D. squalens was significant in the hydrolysis of Avicel. Furthermore, the addition of laccase to the mixture of CBHI fraction and rCel6A significantly enhanced the amount of released reducing sugars from sugar beet pulp. Especially, synergy between individual enzymes is a crucial factor in the tailor-made enzyme mixtures needed for hydrolysis of different plant biomass feedstocks. Our data supports the importance of oxidoreductases in improved enzyme cocktails for lignocellulose saccharification.
Subject: 1183 Plant biology, microbiology, virology
219 Environmental biotechnology
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