The N-Terminal GYPSY Motif Is Required for Pilin-Specific Sortase SrtC1 Functionality in Lactobacillus rhamnosus Strain GG

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Douillard , F P , Rasinkangas , P , Bhattacharjee , A , Palva , A & de Vos , W M 2016 , ' The N-Terminal GYPSY Motif Is Required for Pilin-Specific Sortase SrtC1 Functionality in Lactobacillus rhamnosus Strain GG ' , PLoS One , vol. 11 , no. 4 , 0153373 . https://doi.org/10.1371/journal.pone.0153373

Title: The N-Terminal GYPSY Motif Is Required for Pilin-Specific Sortase SrtC1 Functionality in Lactobacillus rhamnosus Strain GG
Author: Douillard, Francois P.; Rasinkangas, Pia; Bhattacharjee, Arnab; Palva, Airi; de Vos, Willem M.
Contributor: University of Helsinki, Departments of Faculty of Veterinary Medicine
University of Helsinki, Departments of Faculty of Veterinary Medicine
University of Helsinki, Medicum
University of Helsinki, Departments of Faculty of Veterinary Medicine
University of Helsinki, Medicum
Date: 2016-04-12
Language: eng
Number of pages: 18
Belongs to series: PLoS One
ISSN: 1932-6203
URI: http://hdl.handle.net/10138/161446
Abstract: Predominantly identified in pathogenic Gram-positive bacteria, sortase-dependent pili are also found in commensal species, such as the probiotic-marketed strain Lactobacillus rhamnosus strain GG. Pili are typically associated with host colonization, immune signalling and biofilm formation. Comparative analysis of the N-terminal domains of pilin-specific sortases from various piliated Gram-positive bacteria identified a conserved motif, called GYPSY, within the signal sequence. We investigated the function and role of the GYPSY residues by directed mutagenesis in homologous (rod-shaped) and heterologous (coccoid-shaped) expression systems for pilus formation. Substitutions of some of the GYPSY residues, and more specifically the proline residue, were found to have a direct impact on the degree of piliation of Lb. rhamnosus GG. The present findings uncover a new signalling element involved in the functionality of pilin-specific sortases controlling the pilus biogenesis of Lb. rhamnosus GG and related piliated Gram-positive species.
Subject: COMPLETE GENOME SEQUENCE
GRAM-POSITIVE BACTERIA
ENTEROCOCCUS-FAECIUM ISOLATE
LACTIC-ACID BACTERIA
SURFACE-PROTEINS
STREPTOCOCCUS-AGALACTIAE
STAPHYLOCOCCUS-AUREUS
LACTOCOCCUS-LACTIS
CORYNEBACTERIUM-DIPHTHERIAE
SUBSTRATE-SPECIFICITY
3111 Biomedicine
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