Gene expression profiling of pre-eclamptic placentae by RNA sequencing

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Kaartokallio , T , Cervera , A , Kyllonen , A , Laivuori , K , Kere , J , Laivuori , H & FINNPEC Core Invest Grp 2015 , ' Gene expression profiling of pre-eclamptic placentae by RNA sequencing ' , Scientific Reports , vol. 5 , 14107 . https://doi.org/10.1038/srep14107

Title: Gene expression profiling of pre-eclamptic placentae by RNA sequencing
Author: Kaartokallio, Tea; Cervera, Alejandra; Kyllonen, Anjuska; Laivuori, Krista; Kere, Juha; Laivuori, Hannele; FINNPEC Core Invest Grp
Contributor: University of Helsinki, Medicum
University of Helsinki, Research Programs Unit
University of Helsinki, Department of Medical and Clinical Genetics
University of Helsinki, Research Programs Unit
University of Helsinki, Institute for Molecular Medicine Finland
Date: 2015-09-21
Language: eng
Number of pages: 15
Belongs to series: Scientific Reports
ISSN: 2045-2322
URI: http://hdl.handle.net/10138/162431
Abstract: Pre-eclampsia is a common and complex pregnancy disorder that often involves impaired placental development. In order to identify altered gene expression in pre-eclamptic placenta, we sequenced placental transcriptomes of nine pre-eclamptic and nine healthy pregnant women in pools of three. The differential gene expression was tested both by including all the pools in the analysis and by excluding some of the pools based on phenotypic characteristics. From these analyses, we identified altogether 53 differently expressed genes, a subset of which was validated by qPCR in 20 cases and 19 controls. Furthermore, we conducted pathway and functional analyses which revealed disturbed vascular function and immunological balance in pre-eclamptic placenta. Some of the genes identified in our study have been reported by numerous microarray studies (BHLHE40, FSTL3, HK2, HTRA4, LEP, PVRL4, SASH1, SIGLEC6), but many have been implicated in only few studies or have not previously been linked to pre-eclampsia (ARMS2, BTNL9, CCSAP, DIO2, FER1L4, HPSE, LOC100129345, LYN, MYO7B, NCMAP, NDRG1, NRIP1, PLIN2, SBSPON, SERPINB9, SH3BP5, TET3, TPBG, ZNF175). Several of the molecules produced by these genes may have a role in the pathogenesis of pre-eclampsia, and some could qualify as biomarkers for prediction or detection of this pregnancy complication.
Subject: PCR DATA
ONSET PREECLAMPSIA
GROWTH RESTRICTION
PREGNANCIES
SEQ
MICROARRAY
RISK
POPULATION
ARTERIES
CELLS
3111 Biomedicine
3123 Gynaecology and paediatrics
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