DIC image reconstruction using an energy minimization framework to visualize optical path length distribution

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http://hdl.handle.net/10138/165965

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Koos , K , Molnar , J , Kelemen , L , Tamas , G & Horvath , P 2016 , ' DIC image reconstruction using an energy minimization framework to visualize optical path length distribution ' , Scientific Reports , vol. 6 , 30420 . https://doi.org/10.1038/srep30420

Title: DIC image reconstruction using an energy minimization framework to visualize optical path length distribution
Author: Koos, Krisztian; Molnar, Jozsef; Kelemen, Lorand; Tamas, Gabor; Horvath, Peter
Contributor: University of Helsinki, Institute for Molecular Medicine Finland
Date: 2016-07-25
Language: eng
Number of pages: 9
Belongs to series: Scientific Reports
ISSN: 2045-2322
URI: http://hdl.handle.net/10138/165965
Abstract: Label-free microscopy techniques have numerous advantages such as low phototoxicity, simple setup and no need for fluorophores or other contrast materials. Despite their advantages, most label-free techniques cannot visualize specific cellular compartments or the location of proteins and the image formation limits quantitative evaluation. Differential interference contrast (DIC) is a qualitative microscopy technique that shows the optical path length differences within a specimen. We propose a variational framework for DIC image reconstruction. The proposed method largely outperforms state-of-the-art methods on synthetic, artificial and real tests and turns DIC microscopy into an automated high-content imaging tool. Image sets and the source code of the examined algorithms are made publicly available.
Subject: INTERFERENCE CONTRAST MICROSCOPE
HOLOGRAMS
3111 Biomedicine
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