Douillard , F P , Rasinkangas , P , Bhattacharjee , A , Palva , A & de Vos , W M 2016 , ' The N-Terminal GYPSY Motif Is Required for Pilin-Specific Sortase SrtC1 Functionality in Lactobacillus rhamnosus Strain GG ' , PLoS One , vol. 11 , no. 4 , 0153373 . https://doi.org/10.1371/journal.pone.0153373
Title: | The N-Terminal GYPSY Motif Is Required for Pilin-Specific Sortase SrtC1 Functionality in Lactobacillus rhamnosus Strain GG |
Author: | Douillard, Francois P.; Rasinkangas, Pia; Bhattacharjee, Arnab; Palva, Airi; de Vos, Willem M. |
Contributor organization: | Departments of Faculty of Veterinary Medicine Veterinary Biosciences Medicum Research Programs Unit Department of Bacteriology and Immunology Immunobiology Research Program Airi Palva / Principal Investigator Veterinary Microbiology and Epidemiology Willem Meindert Vos de / Principal Investigator de Vos & Salonen group |
Date: | 2016-04-12 |
Language: | eng |
Number of pages: | 18 |
Belongs to series: | PLoS One |
ISSN: | 1932-6203 |
DOI: | https://doi.org/10.1371/journal.pone.0153373 |
URI: | http://hdl.handle.net/10138/161446 |
Abstract: | Predominantly identified in pathogenic Gram-positive bacteria, sortase-dependent pili are also found in commensal species, such as the probiotic-marketed strain Lactobacillus rhamnosus strain GG. Pili are typically associated with host colonization, immune signalling and biofilm formation. Comparative analysis of the N-terminal domains of pilin-specific sortases from various piliated Gram-positive bacteria identified a conserved motif, called GYPSY, within the signal sequence. We investigated the function and role of the GYPSY residues by directed mutagenesis in homologous (rod-shaped) and heterologous (coccoid-shaped) expression systems for pilus formation. Substitutions of some of the GYPSY residues, and more specifically the proline residue, were found to have a direct impact on the degree of piliation of Lb. rhamnosus GG. The present findings uncover a new signalling element involved in the functionality of pilin-specific sortases controlling the pilus biogenesis of Lb. rhamnosus GG and related piliated Gram-positive species. |
Subject: |
COMPLETE GENOME SEQUENCE
GRAM-POSITIVE BACTERIA ENTEROCOCCUS-FAECIUM ISOLATE LACTIC-ACID BACTERIA SURFACE-PROTEINS STREPTOCOCCUS-AGALACTIAE STAPHYLOCOCCUS-AUREUS LACTOCOCCUS-LACTIS CORYNEBACTERIUM-DIPHTHERIAE SUBSTRATE-SPECIFICITY 3111 Biomedicine |
Peer reviewed: | Yes |
Rights: | cc_by |
Usage restriction: | openAccess |
Self-archived version: | publishedVersion |
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