REG4 Is Highly Expressed in Mucinous Ovarian Cancer : A Potential Novel Serum Biomarker

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Lehtinen , L , Vesterkvist , P , Roering , P , Korpela , T , Hattara , L , Kaipio , K , Mpindi , J-P , Hynninen , J , Auranen , A , Davidson , B , Haglund , C , Iljin , K , Grenman , S , Siitari , H & Carpen , O 2016 , ' REG4 Is Highly Expressed in Mucinous Ovarian Cancer : A Potential Novel Serum Biomarker ' , PLoS One , vol. 11 , no. 3 , 0151590 .

Title: REG4 Is Highly Expressed in Mucinous Ovarian Cancer : A Potential Novel Serum Biomarker
Author: Lehtinen, Laura; Vesterkvist, Pia; Roering, Pia; Korpela, Taina; Hattara, Liisa; Kaipio, Katja; Mpindi, John-Patrick; Hynninen, Johanna; Auranen, Annika; Davidson, Ben; Haglund, Caj; Iljin, Kristiina; Grenman, Seija; Siitari, Harri; Carpen, Olli
Contributor organization: Institute for Molecular Medicine Finland
Olli-Pekka Kallioniemi / Principal Investigator
Translational Cancer Biology (TCB) Research Programme
Department of Surgery
II kirurgian klinikka
Research Programs Unit
Precision Cancer Pathology
Date: 2016-03-16
Language: eng
Number of pages: 13
Belongs to series: PLoS One
ISSN: 1932-6203
Abstract: Preoperative diagnostics of ovarian neoplasms rely on ultrasound imaging and the serum biomarkers CA125 and HE4. However, these markers may be elevated in non-neoplastic conditions and may fail to identify most non-serous epithelial cancer subtypes. The objective of this study was to identify histotype-specific serum biomarkers for mucinous ovarian cancer. The candidate genes with mucinous histotype specific expression profile were identified from publicly available gene-expression databases and further in silico data mining was performed utilizing the MediSapiens database. Candidate biomarker validation was done using qRT-PCR, western blotting and immunohistochemical staining of tumor tissue microarrays. The expression level of the candidate gene in serum was compared to the serum CA125 and HE4 levels in a patient cohort of prospectively collected advanced ovarian cancer. Database searches identified REG4 as a potential biomarker with specificity for the mucinous ovarian cancer subtype. The specific expression within epithelial ovarian tumors was further confirmed by mRNA analysis. Immunohistochemical staining of ovarian tumor tissue arrays showed distinctive cytoplasmic expression pattern only in mucinous carcinomas and suggested differential expression between benign and malignant mucinous neoplasms. Finally, an ELISA based serum biomarker assay demonstrated increased expression only in patients with mucinous ovarian cancer. This study identifies REG4 as a potential serum biomarker for histotype-specific detection of mucinous ovarian cancer and suggests serum REG4 measurement as a non-invasive diagnostic tool for postoperative follow-up of patients with mucinous ovarian cancer.
3123 Gynaecology and paediatrics
3122 Cancers
3111 Biomedicine
Peer reviewed: Yes
Usage restriction: openAccess
Self-archived version: publishedVersion

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