Discovery of intramolecular trans-sialidases in human gut microbiota suggests novel mechanisms of mucosal adaptation

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http://hdl.handle.net/10138/165261

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Tailford , L E , Owen , C D , Walshaw , J , Crost , E H , Hardy-Goddard , J , Le Gall , G , de Vos , W M , Taylor , G L & Juge , N 2015 , ' Discovery of intramolecular trans-sialidases in human gut microbiota suggests novel mechanisms of mucosal adaptation ' , Nature Communications , vol. 6 , 7624 . https://doi.org/10.1038/ncomms8624

Julkaisun nimi: Discovery of intramolecular trans-sialidases in human gut microbiota suggests novel mechanisms of mucosal adaptation
Tekijä: Tailford, Louise E.; Owen, C. David; Walshaw, John; Crost, Emmanuelle H.; Hardy-Goddard, Jemma; Le Gall, Gwenaelle; de Vos, Willem M.; Taylor, Garry L.; Juge, Nathalie
Tekijän organisaatio: Departments of Faculty of Veterinary Medicine
Willem Meindert Vos de / Principal Investigator
Veterinary Biosciences
Department of Bacteriology and Immunology
Medicum
de Vos & Salonen group
Päiväys: 2015-07
Kieli: eng
Sivumäärä: 12
Kuuluu julkaisusarjaan: Nature Communications
ISSN: 2041-1723
DOI-tunniste: https://doi.org/10.1038/ncomms8624
URI: http://hdl.handle.net/10138/165261
Tiivistelmä: The gastrointestinal mucus layer is colonized by a dense community of microbes catabolizing dietary and host carbohydrates during their expansion in the gut. Alterations in mucosal carbohydrate availability impact on the composition of microbial species. Ruminococcus gnavus is a commensal anaerobe present in the gastrointestinal tract of > 90% of humans and overrepresented in inflammatory bowel diseases (IBD). Using a combination of genomics, enzymology and crystallography, we show that the mucin-degrader R. gnavus ATCC 29149 strain produces an intramolecular trans-sialidase (IT-sialidase) that cleaves off terminal alpha 2-3-linked sialic acid from glycoproteins, releasing 2,7-anhydro-Neu5Ac instead of sialic acid. Evidence of IT-sialidases in human metagenomes indicates that this enzyme occurs in healthy subjects but is more prevalent in IBD metagenomes. Our results uncover a previously unrecognized enzymatic activity in the gut microbiota, which may contribute to the adaptation of intestinal bacteria to the mucosal environment in health and disease.
Avainsanat: INFLAMMATORY BOWEL DISEASES
SIALIC-ACID
STREPTOCOCCUS-PNEUMONIAE
ENTERIC PATHOGENS
CRYSTAL-STRUCTURE
MUCUS LAYERS
MUCIN
BACTERIA
SPECIFICITY
METABOLISM
3111 Biomedicine
Vertaisarvioitu: Kyllä
Tekijänoikeustiedot: cc_by
Pääsyrajoitteet: openAccess
Rinnakkaistallennettu versio: publishedVersion


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