Microbial co-occurrence patterns in deep Precambrian bedrock fracture fluids

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dc.contributor.author Purkamo, Lotta
dc.contributor.author Bomberg, Malin
dc.contributor.author Kietäväinen, Riikka
dc.contributor.author Salavirta, Heikki
dc.contributor.author Nyyssonen, Mari
dc.contributor.author Nuppunen-Puputti, Maija
dc.contributor.author Ahonen, Lasse
dc.contributor.author Kukkonen, Ilmo
dc.contributor.author Itavaara, Merja
dc.date.accessioned 2016-08-10T06:52:02Z
dc.date.available 2016-08-10T06:52:02Z
dc.date.issued 2016
dc.identifier.citation Purkamo , L , Bomberg , M , Kietäväinen , R , Salavirta , H , Nyyssonen , M , Nuppunen-Puputti , M , Ahonen , L , Kukkonen , I & Itavaara , M 2016 , ' Microbial co-occurrence patterns in deep Precambrian bedrock fracture fluids ' , Biogeosciences , vol. 13 , no. 10 , pp. 3091-3108 . https://doi.org/10.5194/bg-13-3091-2016
dc.identifier.other PURE: 67104706
dc.identifier.other PURE UUID: e4f4442b-fda5-4839-9392-f37c207ad01a
dc.identifier.other WOS: 000378354900015
dc.identifier.other Scopus: 84971539862
dc.identifier.other ORCID: /0000-0002-4867-777X/work/127008795
dc.identifier.uri http://hdl.handle.net/10138/165462
dc.description.abstract The bacterial and archaeal community composition and the possible carbon assimilation processes and energy sources of microbial communities in oligotrophic, deep, crystalline bedrock fractures is yet to be resolved. In this study, intrinsic microbial communities from groundwater of six fracture zones from 180 to 2300aEuro-m depths in Outokumpu bedrock were characterized using high-throughput amplicon sequencing and metagenomic prediction. Comamonadaceae-, Anaerobrancaceae- and Pseudomonadaceae-related operational taxonomic units (OTUs) form the core community in deep crystalline bedrock fractures in Outokumpu. Archaeal communities were mainly composed of Methanobacteriaceae-affiliating OTUs. The predicted bacterial metagenomes showed that pathways involved in fatty acid and amino sugar metabolism were common. In addition, relative abundance of genes coding the enzymes of autotrophic carbon fixation pathways in predicted metagenomes was low. This indicates that heterotrophic carbon assimilation is more important for microbial communities of the fracture zones. Network analysis based on co-occurrence of OTUs revealed possible "keystone" genera of the microbial communities belonging to Burkholderiales and Clostridiales. Bacterial communities in fractures resemble those found in oligotrophic, hydrogen-enriched environments. Serpentinization reactions of ophiolitic rocks in Outokumpu assemblage may provide a source of energy and organic carbon compounds for the microbial communities in the fractures. Sulfate reducers and methanogens form a minority of the total microbial communities, but OTUs forming these minor groups are similar to those found in other deep Precambrian terrestrial bedrock environments. en
dc.format.extent 18
dc.language.iso eng
dc.relation.ispartof Biogeosciences
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject 16S RIBOSOMAL-RNA
dc.subject SOUTH-AFRICA
dc.subject NATIONAL-PARK
dc.subject SUBSURFACE
dc.subject COMMUNITIES
dc.subject 1171 Geosciences
dc.title Microbial co-occurrence patterns in deep Precambrian bedrock fracture fluids en
dc.type Article
dc.contributor.organization Department of Physics
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.5194/bg-13-3091-2016
dc.relation.issn 1726-4170
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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