RNA-Sequencing Reveals the Progression of Phage-Host Interactions between phi R1-37 and Yersinia enterocolitica

Show full item record



Permalink

http://hdl.handle.net/10138/162443

Citation

Leskinen , K , Blasdel , B G , Lavigne , R & Skurnik , M 2016 , ' RNA-Sequencing Reveals the Progression of Phage-Host Interactions between phi R1-37 and Yersinia enterocolitica ' , Viruses-Basel , vol. 8 , no. 4 , 111 . https://doi.org/10.3390/v8040111

Title: RNA-Sequencing Reveals the Progression of Phage-Host Interactions between phi R1-37 and Yersinia enterocolitica
Author: Leskinen, Katarzyna; Blasdel, Bob G.; Lavigne, Rob; Skurnik, Mikael
Contributor: University of Helsinki, Research Programs Unit
University of Helsinki, Medicum
Date: 2016-04
Language: eng
Number of pages: 15
Belongs to series: Viruses-Basel
ISSN: 1999-4915
URI: http://hdl.handle.net/10138/162443
Abstract: Despite the expanding interest in bacterial viruses (bacteriophages), insights into the intracellular development of bacteriophage and its impact on bacterial physiology are still scarce. Here we investigate during lytic infection the whole-genome transcription of the giant phage vB_YecM_phi R1-37 (phi R1-37) and its host, the gastroenteritis causing bacterium Yersinia enterocolitica. RNA sequencing reveals that the gene expression of phi R1-37 does not follow a pattern typical observed in other lytic bacteriophages, as only selected genes could be classified as typically early, middle or late genes. The majority of the genes appear to be expressed constitutively throughout infection. Additionally, our study demonstrates that transcription occurs mainly from the positive strand, while the negative strand encodes only genes with low to medium expression levels. Interestingly, we also detected the presence of antisense RNA species, as well as one non-coding intragenic RNA species. Gene expression in the phage-infected cell is characterized by the broad replacement of host transcripts with phage transcripts. However, the host response in the late phase of infection was also characterized by up-regulation of several specific bacterial gene products known to be involved in stress response and membrane stability, including the Cpx pathway regulators, ATP-binding cassette (ABC) transporters, phage- and cold-shock proteins.
Subject: Yersinia enterocolitica
bacteriophage
phi R1-37
transcriptome
LIPOPOLYSACCHARIDE OUTER CORE
BACTERIOPHAGE T4 DEVELOPMENT
O-SPECIFIC POLYSACCHARIDE
SMALL REGULATORY RNAS
GIANT BACTERIOPHAGE
ESCHERICHIA-COLI
SEROTYPE O3
PHI-KZ
GENOME
THERAPY
3111 Biomedicine
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
viruses_08_00111.pdf 1.809Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record