Monitoring physiological changes in haloarchaeal cell during virus release

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Svirskaite , J , Oksanen , H M , Daugelavicius , R & Bamford , D H 2016 , ' Monitoring physiological changes in haloarchaeal cell during virus release ' , Viruses-Basel , vol. 8 , no. 3 . https://doi.org/10.3390/v8030059

Title: Monitoring physiological changes in haloarchaeal cell during virus release
Author: Svirskaite, Julija; Oksanen, Hanna M.; Daugelavicius, Rimantas; Bamford, Dennis H.
Contributor: University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
Date: 2016-03
Language: eng
Number of pages: 15
Belongs to series: Viruses-Basel
ISSN: 1999-4915
URI: http://hdl.handle.net/10138/162762
Abstract: The slow rate of adsorption and non-synchronous release of some archaeal viruses have hindered more thorough analyses of the mechanisms of archaeal virus release. To address this deficit, we utilized four viruses that infect Haloarcula hispanica that represent the four virion morphotypes currently known for halophilic euryarchaeal viruses: (1) icosahedral internal membrane-containing SH1; (2) icosahedral tailed HHTV-1; (3) spindle-shaped His1; and (4) pleomorphic His2. To discern the events occurring as the progeny viruses exit, we monitored culture turbidity, as well as viable cell and progeny virus counts of infected and uninfected cultures. In addition to these traditional metrics, we measured three parameters associated with membrane integrity: the binding of the lipophilic anion phenyldicarbaundecaborane, oxygen consumption, and both intra- and extra-cellular ATP levels.
Subject: potentiometry
icosahedral membrane-containing virus SH1
Haloarcula hispanica
icosahedral tailed virus HHTV-1
pleomorphic virus His2
Pleolipoviridae
virus life cycle
Sphaerolipoviridae
spindle-shaped virus His1
STRANDED-DNA-BACTERIOPHAGE
TURRETED ICOSAHEDRAL VIRUS
PHAGE-PHI-H
ARCHAEAL VIRUS
HALOARCULA-HISPANICA
HALOPHILIC ARCHAEA
HOST INTERACTIONS
HYPERSALINE ENVIRONMENTS
LIPOPHILIC ANIONS
CAPSID PROTEINS
1183 Plant biology, microbiology, virology
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