Microsphere-based antibody assays for human parvovirus B19V, CMV and T. gondii

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dc.contributor.author Wang, Yilin
dc.contributor.author Hedman, Lea
dc.contributor.author Perdomo, Maria F.
dc.contributor.author Elfaitouri, Amal
dc.contributor.author Bolin-Wiener, Agnes
dc.contributor.author Kumar, Arun
dc.contributor.author Lappalainen, Maija
dc.contributor.author Soderlund-Venermo, Maria
dc.contributor.author Blomberg, Jonas
dc.contributor.author Hedman, Klaus
dc.date.accessioned 2016-06-17T10:23:01Z
dc.date.available 2016-06-17T10:23:01Z
dc.date.issued 2016-01-08
dc.identifier.citation Wang , Y , Hedman , L , Perdomo , M F , Elfaitouri , A , Bolin-Wiener , A , Kumar , A , Lappalainen , M , Soderlund-Venermo , M , Blomberg , J & Hedman , K 2016 , ' Microsphere-based antibody assays for human parvovirus B19V, CMV and T. gondii ' , BMC Infectious Diseases , vol. 16 , 8 . https://doi.org/10.1186/s12879-015-1194-3
dc.identifier.other PURE: 59255931
dc.identifier.other PURE UUID: 634fa807-0218-421e-a07f-9c9ae1a82b7c
dc.identifier.other WOS: 000367790200001
dc.identifier.other Scopus: 84953388207
dc.identifier.other ORCID: /0000-0003-1779-7960/work/40439495
dc.identifier.other ORCID: /0000-0001-5400-1250/work/30541243
dc.identifier.other ORCID: /0000-0002-9031-4251/work/29657444
dc.identifier.other ORCID: /0000-0002-4582-8317/work/29211241
dc.identifier.uri http://hdl.handle.net/10138/164099
dc.description.abstract Background: Human parvovirus B19 (B19V), cytomegalovirus (CMV) and Toxoplasma gondii (T. gondii) may cause intrauterine infections with potentially severe consequences to the fetus. Current serodiagnosis of these infections is based on detection of antibodies most often by EIA and individually for each pathogen. We developed singleplex and multiplex microsphere-based Suspension Immuno Assays (SIAs) for the simultaneous detection of IgG antibodies against B19V, CMV and T. gondii. Methods: We tested the performances of SIAs as compared to in-house and commercial reference assays using serum samples from well-characterized cohorts. Results: The IgG SIAs for CMV and T. gondii showed good concordance with the corresponding Vidas serodiagnostics. The B19V IgG SIA detected IgG in all samples collected >10 days after onset of symptoms and showed high concordance with EIAs (in-house and Biotrin). The serodiagnostics for these three pathogens performed well in multiplex format. Conclusions: We developed singleplex and multiplex IgG SIAs for the detection of anti-B19V,-CMV and -T. gondii antibodies. The SIAs were highly sensitive and specific, and had a wide dynamic range. These components thus should be suitable for construction of a multiplex test for antibody screening during pregnancy. en
dc.format.extent 6
dc.language.iso eng
dc.relation.ispartof BMC Infectious Diseases
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Microsphere-based suspension immuno assay
dc.subject Singleplex and multiplex
dc.subject B19V
dc.subject CMV
dc.subject T. gondii
dc.subject Pregnancy
dc.subject INFECTIONS
dc.subject DIAGNOSIS
dc.subject EPITOPE
dc.subject 3111 Biomedicine
dc.title Microsphere-based antibody assays for human parvovirus B19V, CMV and T. gondii en
dc.type Article
dc.contributor.organization Klaus Hedman / Principal Investigator
dc.contributor.organization Medicum
dc.contributor.organization Department of Virology
dc.contributor.organization Clinicum
dc.contributor.organization Human Parvoviruses: Epidemiology, Molecular Biology and Clinical Impact
dc.contributor.organization Virus infections and immunity
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1186/s12879-015-1194-3
dc.relation.issn 1471-2334
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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