A Missense Change in the ATG4D Gene Links Aberrant Autophagy to a Neurodegenerative Vacuolar Storage Disease

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Kyostila , K , Syrja , P , Jagannathan , V , Chandrasekar , G , Jokinen , T S , Seppala , E H , Becker , D , Drogemuller , M , Dietschi , E , Drogemuller , C , Lang , J , Steffen , F , Rohdin , C , Jaderlund , K H , Lappalainen , A K , Hahn , K , Wohlsein , P , Baumgartner , W , Henke , D , Oevermann , A , Kere , J , Lohi , H & Leeb , T 2015 , ' A Missense Change in the ATG4D Gene Links Aberrant Autophagy to a Neurodegenerative Vacuolar Storage Disease ' , PLoS Genetics , vol. 11 , no. 4 , 1005169 . https://doi.org/10.1371/journal.pgen.1005169

Title: A Missense Change in the ATG4D Gene Links Aberrant Autophagy to a Neurodegenerative Vacuolar Storage Disease
Author: Kyostila, Kaisa; Syrja, Pernilla; Jagannathan, Vidhya; Chandrasekar, Gayathri; Jokinen, Tarja S; Seppala, Eija H.; Becker, Doreen; Drogemuller, Michaela; Dietschi, Elisabeth; Drogemuller, Cord; Lang, Johann; Steffen, Frank; Rohdin, Cecilia; Jaderlund, Karin H.; Lappalainen, Anu K.; Hahn, Kerstin; Wohlsein, Peter; Baumgartner, Wolfgang; Henke, Diana; Oevermann, Anna; Kere, Juha; Lohi, Hannes; Leeb, Tosso
Contributor organization: Departments of Faculty of Veterinary Medicine
Hannes Tapani Lohi / Principal Investigator
Veterinary Biosciences
Veterinary Genetics
Research Programs Unit
Research Programme for Molecular Neurology
Department of Medical and Clinical Genetics
Medicum
Veterinary Pathology and Parasitology
Antti Sukura / Principal Investigator
Equine and Small Animal Medicine
Research Programme of Molecular Medicine
Juha Kere / Principal Investigator
Petbone – ortopedia, fysioterapia, kivunlievitys
Date: 2015-04
Language: eng
Number of pages: 22
Belongs to series: PLoS Genetics
ISSN: 1553-7390
DOI: https://doi.org/10.1371/journal.pgen.1005169
URI: http://hdl.handle.net/10138/162484
Abstract: Inherited neurodegenerative disorders are debilitating diseases that occur across different species. We have performed clinical, pathological and genetic studies to characterize a novel canine neurodegenerative disease present in the Lagotto Romagnolo dog breed. Affected dogs suffer from progressive cerebellar ataxia, sometimes accompanied by episodic nystagmus and behavioral changes. Histological examination revealed unique pathological changes, including profound neuronal cytoplasmic vacuolization in the nervous system, as well as spheroid formation and cytoplasmic aggregation of vacuoles in secretory epithelial tissues and mesenchymal cells. Genetic analyses uncovered a missense change, c.1288G>A; p.A430T, in the autophagy-related ATG4D gene on canine chromosome 20 with a highly significant disease association (p = 3.8 x 10(-136)) in a cohort of more than 2300 Lagotto Romagnolo dogs. ATG4D encodes a poorly characterized cysteine protease belonging to themacroautophagy pathway. Accordingly, our histological analyses indicated altered autophagic flux in affected tissues. The knockdown of the zebrafish homologue atg4da resulted in a widespread developmental disturbance and neurodegeneration in the central nervous system. Our study describes a previously unknown canine neurological disease with particular pathological features and implicates the ATG4D protein as an important autophagy mediator in neuronal homeostasis. The canine phenotype serves as a model to delineate the disease-causing pathological mechanism(s) and ATG4D function, and can also be used to explore treatment options. Furthermore, our results reveal a novel candidate gene for human neurodegeneration and enable the development of a genetic test for veterinary diagnostic and breeding purposes.
Subject: UBIQUITIN-PROTEASOME SYSTEM
CASPASE CLEAVAGE
QUALITY-CONTROL
NERVOUS-SYSTEM
PROTEIN
MICE
GENOME
FAMILY
DEGENERATION
DEGRADATION
3111 Biomedicine
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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