DNA barcodes identify Central-Asian Colias butterflies (Lepidoptera, Pieridae)

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Laiho , J & Ståhls , G 2013 , ' DNA barcodes identify Central-Asian Colias butterflies (Lepidoptera, Pieridae) ' , ZooKeys , no. 365 , pp. 175-196 . https://doi.org/10.3897/zookeys.365.5879

Title: DNA barcodes identify Central-Asian Colias butterflies (Lepidoptera, Pieridae)
Author: Laiho, Juha; Ståhls, Gunilla
Contributor organization: Zoology
Finnish Museum of Natural History
Date: 2013-12-30
Language: eng
Number of pages: 22
Belongs to series: ZooKeys
ISSN: 1313-2989
DOI: https://doi.org/10.3897/zookeys.365.5879
URI: http://hdl.handle.net/10138/165329
Abstract: A majority of the known Colias species (Lepidoptera: Pieridae, Coliadinae) occur in the mountainous regions of Central-Asia, vast areas that are hard to access, rendering the knowledge of many species limited due to the lack of extensive sampling. Two gene regions, the mitochondrial COI ‘barcode’ region and the nuclear ribosomal protein RpS2 gene region were used for exploring the utility of these DNA markers for species identification. A comprehensive sampling of COI barcodes for Central-Asian Colias butterflies showed that the barcodes facilitated identification of most of the included species. Phylogenetic reconstruction based on parsimony and Neighbor-Joining recovered most species as monophyletic entities. For the RpS2 gene region species-specific sequences were registered for some of the included Colias spp. Nevertheless, this gene region was not deemed useful as additional molecular ‘barcode’. A parsimony analysis of the combined COI and RpS2 data did not support the current subgeneric classification based on morphological characteristics
Subject: Barcoding
1181 Ecology, evolutionary biology
1184 Genetics, developmental biology, physiology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion

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