Mining microbial metatranscriptomes for expression of antibiotic resistance genes under natural conditions

Show simple item record Versluis, Dennis D'Andrea, Marco Maria Garcia, Javier Ramiro Leimena, Milkha M. Hugenholtz, Floor Zhang, Jing Ozturk, Basak Nylund, Lotta Sipkema, Detmer van Schaik, Willem de Vos, Willem M. Kleerebezem, Michiel Smidt, Hauke van Passel, Mark W. J. 2016-05-19T13:30:01Z 2016-05-19T13:30:01Z 2015-07-08
dc.identifier.citation Versluis , D , D'Andrea , M M , Garcia , J R , Leimena , M M , Hugenholtz , F , Zhang , J , Ozturk , B , Nylund , L , Sipkema , D , van Schaik , W , de Vos , W M , Kleerebezem , M , Smidt , H & van Passel , M W J 2015 , ' Mining microbial metatranscriptomes for expression of antibiotic resistance genes under natural conditions ' , Scientific Reports , vol. 5 , 11981 .
dc.identifier.other PURE: 52294955
dc.identifier.other PURE UUID: 91b8d591-c512-43e3-ae5c-60b793b459c2
dc.identifier.other WOS: 000357600300001
dc.identifier.other Scopus: 84936816949
dc.description.abstract Antibiotic resistance genes are found in a broad range of ecological niches associated with complex microbiota. Here we investigated if resistance genes are not only present, but also transcribed under natural conditions. Furthermore, we examined the potential for antibiotic production by assessing the expression of associated secondary metabolite biosynthesis gene clusters. Metatranscriptome datasets from intestinal microbiota of four human adults, one human infant, 15 mice and six pigs, of which only the latter have received antibiotics prior to the study, as well as from sea bacterioplankton, a marine sponge, forest soil and sub-seafloor sediment, were investigated. We found that resistance genes are expressed in all studied ecological niches, albeit with niche-specific differences in relative expression levels and diversity of transcripts. For example, in mice and human infant microbiota predominantly tetracycline resistance genes were expressed while in human adult microbiota the spectrum of expressed genes was more diverse, and also included beta-lactam, aminoglycoside and macrolide resistance genes. Resistance gene expression could result from the presence of natural antibiotics in the environment, although we could not link it to expression of corresponding secondary metabolites biosynthesis clusters. Alternatively, resistance gene expression could be constitutive, or these genes serve alternative roles besides antibiotic resistance. en
dc.format.extent 10
dc.language.iso eng
dc.relation.ispartof Scientific Reports
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject BACTERIAL
dc.subject SOIL
dc.subject DIVERSITY
dc.subject SEQUENCES
dc.subject RESERVOIR
dc.subject PATHWAYS
dc.subject PIGS
dc.subject 3111 Biomedicine
dc.title Mining microbial metatranscriptomes for expression of antibiotic resistance genes under natural conditions en
dc.type Article
dc.contributor.organization Departments of Faculty of Veterinary Medicine
dc.contributor.organization Veterinary Biosciences
dc.contributor.organization Veterinary Microbiology and Epidemiology
dc.contributor.organization Willem Meindert Vos de / Principal Investigator
dc.contributor.organization Medicum
dc.contributor.organization Department of Bacteriology and Immunology
dc.contributor.organization de Vos & Salonen group
dc.description.reviewstatus Peer reviewed
dc.relation.issn 2045-2322
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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