Faecal Metaproteomic Analysis Reveals a Personalized and Stable Functional Microbiome and Limited Effects of a Probiotic Intervention in Adults

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dc.contributor.author Kolmeder, Carolin A.
dc.contributor.author Salojarvi, Jarkko
dc.contributor.author Ritari, Jarmo
dc.contributor.author de Been, Mark
dc.contributor.author Raes, Jeroen
dc.contributor.author Falony, Gwen
dc.contributor.author Vieira-Silva, Sara
dc.contributor.author Kekkonen, Riina A.
dc.contributor.author Corthals, Garry L.
dc.contributor.author Palva, Airi
dc.contributor.author Salonen, Anne
dc.contributor.author de Vos, Willem M.
dc.date.accessioned 2016-05-11T21:05:00Z
dc.date.available 2016-05-11T21:05:00Z
dc.date.issued 2016-04-12
dc.identifier.citation Kolmeder , C A , Salojarvi , J , Ritari , J , de Been , M , Raes , J , Falony , G , Vieira-Silva , S , Kekkonen , R A , Corthals , G L , Palva , A , Salonen , A & de Vos , W M 2016 , ' Faecal Metaproteomic Analysis Reveals a Personalized and Stable Functional Microbiome and Limited Effects of a Probiotic Intervention in Adults ' , PLoS One , vol. 11 , no. 4 , 0153294 . https://doi.org/10.1371/journal.pone.0153294
dc.identifier.other PURE: 63397494
dc.identifier.other PURE UUID: 0431b05a-e1bd-478f-bd9d-d679fa363260
dc.identifier.other WOS: 000373900700048
dc.identifier.other Scopus: 84963768231
dc.identifier.other ORCID: /0000-0002-6960-7447/work/45240588
dc.identifier.uri http://hdl.handle.net/10138/161860
dc.description.abstract Recent metagenomic studies have demonstrated that the overall functional potential of the intestinal microbiome is rather conserved between healthy individuals. Here we assessed the biological processes undertaken in-vivo by microbes and the host in the intestinal tract by conducting a metaproteome analysis from a total of 48 faecal samples of 16 healthy adults participating in a placebo-controlled probiotic intervention trial. Half of the subjects received placebo and the other half consumed Lactobacillus rhamnosus GG for three weeks (10(10) cfu per day). Faecal samples were collected just before and at the end of the consumption phase as well as after a three-week follow-up period, and were processed for microbial composition and metaproteome analysis. A common core of shared microbial protein functions could be identified in all subjects. Furthermore, we observed marked differences in expressed proteins between subjects that resulted in the definition of a stable and personalized microbiome both at the mass-spectrometry-based proteome level and the functional level based on the KEGG pathway analysis. No significant changes in the metaproteome were attributable to the probiotic intervention. A detailed taxonomic assignment of peptides and comparison to phylogenetic microarray data made it possible to evaluate the activity of the main phyla as well as key species, including Faecalibacterium prausnitzii. Several correlations were identified between human and bacterial proteins. Proteins of the human host accounted for approximately 14% of the identified metaproteome and displayed variations both between and within individuals. The individually different human intestinal proteomes point to personalized host-microbiota interactions. Our findings indicate that analysis of the intestinal metaproteome can complement gene-based analysis and contributes to a thorough understanding of the activities of the microbiome and the relevant pathways in health and disease. en
dc.format.extent 23
dc.language.iso eng
dc.relation.ispartof PLoS One
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject INTESTINAL ALKALINE-PHOSPHATASE
dc.subject LACTOBACILLUS-RHAMNOSUS GG
dc.subject HUMAN GUT MICROBIOME
dc.subject REAL-TIME PCR
dc.subject HEALTHY-ADULTS
dc.subject DISEASE
dc.subject BACTERIAL
dc.subject TRACT
dc.subject HOST
dc.subject IDENTIFICATION
dc.subject 3111 Biomedicine
dc.title Faecal Metaproteomic Analysis Reveals a Personalized and Stable Functional Microbiome and Limited Effects of a Probiotic Intervention in Adults en
dc.type Article
dc.contributor.organization Departments of Faculty of Veterinary Medicine
dc.contributor.organization Veterinary Biosciences
dc.contributor.organization Airi Palva / Principal Investigator
dc.contributor.organization Willem Meindert Vos de / Principal Investigator
dc.contributor.organization Medicum
dc.contributor.organization Department of Bacteriology and Immunology
dc.contributor.organization Immunobiology Research Program
dc.contributor.organization Research Programs Unit
dc.contributor.organization Veterinary Microbiology and Epidemiology
dc.contributor.organization Viikki Plant Science Centre (ViPS)
dc.contributor.organization de Vos & Salonen group
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1371/journal.pone.0153294
dc.relation.issn 1932-6203
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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