Gene expression profiling of pre-eclamptic placentae by RNA sequencing

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Kaartokallio , T , Cervera , A , Kyllonen , A , Laivuori , K , Kere , J , Laivuori , H & FINNPEC Core Invest Grp 2015 , ' Gene expression profiling of pre-eclamptic placentae by RNA sequencing ' , Scientific Reports , vol. 5 , 14107 .

Title: Gene expression profiling of pre-eclamptic placentae by RNA sequencing
Author: Kaartokallio, Tea; Cervera, Alejandra; Kyllonen, Anjuska; Laivuori, Krista; Kere, Juha; Laivuori, Hannele; FINNPEC Core Invest Grp
Contributor organization: Medicum
Department of Medical and Clinical Genetics
Pregnancy and Genes
Research Programs Unit
Genome-Scale Biology (GSB) Research Program
Sampsa Hautaniemi / Principal Investigator
Pekka Heino / Principal Investigator
Juha Kere / Principal Investigator
Research Programme for Molecular Neurology
Institute for Molecular Medicine Finland
Department of Obstetrics and Gynecology
HUS Gynecology and Obstetrics
Date: 2015-09-21
Language: eng
Number of pages: 15
Belongs to series: Scientific Reports
ISSN: 2045-2322
Abstract: Pre-eclampsia is a common and complex pregnancy disorder that often involves impaired placental development. In order to identify altered gene expression in pre-eclamptic placenta, we sequenced placental transcriptomes of nine pre-eclamptic and nine healthy pregnant women in pools of three. The differential gene expression was tested both by including all the pools in the analysis and by excluding some of the pools based on phenotypic characteristics. From these analyses, we identified altogether 53 differently expressed genes, a subset of which was validated by qPCR in 20 cases and 19 controls. Furthermore, we conducted pathway and functional analyses which revealed disturbed vascular function and immunological balance in pre-eclamptic placenta. Some of the genes identified in our study have been reported by numerous microarray studies (BHLHE40, FSTL3, HK2, HTRA4, LEP, PVRL4, SASH1, SIGLEC6), but many have been implicated in only few studies or have not previously been linked to pre-eclampsia (ARMS2, BTNL9, CCSAP, DIO2, FER1L4, HPSE, LOC100129345, LYN, MYO7B, NCMAP, NDRG1, NRIP1, PLIN2, SBSPON, SERPINB9, SH3BP5, TET3, TPBG, ZNF175). Several of the molecules produced by these genes may have a role in the pathogenesis of pre-eclampsia, and some could qualify as biomarkers for prediction or detection of this pregnancy complication.
Subject: PCR DATA
3111 Biomedicine
3123 Gynaecology and paediatrics
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion

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