Capturing complex tumour biology in vitro : histological and molecular characterisation of precision cut slices

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Davies , E , Dong , M , Gutekunst , M , Narhi , K , van Zoggel , H J A A , Blom , S , Nagaraj , A , Metsalu , T , Oswald , E , Erkens-Schulze , S , San Martin , J A D , Turkki , R , Wedge , S R , af Hallstrom , T M , Schueler , J , van Weerden , W M , Verschuren , E W , Barry , S T , van der Kuip , H & Hickman , J A 2015 , ' Capturing complex tumour biology in vitro : histological and molecular characterisation of precision cut slices ' , Scientific Reports , vol. 5 , 17187 . https://doi.org/10.1038/srep17187

Title: Capturing complex tumour biology in vitro : histological and molecular characterisation of precision cut slices
Author: Davies, Emma; Dong, Meng; Gutekunst, Matthias; Narhi, Katja; van Zoggel, Hanneke J. A. A.; Blom, Sami; Nagaraj, Ashwini; Metsalu, Tauno; Oswald, Eva; Erkens-Schulze, Sigrun; San Martin, Juan A. Delgado; Turkki, Riku; Wedge, Stephen R.; af Hallstrom, Taija M.; Schueler, Julia; van Weerden, Wytske M.; Verschuren, Emmy W.; Barry, Simon T.; van der Kuip, Heiko; Hickman, John A.
Contributor: University of Helsinki, Institute for Molecular Medicine Finland
University of Helsinki, Institute for Molecular Medicine Finland
University of Helsinki, Institute for Molecular Medicine Finland
University of Helsinki, Institute for Molecular Medicine Finland
University of Helsinki, Institute for Molecular Medicine Finland
University of Helsinki, Institute for Molecular Medicine Finland
Date: 2015-12-09
Language: eng
Number of pages: 17
Belongs to series: Scientific Reports
ISSN: 2045-2322
URI: http://hdl.handle.net/10138/162421
Abstract: Precision-cut slices of in vivo tumours permit interrogation in vitro of heterogeneous cells from solid tumours together with their native microenvironment. They offer a low throughput but high content in vitro experimental platform. Using mouse models as surrogates for three common human solid tumours, we describe a standardised workflow for systematic comparison of tumour slice cultivation methods and a tissue microarray-based method to archive them. Cultivated slices were compared to their in vivo source tissue using immunohistochemical and transcriptional biomarkers, particularly of cellular stress. Mechanical slicing induced minimal stress. Cultivation of tumour slices required organotypic support materials and atmospheric oxygen for maintenance of integrity and was associated with significant temporal and loco-regional changes in protein expression, for example HIF-1 alpha. We recommend adherence to the robust workflow described, with recognition of temporal-spatial changes in protein expression before interrogation of tumour slices by pharmacological or other means.
Subject: HUMAN PROSTATE
ORGANOTYPIC CULTURE
CANCER MODELS
HYPOXIA
VIVO
HETEROGENEITY
EXPRESSION
TISSUES
CELLS
MCF-7
3111 Biomedicine
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