Free digital image analysis software helps to resolve equivocal scores in HER2 immunohistochemistry

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http://hdl.handle.net/10138/161070

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Helin , H O , Tuominen , V J , Ylinen , O , Helin , H & Isola , J 2016 , ' Free digital image analysis software helps to resolve equivocal scores in HER2 immunohistochemistry ' , Virchows Archiv , vol. 468 , no. 2 , pp. 191-198 . https://doi.org/10.1007/s00428-015-1868-7

Title: Free digital image analysis software helps to resolve equivocal scores in HER2 immunohistochemistry
Author: Helin, Henrik O.; Tuominen, Vilppu J.; Ylinen, Onni; Helin, Heikki; Isola, Jorma
Contributor: University of Helsinki, Department of Pathology
Date: 2016-02
Language: eng
Number of pages: 8
Belongs to series: Virchows Archiv
ISSN: 0945-6317
URI: http://hdl.handle.net/10138/161070
Abstract: Evaluation of human epidermal growth factor receptor 2 (HER2) immunohistochemistry (IHC) is subject to interobserver variation and lack of reproducibility. Digital image analysis (DIA) has been shown to improve the consistency and accuracy of the evaluation and its use is encouraged in current testing guidelines. We studied whether digital image analysis using a free software application (ImmunoMembrane) can assist in interpreting HER2 IHC in equivocal 2+ cases. We also compared digital photomicrographs with whole-slide images (WSI) as material for ImmunoMembrane DIA. We stained 750 surgical resection specimens of invasive breast cancers immunohistochemically for HER2 and analysed staining with ImmunoMembrane. The ImmunoMembrane DIA scores were compared with the originally responsible pathologists' visual scores, a researcher's visual scores and in situ hybridisation (ISH) results. The originally responsible pathologists reported 9.1 % positive 3+ IHC scores, for the researcher this was 8.4 % and for ImmunoMembrane 9.5 %. Equivocal 2+ scores were 34 % for the pathologists, 43.7 % for the researcher and 10.1 % for ImmunoMembrane. Negative 0/1+ scores were 57.6 % for the pathologists, 46.8 % for the researcher and 80.8 % for ImmunoMembrane. There were six false positive cases, which were classified as 3+ by ImmunoMembrane and negative by ISH. Six cases were false negative defined as 0/1+ by IHC and positive by ISH. ImmunoMembrane DIA using digital photomicrographs and WSI showed almost perfect agreement. In conclusion, digital image analysis by ImmunoMembrane can help to resolve a majority of equivocal 2+ cases in HER2 IHC, which reduces the need for ISH testing.
Subject: Computer-assisted image analysis
Digital pathology
Whole-slide imaging
HER2
Immunohistochemistry
Breast cancer
HER2-POSITIVE BREAST-CANCER
IN-SITU HYBRIDIZATION
CLINICAL-ONCOLOGY/COLLEGE
AMERICAN-SOCIETY
RECEPTOR
PATHOLOGY
RECOMMENDATIONS
REPRODUCIBILITY
CONCORDANCE
EXPRESSION
3111 Biomedicine
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