Gene Expression Profiling of U12-Type Spliceosome Mutant Drosophila Reveals Widespread Changes in Metabolic Pathways

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Pessa , H K J , Greco , D , Kvist , J , Wahlström , G M , Heino , T I , Auvinen , P & Frilander , M J 2010 , ' Gene Expression Profiling of U12-Type Spliceosome Mutant Drosophila Reveals Widespread Changes in Metabolic Pathways ' , PLoS One , vol. 5 , no. 10 , pp. e13215 . https://doi.org/10.1371/journal.pone.0013215

Title: Gene Expression Profiling of U12-Type Spliceosome Mutant Drosophila Reveals Widespread Changes in Metabolic Pathways
Alternative title: Gene Expression Profiling of U12-Type Spliceosome Mutant Drosophila Reveals Widespread Changes in Metabolic Pathways : PLoS ONE
Author: Pessa, Heli K. J.; Greco, Dario; Kvist, Jouni; Wahlström, Gudrun Margareta; Heino, Tapio I.; Auvinen, Petri; Frilander, Mikko J.
Contributor organization: Institute of Biotechnology
Biosciences
Centre of Excellence in Metapopulation Research
DNA Sequencing and Genomics
Minor spliceosome
Date: 2010
Language: eng
Number of pages: 13
Belongs to series: PLoS One
ISSN: 1932-6203
DOI: https://doi.org/10.1371/journal.pone.0013215
URI: http://hdl.handle.net/10138/165613
Abstract: Background The U12-type spliceosome is responsible for the removal of a subset of introns from eukaryotic mRNAs. U12-type introns are spliced less efficiently than normal U2-type introns, which suggests a rate-limiting role in gene expression. The Drosophila genome contains about 20 U12-type introns, many of them in essential genes, and the U12-type spliceosome has previously been shown to be essential in the fly Methodology/Principal Findings We have used a Drosophila line with a P-element insertion in U6atac snRNA, an essential component of the U12-type spliceosome, to investigate the impact of U12-type introns on gene expression at the organismal level during fly development. This line exhibits progressive accumulation of unspliced U12-type introns during larval development and the death of larvae at the third instar stage. Surprisingly, microarray and RT-PCR analyses revealed that most genes containing U12-type introns showed only mild perturbations in the splicing of U12-type introns. In contrast, we detected widespread downstream effects on genes that do not contain U12-type introns, with genes related to various metabolic pathways constituting the largest group. Conclusions/Significance U12-type intron-containing genes exhibited variable gene-specific responses to the splicing defect, with some genes showing up- or downregulation, while most did not change significantly. The observed residual U12-type splicing activity could be explained with the mutant U6atac allele having a low level of catalytic activity. Detailed analysis of all genes suggested that a defect in the splicing of the U12-type intron of the mitochondrial prohibitin gene may be the primary cause of the various downstream effects detected in the microarray analysis.
Subject: MESSENGER-RNA INTRONS
AT-AC INTRON
U12-DEPENDENT INTRONS
MINOR SPLICEOSOME
IN-VIVO
MITOCHONDRIAL PROTEINS
CELL-PROLIFERATION
PROHIBITIN FAMILY
LIFE-SPAN
U11
118 Biological sciences
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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