Bidirectional Interplay between Vimentin Intermediate Filaments and Contractile Actin Stress Fibers

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Jiu , Y , Lehtimaki , J , Tojkander , S , Cheng , F , Jäälinoja , H , Liu , X , Varjosalo , M , Eriksson , J E & Lappalainen , P 2015 , ' Bidirectional Interplay between Vimentin Intermediate Filaments and Contractile Actin Stress Fibers ' , Cell Reports , vol. 11 , no. 10 , pp. 1511-1518 . https://doi.org/10.1016/j.celrep.2015.05.008

Title: Bidirectional Interplay between Vimentin Intermediate Filaments and Contractile Actin Stress Fibers
Author: Jiu, Yaming; Lehtimaki, Jaakko; Tojkander, Sari; Cheng, Fang; Jäälinoja, Harri; Liu, Xiaonan; Varjosalo, Markku; Eriksson, John E.; Lappalainen, Pekka
Other contributor: University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
University of Helsinki, Päivi Ojala / Principal Investigator
University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology



Date: 2015
Language: eng
Number of pages: 8
Belongs to series: Cell Reports
ISSN: 2211-1247
DOI: https://doi.org/10.1016/j.celrep.2015.05.008
URI: http://hdl.handle.net/10138/165444
Abstract: The actin cytoskeleton and cytoplasmic intermediate filaments contribute to cell migration and morphogenesis, but the interplay between these two central cytoskeletal elements has remained elusive. Here, we find that specific actin stress fiber structures, transverse arcs, interact with vimentin intermediate filaments and promote their retrograde flow. Consequently, myosin-II-containing arcs are important for perinuclear localization of the vimentin network in cells. The vimentin network reciprocally restricts retrograde movement of arcs and hence controls the width of flat lamellum at the leading edge of the cell. Depletion of plectin recapitulates the vimentin organization phenotype of arc-deficient cells without affecting the integrity of vimentin filaments or stress fibers, demonstrating that this cytoskeletal crosslinker is required for productive interactions between vimentin and arcs. Collectively, our results reveal that plectin-mediated interplay between contractile actomyosin arcs and vimentin intermediate filaments controls the localization and dynamics of these two cytoskeletal systems and is consequently important for cell morphogenesis.
Subject: CELL-SHAPE
ADHESION
MIGRATION
PLECTIN
MICROTUBULES
RECRUITMENT
MECHANISMS
DYNAMICS
NETWORK
1182 Biochemistry, cell and molecular biology
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