A resilient formin-derived cortical actin meshwork in the rear drives actomyosin-based motility in 2D confinement

Show full item record



Permalink

http://hdl.handle.net/10138/165268

Citation

Ramalingam , N , Franke , C , Jaschinski , E , Winterhoff , M , Lu , Y , Bruehmann , S , Junemann , A , Meier , H , Noegel , A A , Weber , I , Zhao , H , Merkel , R , Schleicher , M & Faix , J 2015 , ' A resilient formin-derived cortical actin meshwork in the rear drives actomyosin-based motility in 2D confinement ' , Nature Communications , vol. 6 , 8496 . https://doi.org/10.1038/ncomms9496

Title: A resilient formin-derived cortical actin meshwork in the rear drives actomyosin-based motility in 2D confinement
Author: Ramalingam, Nagendran; Franke, Christof; Jaschinski, Evelin; Winterhoff, Moritz; Lu, Yao; Bruehmann, Stefan; Junemann, Alexander; Meier, Helena; Noegel, Angelika A.; Weber, Igor; Zhao, Hongxia; Merkel, Rudolf; Schleicher, Michael; Faix, Jan
Contributor: University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
Date: 2015-09
Language: eng
Number of pages: 15
Belongs to series: Nature Communications
ISSN: 2041-1723
URI: http://hdl.handle.net/10138/165268
Abstract: Cell migration is driven by the establishment of disparity between the cortical properties of the softer front and the more rigid rear allowing front extension and actomyosin-based rear contraction. However, how the cortical actin meshwork in the rear is generated remains elusive. Here we identify the mDia1-like formin A (ForA) from Dictyostelium discoideum that generates a subset of filaments as the basis of a resilient cortical actin sheath in the rear. Mechanical resistance of this actin compartment is accomplished by actin crosslinkers and IQGAP-related proteins, and is mandatory to withstand the increased contractile forces in response to mechanical stress by impeding unproductive blebbing in the rear, allowing efficient cell migration in two-dimensional-confined environments. Consistently, ForA supresses the formation of lateral protrusions, rapidly relocalizes to new prospective ends in repolarizing cells and is required for cortical integrity. Finally, we show that ForA utilizes the phosphoinositide gradients in polarized cells for subcellular targeting.
Subject: CELL-MIGRATION
DICTYOSTELIUM-DISCOIDEUM
RAC1 GTPASES
FILOPODIA FORMATION
CLEAVAGE FURROW
MYOSIN-II
CHEMOTAXIS
CYTOSKELETON
PROTEIN
IQGAP1
1182 Biochemistry, cell and molecular biology
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
ncomms9496.pdf 5.850Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record