Viral RNase3 Co-Localizes and Interacts with the Antiviral Defense Protein SGS3 in Plant Cells

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Weinheimer , I , Haikonen , T , Ala-Poikela , M , Moser , M , Streng , J , Rajamaki , M-L & Valkonen , J P T 2016 , ' Viral RNase3 Co-Localizes and Interacts with the Antiviral Defense Protein SGS3 in Plant Cells ' , PLoS One , vol. 11 , no. 7 , 0159080 . https://doi.org/10.1371/journal.pone.0159080

Title: Viral RNase3 Co-Localizes and Interacts with the Antiviral Defense Protein SGS3 in Plant Cells
Author: Weinheimer, Isabel; Haikonen, Tuuli; Ala-Poikela, Marjo; Moser, Mirko; Streng, Janne; Rajamaki, Minna-Liisa; Valkonen, Jari P. T.
Contributor: University of Helsinki, Department of Agricultural Sciences
University of Helsinki, Department of Agricultural Sciences
University of Helsinki, Department of Agricultural Sciences
University of Helsinki, Department of Agricultural Sciences
University of Helsinki, Department of Agricultural Sciences
University of Helsinki, Department of Agricultural Sciences
Date: 2016-07-08
Language: eng
Number of pages: 16
Belongs to series: PLoS One
ISSN: 1932-6203
URI: http://hdl.handle.net/10138/165969
Abstract: Sweet potato chlorotic stunt virus (SPCSV; family Closteroviridae) encodes a Class 1 RNase III endoribonuclease (RNase3) that suppresses post-transcriptional RNA interference (RNAi) and eliminates antiviral defense in sweetpotato plants (Ipomoea batatas). For RNAi suppression, RNase3 cleaves double-stranded small interfering RNAs (ds-siRNA) and long dsRNA to fragments that are too short to be utilized in RNAi. However, RNase3 can suppress only RNAi induced by sense RNA. Sense-mediated RNAi involves host suppressor of gene silencing 3 (SGS3) and RNA-dependent RNA polymerase 6 (RDR6). In this study, subcellular localization and host interactions of RNase3 were studied in plant cells. RNase3 was found to interact with SGS3 of sweetpotato and Arabidopsis thaliana when expressed in leaves, and it localized to SGS3/RDR6 bodies in the cytoplasm of leaf cells and protoplasts. RNase3 was also detected in the nucleus. Co-expression of RNase3 and SGS3 in leaf tissue enhanced the suppression of RNAi, as compared with expression of RNase3 alone. These results suggest additional mechanisms needed for efficient RNase3-mediated suppression of RNAi and provide new information about the subcellular context and phase of the RNAi pathway in which RNase3 realizes RNAi suppression.
Subject: HELPER COMPONENT PROTEINASE
POTATO-VIRUS-A
SILENCING SUPPRESSOR
SIRNA BIOGENESIS
MOSAIC-VIRUS
ARABIDOPSIS
INFECTION
GENE
TRANSITIVITY
RECOGNITION
1183 Plant biology, microbiology, virology
4111 Agronomy
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