Compared to casein, bovine lactoferrin reduces plasma leptin and corticosterone and affects hypothalamic gene expression without altering weight gain or fat mass in high fat diet fed C57/BL6J mice

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McManus , B , Korpela , R , O'Connor , P , Schellekens , H , Cryan , J F , Cotter , P D & Nilaweera , K N 2015 , ' Compared to casein, bovine lactoferrin reduces plasma leptin and corticosterone and affects hypothalamic gene expression without altering weight gain or fat mass in high fat diet fed C57/BL6J mice ' , Nutrition & Metabolism , vol. 12 , 53 . https://doi.org/10.1186/s12986-015-0049-7

Title: Compared to casein, bovine lactoferrin reduces plasma leptin and corticosterone and affects hypothalamic gene expression without altering weight gain or fat mass in high fat diet fed C57/BL6J mice
Author: McManus, Bettina; Korpela, Riitta; O'Connor, Paula; Schellekens, Harriet; Cryan, John F.; Cotter, Paul D.; Nilaweera, Kanishka N.
Contributor: University of Helsinki, Medicum
Date: 2015-12-08
Language: eng
Number of pages: 12
Belongs to series: Nutrition & Metabolism
ISSN: 1743-7075
URI: http://hdl.handle.net/10138/162329
Abstract: Background: Several studies in both humans and rodents have examined the use of lactoferrin as a dietary solution to weight gain and visceral fat accretion and have shown promising results in the short term (up to 7 weeks). This study examined the effects of giving lactoferrin over a longer period of time. Methods: For 13 weeks, male C57/BL6J mice were given a diet containing 10 % kJ fat and 20 % kJ casein (LFD) or a diet with 45 % kJ fat and either 20 % kJ casein (HFD) or 20 % kJ lactoferrin (HFD + Lac). Physiological, metabolic, and biochemical parameters were investigated. Gene expression was investigated by Real-Time PCR and microarray. All data was assessed using t-test, ANOVA or ANCOVA. Gene Set Enrichment Analysis was used to interpret microarray data and assess the impact on gene sets with common biological roles. Results: By the end of the trial, HFD + Lac fed mice did not alter energy balance, body composition, bodyweight, or weight gain when compared to the HFD group. Notably, there were no changes in subcutaneous or epididymal adipose leptin mRNA levels between high fat diet groups, however plasma leptin was significantly reduced in the HFD + Lac compared to HFD group (P <0.05) suggesting reduced leptin secretion. Global microarray analysis of the hypothalamus indicate an overall reduction in gene sets associated with feeding behaviour (P <0.01) and an upregulation of gene sets associated with retinol metabolism in the HFD + Lac group compared to the HFD group (P <0.01). Genes in the latter catergory have been shown to impact on the hypothalamic-pituitary-adrenal axis. Notably, plasma corticosterone levels in the HFD + Lac group were reduced compared to the HFD fed mice (P <0.05). Conclusions: The data suggests that prolonged feeding of full-length dietary lactoferrin, as part of a high fat diet, does not have a beneficial impact on weight gain when compared to casein. However, its impact on leptin secretion and accompanying changes in hypothalamic gene expression may underlie how this dietary protein alters plasma corticosterone. The lactoferrin fed mouse model could be used to identify leptin and corticosterone regulated genes in the hypothalamus without the confounding effects of body weight change.
Subject: Lactoferrin
High fat diet
Weight gain
Fat mass
Obesity
Hypothalamus
Microarray
Leptin
Corticosterone
PITUITARY-ADRENAL AXIS
ENERGY-BALANCE
UP-REGULATION
POMC NEURONS
RATS
RESTRICTION
ADIPOCYTES
METABOLISM
INSULIN
OBESITY
3111 Biomedicine
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