Evidence for conserved function of γ–glutamyltranspeptidase in Helicobacter genus

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dc.contributor.author Rossi, Mirko
dc.contributor.author Bolz, Christian
dc.contributor.author Revez, Joana
dc.contributor.author Javed, Sundus
dc.contributor.author El-Najjar, Nahed
dc.contributor.author Anderl, Florian
dc.contributor.author Hyytiäinen, Heidi
dc.contributor.author Vuorela, Pia
dc.contributor.author Gerhard, Markus
dc.contributor.author Hänninen, Marja-Liisa
dc.date.accessioned 2016-08-15T10:43:01Z
dc.date.available 2016-08-15T10:43:01Z
dc.date.issued 2012
dc.identifier.citation Rossi , M , Bolz , C , Revez , J , Javed , S , El-Najjar , N , Anderl , F , Hyytiäinen , H , Vuorela , P , Gerhard , M & Hänninen , M-L 2012 , ' Evidence for conserved function of γ–glutamyltranspeptidase in Helicobacter genus ' , PLoS One , vol. 7 , no. 2 , pp. e30543 . https://doi.org/10.1371/journal.pone.0030543
dc.identifier.other PURE: 21406580
dc.identifier.other PURE UUID: eefc255f-1c56-4398-af76-b033b865f0fc
dc.identifier.other WOS: 000302737400002
dc.identifier.other Scopus: 84856853119
dc.identifier.other ORCID: /0000-0002-1585-6384/work/29950975
dc.identifier.uri http://hdl.handle.net/10138/165749
dc.description.abstract The confounding consequences of Helicobacter bilis infection in experimental mice populations are well recognized, but the role of this bacterium in human diseases is less known. Limited data are available on virulence determinants of this species. In Helicobacter pylori, γ-glutamyltranspeptidase (γGT) contributes to the colonization of the gastric mucosa and to the pathogenesis of peptic ulcer. The role of γGT in H. bilis infections remains unknown. The annotated genome sequence of H. bilis revealed two putative ggt genes and our aim was to characterize these H. bilis γGT paralogues. We performed a phylogenetic analysis to understand the evolution of Helicobacter γGTs and to predict functional activities of these two genes. In addition, both copies of H. bilis γGTs were expressed as recombinant proteins and their biochemical characteristics were analysed. Functional complementation of Escherichia coli deficient in γGT activity and deletion of γGT in H. bilis were performed. Finally, the inhibitory effect of T-cell and gastric cell proliferation by H. bilis γGT was assessed. Our results indicated that one gene is responsible for γGT activity, while the other showed no γGT activity due to lack of autoprocessing. Although both H. bilis and H. pylori γGTs exhibited a similar affinity to L-Glutamine and γ-Glutamyl-p-nitroanilide, the H. bilis γGT was significantly less active. Nevertheless, H. bilis γGT inhibited T-cell proliferation at a similar level to that observed for H. pylori. Finally, we showed a similar suppressive influence of both H. bilis and H. pylori γGTs on AGS cell proliferation mediated by an apoptosis-independent mechanism. Our data suggest a conserved function of γGT in the Helicobacter genus. Since γGT is present only in a few enterohepatic Helicobacter species, its expression appears not to be essential for colonization of the lower gastrointestinal tract, but it could provide metabolic advantages in colonization capability of different niches. en
dc.format.extent 13
dc.language.iso eng
dc.relation.ispartof PLoS One
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject 413 Veterinary science
dc.title Evidence for conserved function of γ–glutamyltranspeptidase in Helicobacter genus en
dc.type Article
dc.contributor.organization Departments of Faculty of Veterinary Medicine
dc.contributor.organization Food Hygiene and Environmental Health
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1371/journal.pone.0030543
dc.relation.issn 1932-6203
dc.rights.accesslevel openAccess
dc.type.version publishedVersion
dc.relation.funder Unknown funder

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