NOD-like receptor signaling and inflammasome-related pathways are highlighted in psoriatic epidermis

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http://hdl.handle.net/10138/161087

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Tervaniemi , M H , Katayama , S , Skoog , T , Siitonen , H A , Vuola , J , Nuutila , K , Sormunen , R , Johnsson , A , Linnarsson , S , Suomela , S , Kankuri , E , Kere , J & Elomaa , O 2016 , ' NOD-like receptor signaling and inflammasome-related pathways are highlighted in psoriatic epidermis ' , Scientific Reports , vol. 6 , 22745 . https://doi.org/10.1038/srep22745

Title: NOD-like receptor signaling and inflammasome-related pathways are highlighted in psoriatic epidermis
Author: Tervaniemi, Mari H.; Katayama, Shintaro; Skoog, Tiina; Siitonen, H. Annika; Vuola, Jyrki; Nuutila, Kristo; Sormunen, Raija; Johnsson, Anna; Linnarsson, Sten; Suomela, Sari; Kankuri, Esko; Kere, Juha; Elomaa, Outi
Contributor: University of Helsinki, Research Programs Unit
University of Helsinki, Research Programs Unit
University of Helsinki, Clinicum
University of Helsinki, Medicum
University of Helsinki, Clinicum
University of Helsinki, Medicum
University of Helsinki, Research Programs Unit
University of Helsinki, Research Programs Unit
Date: 2016-03-15
Language: eng
Number of pages: 12
Belongs to series: Scientific Reports
ISSN: 2045-2322
URI: http://hdl.handle.net/10138/161087
Abstract: Psoriatic skin differs distinctly from normal skin by its thickened epidermis. Most gene expression comparisons utilize full-thickness biopsies, with substantial amount of dermis. We assayed the transcriptomes of normal, lesional, and non-lesional psoriatic epidermis, sampled as split-thickness skin grafts, with 5'-end RNA sequencing. We found that psoriatic epidermis contains more mRNA per total RNA than controls, and took this into account in the bioinformatic analysis. The approach highlighted innate immunity-related pathways in psoriasis, including NOD-like receptor (NLR) signaling and inflammasome activation. We demonstrated that the NLR signaling genes NOD2, PYCARD, CARD6, and IFI16 are upregulated in psoriatic epidermis, and strengthened these findings by protein expression. Interestingly, PYCARD, the key component of the inflammasome, showed an altered expression pattern in the lesional epidermis. The profiling of non-lesional skin highlighted PSORS4 and mitochondrially encoded transcripts, suggesting that their gene expression is altered already before the development of lesions. Our data suggest that all components needed for the active inflammasome are present in the keratinocytes of psoriatic skin. The characterization of inflammasome pathways provides further opportunities for therapy. Complementing previous transcriptome studies, our approach gives deeper insight into the gene regulation in psoriatic epidermis.
Subject: RNA-SEQ
SUSCEPTIBILITY LOCI
PROVIDES INSIGHTS
TRANSCRIPTOME
GENES
EXPRESSION
INNATE
ASSOCIATION
RESPONSES
IMMUNITY
3111 Biomedicine
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