Saccharification of lignocelluloses by carbohydrate active enzymes of the white rot fungus Dichomitus squalens

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dc.contributor University of Helsinki, Department of Food and Nutrition en
dc.contributor University of Helsinki, Department of Food and Nutrition en
dc.contributor University of Helsinki, Department of Food and Nutrition en
dc.contributor University of Helsinki, Department of Food and Nutrition en
dc.contributor.author Rytioja, Johanna Tuulikki
dc.contributor.author Hilden, Sari Kristiina
dc.contributor.author Mäkinen, Susanna
dc.contributor.author Vehmaanperä, Jari
dc.contributor.author Hatakka, Annele Inkeri
dc.contributor.author Mäkelä, Miia Riitta
dc.date.accessioned 2016-03-08T12:40:01Z
dc.date.available 2016-03-08T12:40:01Z
dc.date.issued 2015-12-14
dc.identifier.citation Rytioja , J T , Hilden , S K , Mäkinen , S , Vehmaanperä , J , Hatakka , A I & Mäkelä , M R 2015 , ' Saccharification of lignocelluloses by carbohydrate active enzymes of the white rot fungus Dichomitus squalens ' , PLoS One , vol. 10 , no. 12 , e0145166 . https://doi.org/10.1371/journal.pone.0145166 en
dc.identifier.issn 1932-6203
dc.identifier.other PURE: 57116224
dc.identifier.other PURE UUID: 90e7da19-07af-4ee8-83b8-669173fec187
dc.identifier.other Scopus: 84961361142
dc.identifier.other WOS: 000366715900189
dc.identifier.other ORCID: /0000-0001-7056-3931/work/32673731
dc.identifier.other ORCID: /0000-0003-0771-2329/work/28760825
dc.identifier.uri http://hdl.handle.net/10138/160568
dc.description.abstract White rot fungus Dichomitus squalens is an efficient lignocellulose degrading basidiomycete and a promising source for new plant cell wall polysaccharides depolymerizing enzymes. In this work, we focused on cellobiohydrolases (CBHs) of D. squalens. The native CBHI fraction of the fungus, consisting three isoenzymes, was purified and it maintained the activity for 60 min at 50°C, and was stable in acidic pH. Due to the lack of enzyme activity assay for detecting only CBHII activity, CBHII of D. squalens was produced recombinantly in an industrially important ascomycete host, Trichoderma reesei. CBH enzymes of D. squalens showed potential in hydrolysis of complex lignocellulose substrates sugar beet pulp and wheat bran, and microcrystalline cellulose, Avicel. Recombinant CBHII (rCel6A) of D. squalens hydrolysed all the studied plant biomasses. Compared to individual activities, synergistic effect between rCel6A and native CBHI fraction of D. squalens was significant in the hydrolysis of Avicel. Furthermore, the addition of laccase to the mixture of CBHI fraction and rCel6A significantly enhanced the amount of released reducing sugars from sugar beet pulp. Especially, synergy between individual enzymes is a crucial factor in the tailor-made enzyme mixtures needed for hydrolysis of different plant biomass feedstocks. Our data supports the importance of oxidoreductases in improved enzyme cocktails for lignocellulose saccharification. en
dc.format.extent 13
dc.language.iso eng
dc.relation.ispartof PLoS One
dc.rights en
dc.subject 1183 Plant biology, microbiology, virology en
dc.subject 219 Environmental biotechnology en
dc.title Saccharification of lignocelluloses by carbohydrate active enzymes of the white rot fungus Dichomitus squalens en
dc.type Article
dc.description.version Peer reviewed
dc.identifier.doi https://doi.org/10.1371/journal.pone.0145166
dc.type.uri info:eu-repo/semantics/other
dc.type.uri info:eu-repo/semantics/publishedVersion
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