Local corticosterone production and angiotensin-I converting enzyme shedding in a mouse model of intestinal inflammation

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dc.contributor.author Salmenkari, Hanne
dc.contributor.author Issakainen, Tomi
dc.contributor.author Vapaatalo, Heikki
dc.contributor.author Korpela, Riitta
dc.date.accessioned 2016-06-16T08:18:01Z
dc.date.available 2016-06-16T08:18:01Z
dc.date.issued 2015-09-21
dc.identifier.citation Salmenkari , H , Issakainen , T , Vapaatalo , H & Korpela , R 2015 , ' Local corticosterone production and angiotensin-I converting enzyme shedding in a mouse model of intestinal inflammation ' , World Journal of Gastroenterology , vol. 21 , no. 35 , pp. 10072-10079 . https://doi.org/10.3748/wjg.v21.i35.10072
dc.identifier.other PURE: 54470520
dc.identifier.other PURE UUID: 9d219f3a-1977-4918-b13d-15e7249a02bf
dc.identifier.other WOS: 000361771600003
dc.identifier.other Scopus: 84941912181
dc.identifier.other ORCID: /0000-0003-0337-9186/work/53184144
dc.identifier.uri http://hdl.handle.net/10138/163943
dc.description.abstract AIM: To investigate local corticosterone production and angiotensin- I converting enzyme (ACE) protein expression and their interaction in healthy and inflamed intestine. METHODS: Acute intestinal inflammation was induced to six weeks old male Balb/c mice by administration of either 3% or 5% dextran sodium sulfate (DSS) in drinking water for 7 d (n = 12 in each group). Healthy controls (n = 12) were given tap water. Corticosterone production and ACE protein shedding were measured from ex vivo incubates of the small and large intestine using EIA and ELISA, respectively. Morphological changes of the intestinal wall were assessed in hematoxylin-eosin stained tissue preparations of jejunum and distal colon. Effects of angiotensin II, captopril and metyrapone on corticosterone production was assessed by incubating pieces of small intestine of healthy mice in the presence of 0.1, 1 or 10 mu mol/L angiotensin II, 1, 10 or 100 mu mol/L captopril or 1, 10 or 100 mu mol/L metyrapone solutions and measuring corticosterone released to the incubation buffer after 90 min (n = 5 in each group). RESULTS: Both concentrations of DSS induced inflammation and morphological changes in large intestines but not in small intestines. Changes were observed as distortions of the crypt structure, mucosal erosion, immune cell infiltration to the mucosa and submucosal edema. Ex vivo corticosterone production (2.9 +/- 1.0 ng/mL vs 2.0 +/- 0.8 ng/mL, P = 0.034) and ACE shedding (269.2 +/- 97.1 ng/mL vs 175.7 +/- 52.2 ng/mL, P = 0.016) were increased in small intestines in 3% DSS group compared to the controls. In large intestine, corticosterone production was increased compared to the controls in both 3% DSS (229 +/- 81 pg/mL vs 158 +/- 30 pg/mL, P = 0.017) and 5% DSS groups (366 +/- 163 pg/mL vs 158 +/- 30 pg/mL, P = 0.002). Large intestine ACE shedding was increased in 5% DSS group (41.5 +/- 9.0 ng/mL vs 20.9 +/- 5.2 ng/mL, P = 0.034). Angiotensin II treatment augmented corticosterone production in small intestine at concentration of 10 mu mol/L (0.97 +/- 0.21 ng/mg protein vs 0.40 +/- 0.09 ng/mg protein, P = 0.036). CONCLUSION: Intestinal ACE shedding is increased by DSS-induced intestinal inflammation and parallels local corticosterone production. ACE product angiotensin. stimulates corticosterone formation in healthy intestine. en
dc.format.extent 8
dc.language.iso eng
dc.relation.ispartof World Journal of Gastroenterology
dc.rights cc_by_nc
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Dextran sodium sulfate
dc.subject Inflammation
dc.subject Angiotensin-I converting enzyme
dc.subject Local corticosterone
dc.subject Intestine
dc.subject CELLS
dc.subject RECEPTOR
dc.subject SYSTEM
dc.subject ACE2
dc.subject ENDOTHELIUM
dc.subject EXPRESSION
dc.subject MEMBRANE
dc.subject PROTEIN
dc.subject 3111 Biomedicine
dc.subject 3121 General medicine, internal medicine and other clinical medicine
dc.title Local corticosterone production and angiotensin-I converting enzyme shedding in a mouse model of intestinal inflammation en
dc.type Article
dc.contributor.organization Department of Pharmacology
dc.contributor.organization Medicum
dc.contributor.organization Riitta Anneli Korpela / Principal Investigator
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.3748/wjg.v21.i35.10072
dc.relation.issn 1007-9327
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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