The N-Terminal GYPSY Motif Is Required for Pilin-Specific Sortase SrtC1 Functionality in Lactobacillus rhamnosus Strain GG

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Douillard , F P , Rasinkangas , P , Bhattacharjee , A , Palva , A & de Vos , W M 2016 , ' The N-Terminal GYPSY Motif Is Required for Pilin-Specific Sortase SrtC1 Functionality in Lactobacillus rhamnosus Strain GG ' , PLoS One , vol. 11 , no. 4 , 0153373 . https://doi.org/10.1371/journal.pone.0153373

Title: The N-Terminal GYPSY Motif Is Required for Pilin-Specific Sortase SrtC1 Functionality in Lactobacillus rhamnosus Strain GG
Author: Douillard, Francois P.; Rasinkangas, Pia; Bhattacharjee, Arnab; Palva, Airi; de Vos, Willem M.
Contributor organization: Departments of Faculty of Veterinary Medicine
Veterinary Biosciences
Medicum
Research Programs Unit
Department of Bacteriology and Immunology
Immunobiology Research Program
Airi Palva / Principal Investigator
Veterinary Microbiology and Epidemiology
Willem Meindert Vos de / Principal Investigator
de Vos & Salonen group
Date: 2016-04-12
Language: eng
Number of pages: 18
Belongs to series: PLoS One
ISSN: 1932-6203
DOI: https://doi.org/10.1371/journal.pone.0153373
URI: http://hdl.handle.net/10138/161446
Abstract: Predominantly identified in pathogenic Gram-positive bacteria, sortase-dependent pili are also found in commensal species, such as the probiotic-marketed strain Lactobacillus rhamnosus strain GG. Pili are typically associated with host colonization, immune signalling and biofilm formation. Comparative analysis of the N-terminal domains of pilin-specific sortases from various piliated Gram-positive bacteria identified a conserved motif, called GYPSY, within the signal sequence. We investigated the function and role of the GYPSY residues by directed mutagenesis in homologous (rod-shaped) and heterologous (coccoid-shaped) expression systems for pilus formation. Substitutions of some of the GYPSY residues, and more specifically the proline residue, were found to have a direct impact on the degree of piliation of Lb. rhamnosus GG. The present findings uncover a new signalling element involved in the functionality of pilin-specific sortases controlling the pilus biogenesis of Lb. rhamnosus GG and related piliated Gram-positive species.
Subject: COMPLETE GENOME SEQUENCE
GRAM-POSITIVE BACTERIA
ENTEROCOCCUS-FAECIUM ISOLATE
LACTIC-ACID BACTERIA
SURFACE-PROTEINS
STREPTOCOCCUS-AGALACTIAE
STAPHYLOCOCCUS-AUREUS
LACTOCOCCUS-LACTIS
CORYNEBACTERIUM-DIPHTHERIAE
SUBSTRATE-SPECIFICITY
3111 Biomedicine
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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