Capturing tumor complexity in vitro : Comparative analysis of 2D and 3D tumor models for drug discovery

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Stock , K , Estrada , M F , Vidic , S , Gjerde , K , Rudisch , A , Santo , V E , Barbier , M , Blom , S , Arundkar , S C , Selvam , I , Osswald , A , Stein , Y , Gruenewald , S , Brito , C , van Weerden , W , Rotter , V , Boghaert , E , Oren , M , Sommergruber , W , Chong , Y , de Hoogt , R & Graeser , R 2016 , ' Capturing tumor complexity in vitro : Comparative analysis of 2D and 3D tumor models for drug discovery ' , Scientific Reports , vol. 6 , 28951 . https://doi.org/10.1038/srep28951

Title: Capturing tumor complexity in vitro : Comparative analysis of 2D and 3D tumor models for drug discovery
Author: Stock, Kristin; Estrada, Marta F.; Vidic, Suzana; Gjerde, Kjersti; Rudisch, Albin; Santo, Vitor E.; Barbier, Michael; Blom, Sami; Arundkar, Sharath C.; Selvam, Irwin; Osswald, Annika; Stein, Yan; Gruenewald, Sylvia; Brito, Catarina; van Weerden, Wytske; Rotter, Varda; Boghaert, Erwin; Oren, Moshe; Sommergruber, Wolfgang; Chong, Yolanda; de Hoogt, Ronald; Graeser, Ralph
Other contributor: University of Helsinki, Institute for Molecular Medicine Finland

Date: 2016-07-01
Language: eng
Number of pages: 15
Belongs to series: Scientific Reports
ISSN: 2045-2322
DOI: https://doi.org/10.1038/srep28951
URI: http://hdl.handle.net/10138/164974
Abstract: Two-dimensional (2D) cell cultures growing on plastic do not recapitulate the three dimensional (3D) architecture and complexity of human tumors. More representative models are required for drug discovery and validation. Here, 2D culture and 3D mono-and stromal co-culture models of increasing complexity have been established and cross-comparisons made using three standard cell carcinoma lines: MCF7, LNCaP, NCI-H1437. Fluorescence-based growth curves, 3D image analysis, immunohistochemistry and treatment responses showed that end points differed according to cell type, stromal co-culture and culture format. The adaptable methodologies described here should guide the choice of appropriate simple and complex in vitro models.
Subject: OVARIAN-CANCER CELLS
BREAST-CANCER
EXTRACELLULAR-MATRIX
SOLID TUMORS
RESISTANCE
GROWTH
FIBROBLASTS
SPHEROIDS
MICROENVIRONMENT
IDENTIFICATION
3111 Biomedicine
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