Ultrafast excited-state dynamics and fluorescence deactivation of near-infrared fluorescent proteins engineered from bacteriophytochromes

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http://hdl.handle.net/10138/162437

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Zhu , J , Shcherbakova , D M , Hontani , Y , Verkhusha , V V & Kennis , J T M 2015 , ' Ultrafast excited-state dynamics and fluorescence deactivation of near-infrared fluorescent proteins engineered from bacteriophytochromes ' , Scientific Reports , vol. 5 , 12840 . https://doi.org/10.1038/srep12840

Title: Ultrafast excited-state dynamics and fluorescence deactivation of near-infrared fluorescent proteins engineered from bacteriophytochromes
Author: Zhu, Jingyi; Shcherbakova, Daria M.; Hontani, Yusaku; Verkhusha, Vladislav V.; Kennis, John T. M.
Contributor: University of Helsinki, Medicum
Date: 2015-08-06
Language: eng
Number of pages: 8
Belongs to series: Scientific Reports
ISSN: 2045-2322
URI: http://hdl.handle.net/10138/162437
Abstract: Near-infrared fluorescent proteins, iRFPs, are recently developed genetically encoded fluorescent probes for deep-tissue in vivo imaging. Their functions depend on the corresponding fluorescence efficiencies and electronic excited state properties. Here we report the electronic excited state deactivation dynamics of the most red-shifted iRFPs: iRFP702, iRFP713 and iRFP720. Complementary measurements by ultrafast broadband fluorescence and absorption spectroscopy show that single exponential decays of the excited state with 600 similar to 700 ps dominate in all three iRFPs, while photoinduced isomerization was completely inhibited. Significant kinetic isotope effects (KIE) were observed with a factor of similar to 1.8 in D2O, and are interpreted in terms of an excited-state proton transfer (ESPT) process that deactivates the excited state in competition with fluorescence and chromophore mobility. On this basis, new approaches for rational molecular engineering may be applied to iRFPs to improve their fluorescence.
Subject: CYANOBACTERIAL PHYTOCHROME CPH1
CHROMOPHORE-BINDING DOMAIN
RADIATIONLESS TRANSITIONS
BACTERIAL PHYTOCHROME
QUANTUM YIELD
SPECTROSCOPY
PARAMETERS
3111 Biomedicine
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