Differences in muscle and adipose tissue gene expression and cardio-metabolic risk factors in the member of physical activity discordant twin pairs

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Leskinen , T , Rinnankoski-Tuikka , R , Rintala , M , Seppanen-Laakso , T , Pollanen , E , Alen , M , Sipila , S , Kaprio , J , Kovanen , V , Rahkila , P , Oresic , M , Kainulainen , H & Kujala , U M 2010 , ' Differences in muscle and adipose tissue gene expression and cardio-metabolic risk factors in the member of physical activity discordant twin pairs ' , PLoS One , vol. 5 , no. 9 , pp. e12609 . https://doi.org/10.1371/journal.pone.0012609

Title: Differences in muscle and adipose tissue gene expression and cardio-metabolic risk factors in the member of physical activity discordant twin pairs
Author: Leskinen, Tuija; Rinnankoski-Tuikka, Rita; Rintala, Mirva; Seppanen-Laakso, Tuulikki; Pollanen, Eija; Alen, Markku; Sipila, Sarianna; Kaprio, Jaakko; Kovanen, Vuokko; Rahkila, Paavo; Oresic, Matej; Kainulainen, Heikki; Kujala, Urho M.
Contributor: University of Helsinki, Department of Public Health
Date: 2010
Language: eng
Number of pages: 9
Belongs to series: PLoS One
ISSN: 1932-6203
URI: http://hdl.handle.net/10138/165611
Abstract: High physical activity/aerobic fitness predicts low morbidity and mortality. Our aim was to identify the most up-regulated gene sets related to long-term physical activity vs. inactivity in skeletal muscle and adipose tissues and to obtain further information about their link with cardio-metabolic risk factors. We studied ten same-sex twin pairs (age range 50-74 years) who had been discordant for leisure-time physical activity for 30 years. The examinations included biopsies from m. vastus lateralis and abdominal subcutaneous adipose tissue. RNA was analyzed with the genome-wide Illumina Human WG-6 v3.0 Expression BeadChip. For pathway analysis we used Gene Set Enrichment Analysis utilizing active vs. inactive co-twin gene expression ratios. Our findings showed that among the physically active members of twin pairs, as compared to their inactive co-twins, gene expression in the muscle tissue samples was chronically up-regulated for the central pathways related to energy metabolism, including oxidative phosphorylation, lipid metabolism and supportive metabolic pathways. Up-regulation of these pathways was associated in particular with aerobic fitness and high HDL cholesterol levels. In fat tissue we found physical activity-associated increases in the expression of polyunsaturated fatty acid metabolism and branched-chain amino acid degradation gene sets both of which associated with decreased 'high-risk' ectopic body fat and plasma glucose levels. Consistent with other findings, plasma lipidomics analysis showed up-regulation of the triacylglycerols containing the polyunsaturated fatty acids. Our findings identified skeletal muscle and fat tissue pathways which are associated with the long-term physical activity and reduced cardio-metabolic disease risk, including increased aerobic fitness. In particular, improved skeletal muscle oxidative energy and lipid metabolism as well as changes in adipocyte function and redistribution of body fat are associated with reduced cardio-metabolic risk.
Subject: INSULIN-RESISTANCE
SKELETAL-MUSCLE
CHRONIC DISEASE
EXERCISE
MORTALITY
TRIACYLGLYCEROL
ADAPTATIONS
SELECTION
CAPACITY
FITNESS
311 Basic medicine
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