Capturing tumor complexity in vitro : Comparative analysis of 2D and 3D tumor models for drug discovery

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dc.contributor.author Stock, Kristin
dc.contributor.author Estrada, Marta F.
dc.contributor.author Vidic, Suzana
dc.contributor.author Gjerde, Kjersti
dc.contributor.author Rudisch, Albin
dc.contributor.author Santo, Vitor E.
dc.contributor.author Barbier, Michael
dc.contributor.author Blom, Sami
dc.contributor.author Arundkar, Sharath C.
dc.contributor.author Selvam, Irwin
dc.contributor.author Osswald, Annika
dc.contributor.author Stein, Yan
dc.contributor.author Gruenewald, Sylvia
dc.contributor.author Brito, Catarina
dc.contributor.author van Weerden, Wytske
dc.contributor.author Rotter, Varda
dc.contributor.author Boghaert, Erwin
dc.contributor.author Oren, Moshe
dc.contributor.author Sommergruber, Wolfgang
dc.contributor.author Chong, Yolanda
dc.contributor.author de Hoogt, Ronald
dc.contributor.author Graeser, Ralph
dc.date.accessioned 2016-07-27T06:03:02Z
dc.date.available 2016-07-27T06:03:02Z
dc.date.issued 2016-07-01
dc.identifier.citation Stock , K , Estrada , M F , Vidic , S , Gjerde , K , Rudisch , A , Santo , V E , Barbier , M , Blom , S , Arundkar , S C , Selvam , I , Osswald , A , Stein , Y , Gruenewald , S , Brito , C , van Weerden , W , Rotter , V , Boghaert , E , Oren , M , Sommergruber , W , Chong , Y , de Hoogt , R & Graeser , R 2016 , ' Capturing tumor complexity in vitro : Comparative analysis of 2D and 3D tumor models for drug discovery ' , Scientific Reports , vol. 6 , 28951 . https://doi.org/10.1038/srep28951
dc.identifier.other PURE: 66514291
dc.identifier.other PURE UUID: ed202e48-21d3-491e-885d-caa822eddb29
dc.identifier.other WOS: 000378832900001
dc.identifier.other Scopus: 84976897214
dc.identifier.other ORCID: /0000-0002-1878-8911/work/28930642
dc.identifier.uri http://hdl.handle.net/10138/164974
dc.description.abstract Two-dimensional (2D) cell cultures growing on plastic do not recapitulate the three dimensional (3D) architecture and complexity of human tumors. More representative models are required for drug discovery and validation. Here, 2D culture and 3D mono-and stromal co-culture models of increasing complexity have been established and cross-comparisons made using three standard cell carcinoma lines: MCF7, LNCaP, NCI-H1437. Fluorescence-based growth curves, 3D image analysis, immunohistochemistry and treatment responses showed that end points differed according to cell type, stromal co-culture and culture format. The adaptable methodologies described here should guide the choice of appropriate simple and complex in vitro models. en
dc.format.extent 15
dc.language.iso eng
dc.relation.ispartof Scientific Reports
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject OVARIAN-CANCER CELLS
dc.subject BREAST-CANCER
dc.subject EXTRACELLULAR-MATRIX
dc.subject SOLID TUMORS
dc.subject RESISTANCE
dc.subject GROWTH
dc.subject FIBROBLASTS
dc.subject SPHEROIDS
dc.subject MICROENVIRONMENT
dc.subject IDENTIFICATION
dc.subject 3111 Biomedicine
dc.title Capturing tumor complexity in vitro : Comparative analysis of 2D and 3D tumor models for drug discovery en
dc.type Article
dc.contributor.organization Institute for Molecular Medicine Finland
dc.contributor.organization Olli-Pekka Kallioniemi / Principal Investigator
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1038/srep28951
dc.relation.issn 2045-2322
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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