Quantification of Estrogen Receptor-Alpha Expression in Human Breast Carcinomas With a Miniaturized, Low-Cost Digital Microscope: A Comparison with a High-End Whole Slide-Scanner : PLoS One

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Holmström , O , Linder , N , Lundin , M , Moilanen , H , Suutala , A , Turkki , R , Joensuu , H , Isola , J , Diwan , V & Lundin , J 2015 , ' Quantification of Estrogen Receptor-Alpha Expression in Human Breast Carcinomas With a Miniaturized, Low-Cost Digital Microscope: A Comparison with a High-End Whole Slide-Scanner : PLoS One ' , PLoS One , vol. 10 , no. 12 , 0144688 . https://doi.org/10.1371/journal.pone.0144688

Title: Quantification of Estrogen Receptor-Alpha Expression in Human Breast Carcinomas With a Miniaturized, Low-Cost Digital Microscope: A Comparison with a High-End Whole Slide-Scanner : PLoS One
Author: Holmström, Oscar; Linder, Nina; Lundin, Mikael; Moilanen, Hannu; Suutala, Antti; Turkki, Riku; Joensuu, Heikki; Isola, Jorma; Diwan, Vinod; Lundin, Johan
Contributor: University of Helsinki, Institute for Molecular Medicine Finland
University of Helsinki, Institute for Molecular Medicine Finland
University of Helsinki, Institute for Molecular Medicine Finland
University of Helsinki, Institute for Molecular Medicine Finland
University of Helsinki, Institute for Molecular Medicine Finland
University of Helsinki, Translational Cancer Biology (TCB) Research Programme
University of Helsinki, Institute for Molecular Medicine Finland
Date: 2015
Language: eng
Number of pages: 16
Belongs to series: PLoS One
ISSN: 1932-6203
URI: http://hdl.handle.net/10138/160569
Abstract: INTRODUCTION: A significant barrier to medical diagnostics in low-resource environments is the lack of medical care and equipment. Here we present a low-cost, cloud-connected digital microscope for applications at the point-of-care. We evaluate the performance of the device in the digital assessment of estrogen receptor-alpha (ER) expression in breast cancer samples. Studies suggest computer-assisted analysis of tumor samples digitized with whole slide-scanners may be comparable to manual scoring, here we study whether similar results can be obtained with the device presented. MATERIALS AND METHODS: A total of 170 samples of human breast carcinoma, immunostained for ER expression, were digitized with a high-end slide-scanner and the point-of-care microscope. Corresponding regions from the samples were extracted, and ER status was determined visually and digitally. Samples were classified as ER negative (
Subject: 3122 Cancers
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